scholarly journals Septin pairs, a complex choreography

2011 ◽  
Vol 193 (6) ◽  
pp. 959-961 ◽  
Author(s):  
Helge Ewers
Keyword(s):  
Fluorescence Polarization ◽  
Budding Yeast ◽  
Polarization Microscopy ◽  
Cell Biol ◽  
Bud Neck ◽  
Fluorescence Polarization Microscopy

Septins form a filamentous collar at the mother–bud neck in budding yeast. In cytokinesis, this collar splits into two rings and the septin complexes undergo a dramatic reorientation. Using fluorescence polarization microscopy, DeMay et al. (2011. J. Cell Biol. doi:10.1083/jcb.201012143) now demonstrate that septin complexes assemble as paired filaments in vivo and reveal new insights into septin organization during cytokinesis.

scholarly journals Genetically encoded orientation probes for F-actin for fluorescence polarization microscopy

Microscopy ◽  
2019 ◽  
Vol 68 (5) ◽  
pp. 359-368 ◽  
Author(s):  
Nori Nakai ◽  
Keisuke Sato ◽  
Tomomi Tani ◽  
Kenta Saito ◽  
Fumiya Sato ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Fluorescent Protein ◽  
Cultured Cells ◽  
Optical Resolution ◽  
Cytoskeletal Proteins ◽  
Actin Binding ◽  
Polarization Microscopy ◽  
Fluorescence Polarization Microscopy ◽  
Α Helix

Abstract Fluorescence polarization microscopy, which can visualize both position and orientation of fluorescent molecules, is useful for analyzing architectural dynamics of proteins in vivo, especially that of cytoskeletal proteins such as actin. Fluorescent phalloidin conjugates and SiR-actin can be used as F-actin orientation probes for fluorescence polarization microscopy, but a lack of appropriate methods for their introduction to living specimens especially to tissues, embryos, and whole animals hampers their applications to image the orientation of F-actin. To solve this problem, we have developed genetically encoded F-actin orientation probes for fluorescence polarization microscopy. We rigidly connected circular permutated green fluorescent protein (GFP) to the N-terminal α-helix of actin-binding protein Lifeact or utrophin calponin homology domain (UtrCH), and normal mEGFP to the C-terminal α-helix of UtrCH. After evaluation of ensemble and single particle fluorescence polarization with the instantaneous FluoPolScope, one of the constructs turned out to be suitable for practical usage in live cell imaging. Our new, genetically encoded F-actin orientation probe, which has a similar property of an F-actin probe to conventional GFP-UtrCH, is expected to report the 3D architecture of the actin cytoskeleton with fluorescence polarization microscopy, paving the way for both the single molecular orientation imaging in cultured cells and the sub-optical resolution architectural analysis of F-actin networks analysis of F-actin in various living systems.

scholarly journals Cadherin Organization in Desmosomes Probed using Fluorescence Polarization Microscopy

2020 ◽  
Vol 118 (3) ◽  
pp. 308a-309a
Author(s):  
William F. Dean ◽  
Emily I. Bartle ◽  
Alexa L. Mattheyses
Keyword(s):  
Fluorescence Polarization ◽  
Polarization Microscopy ◽  
Fluorescence Polarization Microscopy

scholarly journals Measuring the Orientation of Single Proteins Interacting with DNA using Fluorescence Polarization Microscopy

2017 ◽  
Vol 112 (3) ◽  
pp. 169a
Author(s):  
Emil Marklund ◽  
Elias Amselem ◽  
Kalle Kipper ◽  
Magnus Johansson ◽  
Sebastian Deindl ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Polarization Microscopy ◽  
Fluorescence Polarization Microscopy

scholarly journals Correction to Monitoring Nanoscale Deformations in a Drawn Polymer Melt with Single-Molecule Fluorescence Polarization Microscopy

ACS Nano ◽  
2016 ◽  
Vol 10 (3) ◽  
pp. 3873-3873
Author(s):  
Stefan Krause ◽  
Martin Neumann ◽  
Melanie Fröbe ◽  
Robert Magerle ◽  
Christian von Borczyskowski
Keyword(s):  
Single Molecule ◽  
Fluorescence Polarization ◽  
Polymer Melt ◽  
Polarization Microscopy ◽  
Single Molecule Fluorescence ◽  
Fluorescence Polarization Microscopy

scholarly journals Super-resolution fluorescence polarization microscopy

2017 ◽  
Vol 11 (01) ◽  
pp. 1730002 ◽  
Author(s):  
Karl Zhanghao ◽  
Juntao Gao ◽  
Dayong Jin ◽  
Xuedian Zhang ◽  
Peng Xi
Keyword(s):  
Fluorescence Polarization ◽  
Super Resolution ◽  
Biological Imaging ◽  
Biological Macromolecules ◽  
Polarization Microscopy ◽  
Dipole Orientation ◽  
Fluorescence Polarization Microscopy ◽  
Microscopy Techniques ◽  
Cellular Organelles

Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules. Several recent super resolution techniques have been applied to fluorescence polarization microscopy, achieving dipole measurement at nanoscale. In this review, we summarize both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging.

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