scholarly journals Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment

2017 ◽  
Vol 216 (6) ◽  
pp. 1623-1639 ◽  
Author(s):  
Philip Auckland ◽  
Nicholas I. Clarke ◽  
Stephen J. Royle ◽  
Andrew D. McAinsh
Keyword(s):  
Bearing Capacity ◽  
Spindle Assembly Checkpoint ◽  
Spindle Assembly ◽  
Live Cells ◽  
Load Bearing Capacity ◽  
Load Bearing ◽  
Naturally Occurring ◽  
Chromosome Congression ◽  
Spindle Microtubules

Kinetochores mediate chromosome congression by either sliding along the lattice of spindle microtubules or forming end-on attachments to their depolymerizing plus-ends. By following the fates of individual kinetochores as they congress in live cells, we reveal that the Ska complex is required for a distinct substep of the depolymerization-coupled pulling mechanism. Ska depletion increases the frequency of naturally occurring, force-dependent P kinetochore detachment events, while being dispensable for the initial biorientation and movement of chromosomes. In unperturbed cells, these release events are followed by reattachment and successful congression, whereas in Ska-depleted cells, detached kinetochores remain in a futile reattachment/detachment cycle that prevents congression. We further find that Ska is progressively loaded onto bioriented kinetochore pairs as they congress. We thus propose a model in which kinetochores mature through Ska complex recruitment and that this is required for improved load-bearing capacity and silencing of the spindle assembly checkpoint.

Assessment of the Load-Bearing Capacity of Bark-Included Junctions in Crataegus monogyna Jacq. in the Presence and Absence of Natural Braces

2020 ◽  
Vol 46 (3) ◽  
pp. 210-227
Author(s):  
Dean Meadows ◽  
Duncan Slater
Keyword(s):  
Bearing Capacity ◽  
Mechanical Performance ◽  
Mechanical Resistance ◽  
Single Specimen ◽  
Load Bearing Capacity ◽  
Load Bearing ◽  
Crataegus Monogyna ◽  
Naturally Occurring ◽  
Mode Of Failure ◽  
Control Branch

Bark-included junctions are frequently encountered defects within the aerial structures of trees. The presence of included bark within a branch junction can substantially reduce the junction’s factor of safety. Recent research has found naturally occurring bracing to be a primary cause of the formation of included bark within branch junctions. This study tested the load-bearing capacity of branch junctions in hawthorn (Crataegus monogyna Jacq.) using rupture tests and compared the mechanical performance of “control” branch junctions, bark-included junctions with the natural bracing retained, and bark-included junctions where we had intentionally removed their natural braces by cutting them out. Substantial variability was observed in the failure kinematics of bark-included branch junctions when their natural braces were retained. The type of natural brace present affected the mode of failure of the branch junctions when pulled apart. A single specimen with fused branches presented the strongest form of natural brace in this study, followed by entwining branches, whereas crossing branches were found to provide the least mechanical resistance. This study provides initial evidence that the type of associated natural brace is an important consideration when an arborist is trying to assess the likely mechanical performance of a bark-included junction within a tree and its likelihood of failure.

Increased load bearing capacity of mechanically joined FRP/metal joints using a pin structured auxiliary joining element

2020 ◽  
Vol 62 (1) ◽  
pp. 55-60
Author(s):  
Per Heyser ◽  
Vadim Sartisson ◽  
Gerson Meschut ◽  
Marcel Droß ◽  
Klaus Dröder
Keyword(s):  
Bearing Capacity ◽  
Load Bearing Capacity ◽  
Load Bearing

Load-Bearing Capacity of Direct Inlay-Retained Fibre-reinforced Composite Fixed Partial Dentures with Different Cross-Sectional Pontic Design

2017 ◽  
Vol 68 (1) ◽  
pp. 94-100
Author(s):  
Oana Tanculescu ◽  
Adrian Doloca ◽  
Raluca Maria Vieriu ◽  
Florentina Mocanu ◽  
Gabriela Ifteni ◽  
...  
Keyword(s):  
Bearing Capacity ◽  
Fracture Pattern ◽  
Load Bearing Capacity ◽  
Cross Sectional ◽  
Load Bearing ◽  
Reinforced Composite ◽  
Polyethylene Fibre

The load-bearing capacity and fracture pattern of direct inlay-retained FRC FDPs with two different cross-sectional designs of the ponticwere tested. The aim of the study was to evaluate a new fibre disposition. Two types of composites, Filtek Bulk Fill Posterior Restorative and Filtek Z250 (3M/ESPE, St. Paul, MN, USA), and one braided polyethylene fibre, Construct (Kerr, USA) were used. The results of the study suggested that the new tested disposition of the fibres prevented in some extend the delamination of the composite on buccal and facial sides of the pontic and increased the load-bearing capacity of the bridges.

scholarly journals The energy absorption behavior of novel composite sandwich structures reinforced with trapezoidal latticed webs

2021 ◽  
Vol 60 (1) ◽  
pp. 503-518
Author(s):  
Juan Han ◽  
Lu Zhu ◽  
Hai Fang ◽  
Jian Wang ◽  
Peng Wu
Keyword(s):  
Bearing Capacity ◽  
Energy Absorption ◽  
Sandwich Structure ◽  
Ultimate Load ◽  
Sandwich Structures ◽  
Elastic Displacement ◽  
Load Bearing Capacity ◽  
Load Bearing ◽  
Composite Sandwich ◽  
Composite Sandwich Structures

