scholarly journals Glial SIK3: A central player in ion and volume homeostasis in Drosophila peripheral nerves

2019 ◽  
Vol 218 (12) ◽  
pp. 3888-3889
Author(s):  
Uri Kahanovitch ◽  
Michelle L. Olsen
Keyword(s):  
Signal Transduction ◽  
Electrical Properties ◽  
Peripheral Nerves ◽  
Neuronal Cells ◽  
Extracellular Fluid ◽  
Cell Biol

The electrical properties of neuronal cells rely on gradients of ions across their membranes and the extracellular fluid (ECF) in which they are bathed. Little is known regarding how the ECF volume and content is maintained. In this issue, Li et al. (2019. J. Cell Biol. https://doi.org/10.1083/jcb.201907138) identify the kinase SIK3 in glia as a key signal transduction regulator in ion and volume homeostasis in Drosophila peripheral nerves.

scholarly journals Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins.

1990 ◽  
Vol 111 (6) ◽  
pp. 2785-2794 ◽  
Author(s):  
E Brown ◽  
L Hooper ◽  
T Ho ◽  
H Gresham
Keyword(s):  
Signal Transduction ◽  
Plasma Membrane ◽  
Synthetic Peptides ◽  
Plasma Membranes ◽  
Protein A ◽  
Hematopoietic Cells ◽  
Cell Types ◽  
Cell Biol ◽  
Leukocyte Response ◽  
Stimulation Of

Phagocytosis by monocytes or neutrophils can be enhanced by interaction with several proteins or synthetic peptides containing the Arg-Gly-Asp sequence. Recently we showed that an mAb, B6H12, specifically inhibited this enhancement of neutrophil phagocytosis by inhibiting Arg-Gly-Asp binding to the leukocyte response integrin (Gresham, H. D., J. L. Goodwin, P. M. Allen, D. C. Anderson, and E. J. Brown. 1989. J. Cell Biol. 108:1935-1943). Now, we have purified the antigen recognized by B6H12 to homogeneity. Surprisingly, it is a 50-kD molecule that is expressed on the plasma membranes of all hematopoietic cells, including erythrocytes, which express no known integrins. On platelets and placenta, but not on erythrocytes, this protein is associated with an integrin that can be recognized by an anti-beta 3 antibody. In addition, both the anti-beta 3 and several mAbs recognizing the 50-kD protein inhibit Arg-Gly-Asp stimulation of phagocytosis. These data demonstrate an association between integrins and the 50-kD protein on several cell types. For this reason, we call it Integrin-associated Protein (IAP). We hypothesize that IAP may play a role in signal transduction for enhanced phagocytosis by Arg-Gly-Asp ligands.

scholarly journals Two distinct, calcium-mediated, signal transduction pathways can trigger deflagellation in Chlamydomonas reinhardtii

1994 ◽  
Vol 124 (5) ◽  
pp. 807-815 ◽  
Author(s):  
LM Quarmby ◽  
HC Hartzell
Keyword(s):  
Signal Transduction ◽  
Chlamydomonas Reinhardtii ◽  
Living Cells ◽  
Signalling Pathways ◽  
Signal Transduction Pathways ◽  
Channel Blockers ◽  
Wasp Venom ◽  
Intracellular Pool ◽  
Cell Biol ◽  
Molecular Machinery

The molecular machinery of deflagellation can be activated in detergent permeabilized Chlamydomonas reinhardtii by the addition of Ca2+ (Sanders, M. A., and J. L. Salisbury, 1989. J. Cell Biol. 108:1751-1760). This suggests that stimuli which induce deflagellation in living cells cause an increase in the intracellular concentration of Ca2+, but this has never been demonstrated. In this paper we report that the wasp venom peptide, mastoparan, and the permeant organic acid, benzoate, activate two different signalling pathways to trigger deflagellation. We have characterized each pathway with respect to: (a) the requirement for extracellular Ca2+; (b) sensitivity to Ca2+ channel blockers; and (c) 45Ca influx. We also report that a new mutant strain of C. reinhardtii, adf-1, is specifically defective in the acid-activated signalling pathway. Both signalling pathways appear normal in another mutant, fa-1, that is defective in the machinery of deflagellation (Lewin, R. and C. Burrascano. 1983. Experientia. 39:1397-1398; Sanders, M. A., and J. L. Salisbury. 1989. J. Cell Biol. 108:1751-1760). We conclude that mastoparan induces the release of an intracellular pool of Ca2+ whereas acid induces an influx of extracellular Ca2+ to activate the machinery of deflagellation.

P049. Cannabinoid-mediated nitric oxide signal transduction in N18TG2 neuronal cells

Nitric Oxide ◽  
2006 ◽  
Vol 14 (4) ◽  
pp. 33
Author(s):  
Derek C. Norford ◽  
Skyla T. Carney ◽  
Vicky I. Arrington ◽  
Jenelle D. Jones ◽  
Michael L. Lloyd ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Signal Transduction ◽  
Neuronal Cells

Excitation, oscillations and wave propagation in a G-protein-based model of signal transduction in Dictyostelium discoideum

1995 ◽  
Vol 349 (1328) ◽  
pp. 179-195 ◽  
Keyword(s):  
Signal Transduction ◽  
G Protein ◽  
Dictyostelium Discoideum ◽  
Spiral Waves ◽  
Experimental Conditions ◽  
Sustained Oscillations ◽  
Cell Biol ◽  
Stimulatory G Protein ◽  
Inhibitory G Protein ◽  
Cellular Slime

In an earlier paper (Tang & Othmer 1994 Math. Biosci 120, 25-76), we developed a G-protein-based model for signal transduction in the cellular slime mould Dictyostelium discoideum and showed that it can account for the results from perfusion experiments done by Devreotes and coworkers (Devreotes et al. 1979 J. Cell. 80, 300-309; Devreotes & Steck 1979 J. Cell Biol. 80, 300-309; Dinauer et al. 1980 J. Cell Biol. 86, 537—561). The primary experimental observables are the amounts of cAMP secreted and the time scale of adaptation in response to various stimuli, and we showed that the predictions of the model agree well with the observations. Adaptation in the model arises from dual receptor-mediated pathways, one of which produces a stimulatory G protein G s and the other of which produces an inhibitory G protein Gt. In this paper we use the model to simulate the suspension experiments of Gerisch & Wick (1975 Biochem. biophys. Res. Commun. 65, 364—370) and the experiments done in cell cultures on Petri dishes (Tomchik & Devreotes 1981 Scien, Wash. 212, 443-446). The model predicts excitation to cAMP stimuli, sustained oscillations, or spiral waves and target patterns, depending on the developmental stage of the cells and experimental conditions. The interaction between different pacemakers is also studied.

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