scholarly journals THE RIBONUCLEOPROTEIN NATURE OF LARGE PARTICLES IN THE MEIOSPORANGIA OF ALLOMYCES

1957 ◽  
Vol 3 (6) ◽  
pp. 879-888 ◽  
Author(s):  
Edward S. Rorem ◽  
Leonard Machlis
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Oleic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Large Particles ◽  
Significant Change

Particles averaging 3 to 4 µ in diameter, which are called chromospheres and fill the immature meiosporangia of the watermold Allomyces, were isolated and analyzed. The preparations were obtained by repeated centrifugations or by passage of the homogenate into a column of sand saturated with oleic acid, followed by selective elution of the chromospheres with alternate layers of oleic acid and an aqueous solution. The chromospheres contain approximately 12 per cent RNA, no DNA, and 60 per cent protein. It was concluded that they are pure or nearly pure ribonucleoprotein. Analysis of meiosporangia with chromospheres and after the chromospheres have disappeared showed no significant change in RNA or free amino acids. It was concluded that chromosphere disappearance is a fragmentation into small granules. The relation of chromospheres to postmeiotic chromospheres and nuclear caps is discussed. Speculation as to the function of these bodies is presented.

CONSTITUENTS OF THE HEMOLYMPH OF THE LOBSTER, HOMARUS AMERIGANUS MILNE EDWARDS

1966 ◽  
Vol 44 (11) ◽  
pp. 1447-1459 ◽  
Author(s):  
James E. Stewart ◽  
J. R. Dingle ◽  
P. H. Odense
Keyword(s):  
Amino Acids ◽  
Lactic Acid ◽  
Oleic Acid ◽  
Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Homarus Americanus ◽  
Carbohydrate Level ◽  
Stable Characteristic

Electrophoretic and sedimentation analyses were made on the plasma and serum of the hemolymph of the lobster Homarus americanus, and on purified hemocyanin and fibrinogen prepared from these. Amino acid analyses of these two proteins and the plasma, and of the free amino acids of the plasma were also determined. Fibrinogen maintained its function and character upon isolation. It was completely and selectively removed from the plasma by stirring with small amounts of oleic acid. The pH of the hemolymph was 7.6; this was a stable characteristic. The lactic acid in the hemolymph of lobsters held unfed in a disturbing environment at first increased fivefold and then returned to normal; the carbohydrate level remained unchanged.

Betaines and free amino acids in salt stressed vitroplants and winter resting buds of Populus trichocarpa x deltoides

1991 ◽  
Vol 83 (1) ◽  
pp. 136-143 ◽  
Author(s):  
L. Bray ◽  
D. Chriqui ◽  
K. Gloux ◽  
D. Le Rudulier ◽  
M. Meyer ◽  
...  
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Populus Trichocarpa

Plasma and skeletal muscle free amino acids in type I, insulin-treated diabetic subjects

Diabetes ◽  
1985 ◽  
Vol 34 (8) ◽  
pp. 812-815 ◽  
Author(s):  
L. Borghi ◽  
R. Lugari ◽  
A. Montanari ◽  
P. Dall'Argine ◽  
G. F. Elia ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Type I
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