Abstract This article proposed an innovative composite sandwich structure reinforced with trapezoidal latticed webs with angles of 45°, 60° and 75°. Four specimens were conducted according to quasi-static compression methods to investigate the compressive behavior of the novel composite structures. The experimental results indicated that the specimen with 45° trapezoidal latticed webs showed the most excellent energy absorption ability, which was about 2.5 times of the structures with vertical latticed webs. Compared to the traditional composite sandwich structure, the elastic displacement and ultimate load-bearing capacity of the specimen with 45° trapezoidal latticed webs were increased by 624.1 and 439.8%, respectively. Numerical analysis of the composite sandwich structures was carried out by using a nonlinear explicit finite element (FE) software ANSYS/LS-DYNA. The influence of the thickness of face sheets, lattice webs and foam density on the elastic ultimate load-bearing capacity, the elastic displacement and initial stiffness was analyzed. This innovative composite bumper device for bridge pier protection against ship collision was simulated to verify its performance. The results showed that the peak impact force of the composite anti-collision device with 45° trapezoidal latticed webs would be reduced by 17.3%, and the time duration will be prolonged by about 31.1%.

scholarly journals Constructing a biomimetic robust bi-layered hydrophilic lubrication coating on surface of silicone elastomer

Friction ◽  
2021 ◽  
Author(s):  
Luyao Gao ◽  
Xiaoduo Zhao ◽  
Shuanhong Ma ◽  
Zhengfeng Ma ◽  
Meirong Cai ◽  
...  
Keyword(s):  
Bearing Capacity ◽  
Composite Layer ◽  
High Load ◽  
Silicone Elastomer ◽  
Aqueous Lubrication ◽  
Low Friction ◽  
Load Bearing Capacity ◽  
Load Bearing ◽  
Lubrication Performance ◽  
Wide Range

AbstractSilicone elastomers-based materials have been extensively involved in the field of biomedical devices, while their use is extremely restricted due to the poor surface lubricity and inherent hydrophobicity. This paper describes a novel strategy for generating a robust layered soft matter lubrication coating on the surface of the polydimethylsiloxane (PDMS) silicone elastomer, by entangling thick polyzwitterionic polyelectrolyte brush of poly (sulfobetaine methacrylate) (PSBMA) into the sub-surface of the initiator-embedded stiff hydrogel coating layer of P(AAm-co-AA-co-HEMA-Br)/Fe, to achieve a unified low friction and high load-bearing properties. Meanwhile, the stiff hydrogel layer with controllable thickness is covalently anchored on the surface of PDMS by adding iron powder to provide catalytic sites through surface catalytically initiated radical polymerization (SCIRP) method and provides high load-bearing capacity, while the topmost brush/hydrogel composite layer is highly effective for aqueous lubrication. Their synergy effects are capable of attaining low friction coefficient (COFs) under wide range of loaded condition in water environment with steel ball as sliding pair. Furthermore, the influence of mechanical modulus of the stiff hydrogel layer on the lubrication performance of layered coating is investigated, for which the COF is the lowest only when the modulus of the stiff hydrogel layer well matches the PDMS substrate. Surprisingly, the COF of the modified PDMS could remain low friction (COF < 0.05) stably after encountering 50,000 sliding cycles under 10 N load. Finally, the surface wear characterizations prove the robustness of the layered lubricating coating. This work provides a new route for engineering lubricious silicon elastomer with low friction, high load-bearing capacity, and considerable durability.

scholarly journals A Modified Pressure–Sinkage Model for Studying the Effect of a Hard Layer in Sandy Loam Soil

2021 ◽  
Vol 11 (12) ◽  
pp. 5499
Author(s):  
Nihal D. Salman ◽  
György Pillinger ◽  
Muammel M. Hanon ◽  
Péter Kiss
Keyword(s):  
Bearing Capacity ◽  
Sandy Loam ◽  
Sandy Loam Soil ◽  
High Fidelity ◽  
Load Bearing Capacity ◽  
Soil Thickness ◽  
Load Bearing ◽  
Hard Layer ◽  
Loam Soil ◽  
New Perspective

The applicability of the typical pressure–sinkage models used to characterize the soil’s bearing properties is limited to homogeneous soils (infinite thickness) that have no hard layer. At a given depth, a hard layer can have a considerable impact on the soil’s load-bearing capacity. It is thus necessary to alter the pressure–sinkage equation by taking this condition into account when assessing the load-bearing capacity. The present paper aims to determine a simple, high-fidelity model, in terms of soil characterization, that can account for the hard layer affection. To assess hard layer affection in this paper, a plate sinkage test (bevameter) was conducted on sandy loam soil. To this end, the soil was prepared by considering three bulk densities and two soil thickness levels at 7–9% moisture content levels. According to the results, this paper put forth a new perspective and related equations for characterizing bearing performance. The sinkage modulus (k) is an intrinsic soil parameter that has a determined unit of N/cm2 and is significant for managing the bearing performance. The results showed that the new modulus sinkage model incorporates the main factor of the rigid layer effect involving high fidelity that the conventional models have failed to account for.

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