scholarly journals A functional network involved in the recycling of nucleocytoplasmic pre-60S factors

2006 ◽  
Vol 173 (3) ◽  
pp. 349-360 ◽  
Author(s):  
Alice Lebreton ◽  
Cosmin Saveanu ◽  
Laurence Decourty ◽  
Jean-Christophe Rain ◽  
Alain Jacquier ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Functional Network ◽  
Ribosomal Subunits ◽  
Small Complex ◽  
Key Factor ◽  
Ribosomal Rna Processing ◽  
Cytoplasmic Maturation ◽  
Nucleocytoplasmic Proteins ◽  
Late Stages

Eukaryotic pre-ribosomes go through cytoplasmic maturation steps before entering translation. The nucleocytoplasmic proteins participating in these late stages of maturation are reimported to the nucleus. In this study, we describe a functional network focused on Rei1/Ybr267w, a strictly cytoplasmic pre-60S factor indirectly involved in nuclear 27S pre-ribosomal RNA processing. In the absence of Rei1, the nuclear import of at least three other pre-60S factors is impaired. The accumulation in the cytoplasm of a small complex formed by the association of Arx1 with a novel factor, Alb1/Yjl122w, inhibits the release of the putative antiassociation factor Tif6 from the premature large ribosomal subunits and its recycling to the nucleus. We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation.

scholarly journals Both endonucleolytic and exonucleolytic cleavage mediate ITS1 removal during human ribosomal RNA processing

2013 ◽  
Vol 200 (5) ◽  
pp. 577-588 ◽  
Author(s):  
Katherine E. Sloan ◽  
Sandy Mattijssen ◽  
Simon Lebaron ◽  
David Tollervey ◽  
Ger J.M. Pruijn ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Large Subunit ◽  
Genetic Diseases ◽  
Evolutionary Conservation ◽  
Rrna Processing ◽  
Ribosomal Subunits ◽  
Important Pathway ◽  
Ribosomal Rna Processing ◽  
Insight Into

Human ribosome production is up-regulated during tumorogenesis and is defective in many genetic diseases (ribosomopathies). We have undertaken a detailed analysis of human precursor ribosomal RNA (pre-rRNA) processing because surprisingly little is known about this important pathway. Processing in internal transcribed spacer 1 (ITS1) is a key step that separates the rRNA components of the large and small ribosomal subunits. We report that this was initiated by endonuclease cleavage, which required large subunit biogenesis factors. This was followed by 3′ to 5′ exonucleolytic processing by RRP6 and the exosome, an enzyme complex not previously linked to ITS1 removal. In contrast, RNA interference–mediated knockdown of the endoribonuclease MRP did not result in a clear defect in ITS1 processing. Despite the apparently high evolutionary conservation of the pre-rRNA processing pathway and ribosome synthesis factors, each of these features of human ITS1 processing is distinct from those in budding yeast. These results also provide significant insight into the links between ribosomopathies and ribosome production in human cells.

Adenovirus infection retards ribosomal RNA processing

1989 ◽  
Vol 138 (1) ◽  
pp. 205-207 ◽  
Author(s):  
Susan H. Lawler ◽  
Robert W. Jones ◽  
Brian P. Eliceiri ◽  
George L. Eliceiri
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Adenovirus Infection ◽  
Ribosomal Rna Processing

Developmental expression of fibrillarin and U3 snRNA in Xenopus laevis

Development ◽  
1991 ◽  
Vol 112 (1) ◽  
pp. 317-326
Author(s):  
M. Caizergues-Ferrer ◽  
C. Mathieu ◽  
P. Mariottini ◽  
F. Amalric ◽  
F. Amaldi
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Protein Detection ◽  
Developmental Expression ◽  
Oocyte Growth ◽  
Small Nuclear Ribonucleoprotein ◽  
Hybridization Probes ◽  
Ribosomal Rna Processing ◽  
Protein Components ◽  
Nuclear Ribonucleoprotein

Fibrillarin is one of the protein components that together with U3 snRNA constitute the U3 snRNP, a small nuclear ribonucleoprotein particle involved in ribosomal RNA processing in eucaryotic cells. Using an antifibrillarin antiserum for protein detection and a fibrillarin cDNA and a synthetic oligonucleotide complementary to U3 snRNA as hybridization probes, the expression of these two components has been studied during Xenopus development. Fibrillarin mRNA is accumulated early in oogenesis, like many other messengers, and translated during oocyte growth. Fibrillarin protein is thus progressively accumulated throughout oogenesis to be assembled with U3 snRNA and used for ribosome production in the amplified nucleoli. After fertilization, the amount of U3 snRNA decreases while the maternally accumulated fibrillarin mRNA is maintained and utilized to produce more protein. After the mid-blastula transition, stored fibrillarin is assembled with newly synthesized U3 snRNA and becomes localized in the prenucleolar bodies and reforming nucleoli.

Defect in pre-ribosomal RNA processing is involved in myeloid leukemogenesis

2015 ◽  
Vol 43 (9) ◽  
pp. S78
Author(s):  
Hirotaka Matsui ◽  
Akinori Kanai ◽  
Akiko Nagamachi ◽  
Moe Okuno ◽  
Toshiya Inaba
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Ribosomal Rna Processing

scholarly journals The 18S rRNA dimethylase Dim1p is required for pre-ribosomal RNA processing in yeast.

1995 ◽  
Vol 9 (20) ◽  
pp. 2470-2481 ◽  
Author(s):  
D Lafontaine ◽  
J Vandenhaute ◽  
D Tollervey
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
18S Rrna ◽  
Ribosomal Rna Processing

scholarly journals Maize Urb2 protein is required for kernel development and vegetative growth by affecting pre-ribosomal RNA processing

2018 ◽  
Vol 218 (3) ◽  
pp. 1233-1246 ◽  
Author(s):  
Hongqiu Wang ◽  
Kai Wang ◽  
Qingguo Du ◽  
Yafei Wang ◽  
Zhiyuan Fu ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Vegetative Growth ◽  
Kernel Development ◽  
Ribosomal Rna Processing
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