scholarly journals STRANDEDNESS OF VICIA FABA CHROMOSOMES AS REVEALED BY ENZYME DIGESTION STUDIES

1965 ◽  
Vol 26 (1) ◽  
pp. 125-135 ◽  
Author(s):  
James E. Trosko ◽  
Sheldon Wolff
Keyword(s):  
Vicia Faba ◽  
Light Microscope ◽  
Lateral Roots ◽  
Enzyme Digestion ◽  
Trypsin Digestion ◽  
Mitotic Chromosomes ◽  
Ordinary Light ◽  
Linear Continuity ◽  
Neutral Formalin ◽  
Formalin Fixed

Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contained four strands that could be seen with an ordinary light microscope. The experiments are interpreted as indicating that mitotic chromosomes of Vicia faba are multistranded and that the linear continuity of the chromosome is dependent on protein.

scholarly journals 5-AMINOURACIL TREATMENT

1971 ◽  
Vol 48 (2) ◽  
pp. 248-252 ◽  
Author(s):  
S. H. Socher ◽  
D. Davidson
Keyword(s):  
Mitotic Index ◽  
Vicia Faba ◽  
Cell Population ◽  
Lateral Roots ◽  
Proliferating Cells ◽  
Dividing Cells ◽  
Proliferating Cell ◽  
Two Populations

Treatment of Vicia faba lateral roots with a range of concentrations of 5-aminouracil (5-AU) indicate that cells are stopped at a particular point in interphase. The timing of the fall in mitotic index suggests that cells are held at the S - G2 transition. When cells are held at this point, treatments with 5-AU can be used to estimate the duration of G2 + mitosis/2 of proliferating cells. Treatment with 5-AU can also be used to demonstrate the presence of subpopulations of dividing cells that differ in their G2 duration. Using this method, 5-AU-induced inhibition, we have confirmed that in V. faba lateral roots there are two populations of dividing cells: (a) a fast-dividing population, which makes up ∼85% of the proliferating cell population and has a G2 + mitosis/2 duration of 3.3 hr, and (b) a slow-dividing population, which makes up ∼15% of dividing cells and has a G2 duration in excess of 12 hr. These estimates are similar to those obtained from percentage labeled mitosis (PLM) curves after incorporation of thymidine-3H.

scholarly journals Cytotoxic Effects of Terminalia Chebula on Meiotic & Mitotic Chromosomes of Vicia Faba

2011 ◽  
Vol 4 (7) ◽  
pp. 427-431
Author(s):  
Monika Asthana ◽  
◽  
Rakesh Kumar Sarbhoy ◽  
Surabhi Mahajan ◽  
Avnish Kumar
Keyword(s):  
Vicia Faba ◽  
Terminalia Chebula ◽  
Mitotic Chromosomes ◽  
Cytotoxic Effects
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