scholarly journals THE NUCLEOLAR CHANNEL SYSTEM OF HUMAN ENDOMETRIUM

1965 ◽  
Vol 27 (2) ◽  
pp. 293-304 ◽  
Author(s):  
John A. Terzakis
Keyword(s):  
Electron Microscope ◽  
Menstrual Cycle ◽  
Amorphous Matrix ◽  
Human Endometrium ◽  
Endometrial Cell ◽  
Unusual Structure ◽  
Channel System ◽  
Dense Granules ◽  
System A

Human endometrium taken during the early to mid-secretory portion of the menstrual cycle is studied with the electron microscope. A description of the nucleolus is given. In addition, an unusual structure within the endometrial cell nucleolus is described, consisting of amorphous matrix, 150-A dense granules, and a series of tubular channels. This structure is named the nucleolar channel system. A description is given of the geometric variability of the nucleolar channel system, its contents, and its relationship to the cytoplasm. The morphologic basis for a nucleolar-cytoplasmic interrelationship via the nucleolar channel system is described. Some of the implications of this relationship are discussed. The work of previous investigators on the nucleolar channel system is discussed.

Bcl-2 and Fas expression in eutopic and ectopic human endometrium during the menstrual cycle in relation to endometrial cell apoptosis

1997 ◽  
Vol 176 (2) ◽  
pp. 360-368 ◽  
Author(s):  
Hirohiko Watanabe ◽  
Hideharu Kanzaki ◽  
Shinji Narukawa ◽  
Takuya Inoue ◽  
Hiroshi Katsuragawa ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Cell Apoptosis ◽  
Human Endometrium ◽  
Endometrial Cell

Fine Structure of Interdental Cells in the Mouse Cochlea

1970 ◽  
Vol 28 ◽  
pp. 140-141
Author(s):  
Roberta M. Bruck
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Young Adult ◽  
Uranyl Acetate ◽  
Ground Substance ◽  
Tectorial Membrane ◽  
Lead Citrate ◽  
Unusual Structure ◽  
Thin Sections ◽  
Collagenous Fibers

An unusual structure in the cochlea is the spiral limbus; this periosteal tissue consists of stellate fibroblasts and collagenous fibers embedded in a translucent ground substance. The collagenous fibers are arranged in vertical columns (the auditory teeth of Haschke). Between the auditory teeth are interdental furrows in which the interdental cells are situated. These epithelial cells supposedly secrete the tectorial membrane.The fine structure of interdental cells in the rat was reported by Iurato (1962). Since the mouse appears to be different, a description of the fine structure of mouse interdental cells' is presented. Young adult C57BL/6J mice were perfused intervascularly with 1% paraformaldehyde/ 1.25% glutaraldehyde in .1M phosphate buffer (pH7.2-7.4). Intact cochlea were decalcified in .1M EDTA by the method of Baird (1967), postosmicated, dehydrated, and embedded in Araldite. Thin sections stained with uranyl acetate and lead citrate were examined in a Phillips EM-200 electron microscope.

Single-cell transcriptomic atlas of the human endometrium during the menstrual cycle

2020 ◽  
Vol 26 (10) ◽  
pp. 1644-1653 ◽  
Author(s):  
Wanxin Wang ◽  
Felipe Vilella ◽  
Pilar Alama ◽  
Inmaculada Moreno ◽  
Marco Mignardi ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Single Cell ◽  
Human Endometrium

scholarly journals An Electron Microscope Study of Basophile Substances of Frozen-Dried Rat Liver

1958 ◽  
Vol 4 (3) ◽  
pp. 291-300 ◽  
Author(s):  
Henry Finck
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Rat Liver ◽  
Microscope Study ◽  
Electron Microscope Study ◽  
Freeze Drying ◽  
Light And Electron Microscopy ◽  
Enzyme Treatment ◽  
Dense Granules ◽  
Homogeneous Matrix

Small pieces of liver from rats subjected to different dietary regimes were fixed by freeze-drying, and postfixed by in vacuo heating and denaturation with alcohol. Specimens were digested with ribo- or deoxyribonuclease, and stained with gallocyanin-chromalum, azure II, the Feulgen procedure or alcoholic platinic tetrabromide. Some specimens were reserved as controls of the effects of enzyme treatment. Stained and unstained specimens were embedded in methacrylate and examined by light and electron microscopy. Basophilic and Feulgen-positive substances, after contact with watery reagents, were found by electron microscopy to exist as small dense granules embedded in a less dense homogeneous matrix, forming the walls of submicroscopic vacuoles. These granules were absent after digestion with nucleodepolymerases. In specimens (unstained, or stained with platinic tetrabromide) which had not passed through water, the dense (basophile) substances in nuclei and cytoplasm were found to exist, not as granules, but as ill defined submicroscopic concentrates which blended imperceptibly into the homogeneous matrix of the vacuolar walls. Objections to the use of stains for improving contrast conditions in electron microscopy of tissues are discussed, and it is concluded that the reagents do not necessarily produce the observed increases in contrast by selectively stabilizing certain structures. The concept of microsomes as pre-existing distinct morphological entities in intact (unhomogenized) cells is thought to be inconsistent with the distribution of basophile substances in frozen-dried liver.

scholarly journals THE SUBMICROSCOPIC ORGANIZATION OF AXON MATERIAL ISOLATED FROM MYELIN NERVE FIBERS

1953 ◽  
Vol 98 (3) ◽  
pp. 269-276 ◽  
Author(s):  
E. De Robertis ◽  
C. M. Franchi
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Phase Contrast ◽  
Nerve Fibers ◽  
Myelinated Nerve ◽  
Myelinated Nerve Fibers ◽  
Dense Granules ◽  
Small Clusters ◽  
Membranous Material ◽  
The Way

A technique has been developed for the extrusion of axon material from myelinated nerve fibers. This material is then compressed and prepared for observation with the electron microscope. All the stages of preparation and purification of the axon material can be checked microscopically and in the present paper they are illustrated with phase contrast photomicrographs. Observation with the electron microscope of the compressed axons showed the presence of the following components: granules, fibrils, and a membranous material. Only the larger granules could be seen with the ordinary microscope. A considerable number of dense granules were observed. Of these the largest resemble typical mitochondria of 250 mµ by 900 mµ. In addition rows or small clusters of dense granules ranging in diameter from 250 to 90 mµ were present. In several specimens fragments of a membrane 120 to 140 A thick and intimately connected with the axon were found. The entire axon appeared to be constituted of a large bundle of parallel tightly packed fibrils among which the granules are interspersed. The fibrils are of indefinite length and generally smooth. They are rather labile structures, less resistant in the rat than in the toad nerve. They varied between 100 and 400 A in diameter and in some cases disintegrated into very fine filaments (less than 100 A thick). The significance is discussed of the submicroscopic structures revealed by electron microscopy of the material prepared in the way described.

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