scholarly journals SERIAL RECONSTRUCTION OF THE CHARACTERISTIC GRANULE OF THE LANGERHANS CELL

1968 ◽  
Vol 36 (3) ◽  
pp. 595-602 ◽  
Author(s):  
Richard W. Sagebiel ◽  
Thomas H. Reed
Keyword(s):  
Electron Microscope ◽  
Electron Micrographs ◽  
Three Dimensional ◽  
Langerhans Cell ◽  
Human Epidermis ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Three Dimensional Structure ◽  
Three Dimensional Models ◽  
The Many

Three-dimensional models of individual granules in the same Langerhans cell were made after analyzing serial sections of human epidermis in the electron microscope. These models revealed that the granule is made up of a flattened or curved orthogonal net of particles which is bounded externally by a limiting membrane and which may be disc-shaped, cup-shaped, or combinations of both shapes. This variety of shapes accounts for the many configurations of the granule seen in individual electron micrographs. Usually, the granule has a vesicular portion at, or near one margin. This demonstration of the three-dimensional structure of the granule establishes the inaccuracy of previously used descriptive terms, the granule should be called simply the "Langerhans cell granule."

Image Analysis of Electron Micrographs by Computor

1970 ◽  
Vol 28 ◽  
pp. 36-37
Author(s):  
M. Watanabe ◽  
S. Sasaki ◽  
N. Anasawa
Keyword(s):  
Electron Micrographs ◽  
Three Dimensional ◽  
Incident Beam ◽  
Dimensional Structure ◽  
Electron Microscopic ◽  
Three Dimensional Structure ◽  
Intrinsic Nature ◽  
Two Dimensional Image ◽  
Electron Microscopic Technique ◽  
Electron Microscopic Image

Since the electron microscope allows only a two-dimensional image of the specimen to be projected in the direction of the incident beam, it is difficult to represent a three-dimensional arrangement of the specimen substructure from an electron microscopic image. It is also difficult and troublesome to determine the inner specimen structure by means of electron diffraction due to its intrinsic nature. The same is ture of stereo electron microscopic technique. A new method by which one can derive detailed information on the three-dimensional structure of materials is of vital necessity.A method of quantitative determination of three-dimensional structure from electron microscopic images has been reported by De Rosier and Klug. This method makes it possible to derive from electron micrographs, the structures of micro specimens and bio-polymers inadequate for X-ray analysis.

Computer assembly of serial sections

1989 ◽  
Vol 4 ◽  
pp. 157-164 ◽  
Author(s):  
Ralph E. Chapman
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Taxonomic Identification ◽  
Serial Sections ◽  
Three Dimensional Structure ◽  
Internal Structures ◽  
Number Of Authors

The importance of serial sections for the analysis of the morphology of fossil organisms has been stressed by a number of authors (e.g., Westbroek, 1967, 1969; Cooper, 1983; Sandy, 1986, this volume, Chapter 14) because they provide a way to visualize the three-dimensional structure, both internal and external, of specimens that cannot be studied in other ways. The importance of internal structures for the taxonomy of many groups (e.g., the brachidia of brachiopods; Westbroek et al., 1976; Cooper, 1983) makes the study of serial sections imperative in many cases for proper taxonomic identification.

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