scholarly journals How genes find their way inside the cell nucleus

2007 ◽  
Vol 179 (6) ◽  
pp. 1093-1094 ◽  
Author(s):  
Maria Carmo-Fonseca
Keyword(s):  
Cell Nucleus ◽  
Live Cell Imaging ◽  
Gene Locus ◽  
Recent Progress ◽  
Cell Imaging ◽  
Live Cell ◽  
Nuclear Actin ◽  
First Time

Recent progress in live cell imaging suggests a role for nuclear actin in chromatin movement. In this issue, for the first time, a gene locus moving toward a subnuclear compartment was tracked. Motion of the locus is actin dependent, raising the question of whether chromatin movements are random or directed.

scholarly journals From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19–21, 2006

2006 ◽  
Vol 174 (4) ◽  
pp. 481-484 ◽  
Author(s):  
Yu-li Wang ◽  
Klaus M. Hahn ◽  
Robert F. Murphy ◽  
Alan F. Horwitz
Keyword(s):  
Cell Biology ◽  
Live Cell Imaging ◽  
Recent Progress ◽  
Cell Imaging ◽  
Live Cell ◽  
The Other ◽  
Biological Applications ◽  
Research Fields ◽  
Recent Meeting ◽  
The One

A recent meeting entitled Frontiers in Live Cell Imaging was attended by more than 400 cell biologists, physicists, chemists, mathematicians, and engineers. Unlike typical special topics meetings, which bring together investigators in a defined field primarily to review recent progress, the purpose of this meeting was to promote cross-disciplinary interactions by introducing emerging methods on the one hand and important biological applications on the other. The goal was to turn live cell imaging from a “technique” used in cell biology into a new exploratory science that combines a number of research fields.

scholarly journals Live cell imaging of nuclear actin filaments and heterochromatic repair foci in Drosophila and mouse cells

2019 ◽  
Author(s):  
Colby See ◽  
Deepak Arya ◽  
Emily Lin ◽  
Irene Chiolo
Keyword(s):  
Image Processing ◽  
Dna Sequences ◽  
Actin Filaments ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Temporal Dynamics ◽  
Nuclear Architecture ◽  
Live Cell ◽  
Nuclear Actin ◽  
Mouse Cells

Pericentromeric heterochromatin largely comprises repeated DNA sequences prone to aberrant recombination during double-strand break (DSB) repair. Studies in Drosophila and mouse cells revealed that ‘safe’ homologous recombination (HR) repair of these sequences relies on the relocalization of repair sites to outside the heterochromatin domain before Rad51 recruitment. Relocalization requires a striking network of nuclear actin filaments (F-actin) and myosins generating directed motions. Understanding this pathway requires the ability to detect nuclear actin filaments that are significantly less abundant than cytoplasmic filaments, and to image and track repair sites for long time periods. Here we describe an optimized protocol for live cell imaging of nuclear F-actin in response to IR in Drosophila cells, and for repair focus tracking in mouse cells, including imaging setup, image processing approaches, and analytical methods. We emphasize approaches that can be applied to identify the most effective fluorescent markers for live cell imaging, strategies to minimize photobleaching and phototoxicity with a DeltaVision deconvolution microscope, and image processing and analysis methods using SoftWoRx and Imaris software. These approaches enable a deeper understanding of the spatial and temporal dynamics of heterochromatin repair and have broad applicability in the fields of nuclear architecture, nuclear dynamics, and DNA repair.

scholarly journals Live cell imaging of nuclear actin filaments and heterochromatic repair foci in Drosophila and mouse cells

2019 ◽  
Author(s):  
Colby See ◽  
Deepak Arya ◽  
Emily Lin ◽  
Irene Chiolo
Keyword(s):  
Image Processing ◽  
Dna Sequences ◽  
Actin Filaments ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Temporal Dynamics ◽  
Nuclear Architecture ◽  
Live Cell ◽  
Nuclear Actin ◽  
Mouse Cells

Pericentromeric heterochromatin largely comprises repeated DNA sequences prone to aberrant recombination during double-strand break (DSB) repair. Studies in Drosophila and mouse cells revealed that ‘safe’ homologous recombination (HR) repair of these sequences relies on the relocalization of repair sites to outside the heterochromatin domain before Rad51 recruitment. Relocalization requires a striking network of nuclear actin filaments (F-actin) and myosins generating directed motions. Understanding this pathway requires the ability to detect nuclear actin filaments that are significantly less abundant than cytoplasmic filaments, and to image and track repair sites for long time periods. Here we describe an optimized protocol for live cell imaging of nuclear F-actin in response to IR in Drosophila cells, and for repair focus tracking in mouse cells, including imaging setup, image processing approaches, and analytical methods. We emphasize approaches that can be applied to identify the most effective fluorescent markers for live cell imaging, strategies to minimize photobleaching and phototoxicity with a DeltaVision deconvolution microscope, and image processing and analysis methods using SoftWoRx and Imaris software. These approaches enable a deeper understanding of the spatial and temporal dynamics of heterochromatin repair and have broad applicability in the fields of nuclear architecture, nuclear dynamics, and DNA repair.

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