scholarly journals Circulating tumor cells: approaches to isolation and characterization

2011 ◽  
Vol 192 (3) ◽  
pp. 373-382 ◽  
Author(s):  
Min Yu ◽  
Shannon Stott ◽  
Mehmet Toner ◽  
Shyamala Maheswaran ◽  
Daniel A. Haber
Keyword(s):  
Early Detection ◽  
Disease Progression ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Applications ◽  
Blood Components ◽  
Early Detection Of Disease ◽  
Isolation And Characterization ◽  
Treatment Responses ◽  
Technological Challenge

Circulating tumor cells (CTCs) shed from primary and metastatic cancers are admixed with blood components and are thus rare, making their isolation and characterization a major technological challenge. CTCs hold the key to understanding the biology of metastasis and provide a biomarker to noninvasively measure the evolution of tumor genotypes during treatment and disease progression. Improvements in technologies to yield purer CTC populations amenable to better cellular and molecular characterization will enable a broad range of clinical applications, including early detection of disease and the discovery of biomarkers to predict treatment responses and disease progression.

scholarly journals Covalent chemistry on nanostructured substrates enables noninvasive quantification of gene rearrangements in circulating tumor cells

2019 ◽  
Vol 5 (7) ◽  
pp. eaav9186 ◽  
Author(s):  
Jiantong Dong ◽  
Yu Jen Jan ◽  
Ju Cheng ◽  
Ryan Y. Zhang ◽  
Meng Meng ◽  
...  
Keyword(s):  
Disease Progression ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Chain Reaction ◽  
Biopsy Specimens ◽  
Copy Numbers ◽  
Polymerase Chain ◽  
Treatment Responses ◽  
Rapid Purification ◽  
Digital Polymerase Chain Reaction

Well-preserved mRNA in circulating tumor cells (CTCs) offers an ideal material for conducting molecular profiling of tumors, thereby providing a noninvasive diagnostic solution for guiding treatment intervention and monitoring disease progression. However, it is technically challenging to purify CTCs while retaining high-quality mRNA.Here, we demonstrate a covalent chemistry–based nanostructured silicon substrate (“Click Chip”) for CTC purification that leverages bioorthogonal ligation–mediated CTC capture and disulfide cleavage–driven CTC release. This platform is ideal for CTC mRNA assays because of its efficient, specific, and rapid purification of pooled CTCs, enabling downstream molecular quantification using reverse transcription Droplet Digital polymerase chain reaction. Rearrangements of ALK/ROS1 were quantified using CTC mRNA and matched with those identified in biopsy specimens from 12 patients with late-stage non–small cell lung cancer. Moreover, CTC counts and copy numbers of ALK/ROS1 rearrangements could be used together for evaluating treatment responses and disease progression.

Enrichment and enumeration of circulating tumor cells by efficient depletion of leukocyte fractions

2014 ◽  
Vol 52 (2) ◽  
Author(s):  
Shiyang Wu ◽  
Zhiming Liu ◽  
Suyan Liu ◽  
Li Lin ◽  
Weiwei Yang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Treatment Efficacy ◽  
Epithelial Tumor ◽  
Human Leukocytes ◽  
Isolation And Characterization ◽  
Epithelial Antigens ◽  
Technological Challenge ◽  
Patient Prognosis

AbstractEnumeration and characterization of circulating tumor cells (CTCs) can provide information on patient prognosis and treatment efficacy. However, CTCs are rare, making their isolation a major technological challenge. We developed a technique for enrichment, and subsequent characterization of CTCs based on efficient depletion of human leukocytes.: The technique (CanPatrolThe spiking experiments showed that the average efficacy of leukocytes depletion was 99.98% and the average tumor cells recovery was not lower than 80%. FISH could be used to performThe technique we developed allowed isolation and characterization of circulating epithelial tumor cells that do not express classical epithelial antigens. This potentially leads to a more accurate enumeration of the number of CTCs and is suitable for application to a broad range of cancers.

scholarly journals Technical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169427 ◽  
Author(s):  
Sophie Laget ◽  
Lucile Broncy ◽  
Katia Hormigos ◽  
Dalia M. Dhingra ◽  
Fatima BenMohamed ◽  
...  
Keyword(s):  
Early Detection ◽  
Tumor Cells ◽  
Molecular Characterization ◽  
Circulating Tumor Cells ◽  
Tumor Invasion ◽  
Highly Sensitive

Clinical Applications of Circulating Tumor Cells in Breast Cancer

2019 ◽  
pp. 147-160 ◽  
Author(s):  
Erin F. Cobain ◽  
Costanza Paoletti ◽  
Jeffrey B. Smerage ◽  
Daniel F. Hayes
Keyword(s):  
Breast Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Applications

scholarly journals Circulating tumor cells: silent predictors of metastasis

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1445 ◽  
Author(s):  
LanLan Zhou ◽  
David T. Dicker ◽  
Elizabeth Matthew ◽  
Wafik S. El-Deiry ◽  
R. Katherine Alpaugh
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Disease ◽  
Metastatic Breast ◽  
Prognostic Indicator ◽  
Disease Recurrence ◽  
Curative Intent ◽  
Early Detection Of Disease ◽  
Mere Presence ◽  
Intent To Treat

Circulating tumor cells (CTCs) were added to the arsenal of clinical testing in 2004 for three cancer types: metastatic breast, prostate, and colorectal cancer. CTCs were found to be an independent prognostic indicator of survival for these three diseases. Multiple enrichment/isolation strategies have been developed and numerous assay applications have been performed using both single and pooled captured/enriched CTCs. We have reviewed the isolation techniques and touched on many analyses. The true utility of a CTC is that it acts as a “silent” predictor of metastatic disease. The mere presence of a single CTC is an indication that disease has spread from the primary site. Comments and suggestions have been set forth for CTCs and cell-free DNA to be used as a screening panel for the early detection of disease recurrence and metastatic spread, providing the opportunity for early intervention with curative intent to treat metastatic disease.

scholarly journals Circulating Tumor Cells: Liquid Biopsy for Early Detection of Cancer

2019 ◽  
Vol 25 (1) ◽  
pp. 1-9
Author(s):  
Benjamin Mwesige ◽  
Seung-Gu Yeo ◽  
Byong Chul Yoo
Keyword(s):  
Early Detection ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Early Detection Of Cancer
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