scholarly journals THE FINE STRUCTURE OF THE URINARY BLADDER OF THE TOAD, BUFO MARINUS

1963 ◽  
Vol 16 (1) ◽  
pp. 53-72 ◽  
Author(s):  
Jae Kwon Choi
Keyword(s):  
Fine Structure ◽  
Urinary Bladder ◽  
Bladder Wall ◽  
Single Layer ◽  
Bufo Marinus ◽  
Cell Junctions ◽  
Basal Region ◽  
Basal Cells ◽  
Migratory Cells ◽  
Toad Bufo

The urinary bladder of the toad (Bufo marinus) was studied with both the light and the electron microscope. The bladder wall consists of epithelium, submucosa, and serosa. In the epithelium, four different cell types were recognized on the basis of their fine structure and staining properties with several different dyes. These four were designated as granular cells, mitochondria-rich cells, mucous cells, and basal cells. In addition, migratory cells of a different type were found in the basal region of the epithelium. The luminal surface of the epithelial cells presents irregular microvilli and is coated by PAS-positive material which has been further investigated by histochemical procedures and radioautography. Included is a description of the fine structural details of cell membranes, cell junctions, and intracellular components. The submucosa consists of a delicate stroma of fibroblasts and collagen fibers and also contains blood and lymph vessels, unmyelinated nerves, migratory cells, and smooth muscle cells. The serosa consists of a single layer of serosal (mesothelial) cells which form an uninterrupted covering of the viscus. Possible pathways of sodium and water transport across the bladder wall are discussed.

THE EFFECTS OF NEUROHYPOPHYSIAL EXTRACTS ON WATER TRANSFER ACROSS THE WALL OF THE ISOLATED URINARY BLADDER OF THE TOAD BUFO MARINUS

1958 ◽  
Vol 17 (3) ◽  
pp. 201-209 ◽  
Author(s):  
P. J. BENTLEY
Keyword(s):  
Urinary Bladder ◽  
Bladder Wall ◽  
Water Transfer ◽  
Passive Movement ◽  
Bufo Marinus ◽  
Metabolic Inhibitors ◽  
Osmotic Gradient ◽  
High Concentrations ◽  
Toad Bufo

SUMMARY 1. An in vitro preparation of the urinary bladder of Bufo marinus is described. 2. Small doses of 'Pituitrin' markedly increase the rate of water transfer across the bladder wall when the solutions inside the bladder are hypotonic. 3. Passive movement is small and increases slightly with increases in the osmotic gradient across the bladder wall. It is unaffected by changes in substrate levels or any of the metabolic inhibitors tested except for cyanide which increases it in some cases. 4. The vasopressor neurohypophysial fraction is more active than the oxytocic one in increasing water transfer across the bladder wall. 5. The increase in water transfer depends on an intact oxygen supply and sufficient glucose or pyruvate. 6. Iodoacetate, malonate, cyanide, 2–4-dinitrophenol, and bubbling 5% CO2+95% O2 through Ringer's solution inhibit the water transfer in response to neurohypophysial extract. 7. Diamox is only an effective inhibitor at very high concentrations. 8. The possible mechanism of the water transfer is discussed.

THE EFFECTS OF IONIC CHANGES ON WATER TRANSFER ACROSS THE ISOLATED URINARY BLADDER OF THE TOAD BUFO MARINUS

1959 ◽  
Vol 18 (4) ◽  
pp. 327-333 ◽  
Author(s):  
P. J. BENTLEY
Keyword(s):  
Urinary Bladder ◽  
Potassium Concentration ◽  
Choline Chloride ◽  
Bladder Wall ◽  
Water Transfer ◽  
Bufo Marinus ◽  
Serosal Side ◽  
Ionic Changes ◽  
Toad Bufo

SUMMARY 1. The effects of ionic changes in the bath fluids on water transfer across an in vitro preparation of the urinary bladder of the toad Bufo marinus were investigated. 2. Calcium was necessary to maintain the normal low permeability of the bladder to water in the absence of Pituitrin. Magnesium, strontium and manganese but not barium could substitute for calcium. 3. With low concentration of calcium there was a reduction in the water transfer across the bladder wall in response to Pituitrin and no other divalent ion could substitute for calcium. 4. Exclusion of potassium from the serosal side of the bladder reduced the water transfer in the presence of Pituitrin. Increase in potassium concentration above normal levels also inhibited the response to hormone. When no Pituitrin was present there was no change in water loss from the bladder with alterations in potassium concentration. Absence of potassium from the epithelial side of the bladder had no effect whether Pituitrin was present or not. 5. The presence of sodium on the epithelial side increased the water transfer in response to Pituitrin and neither lithium, choline nor potassium could substitute for it. Replacement of 50% of the sodium chloride on the serosal side of the bladder by choline chloride decreased the water transfer in response to Pituitrin.

Fine structure of the photosensitive iris of the toad,Bufo marinus

1986 ◽  
Vol 188 (2) ◽  
pp. 225-238 ◽  
Author(s):  
L. Rubin ◽  
P. Eller ◽  
J. Nolte
Keyword(s):  
Fine Structure ◽  
Bufo Marinus ◽  
Toad Bufo

DIRECTIONAL DIFFERENCES IN THE PERMEABILITY TO WATER OF THE ISOLATED URINARY BLADDER OF THE TOAD, BUFO MARINUS

1961 ◽  
Vol 22 (1) ◽  
pp. 95-100 ◽  
Author(s):  
P. J. BENTLEY
Keyword(s):  
Urinary Bladder ◽  
Electric Current ◽  
Opposite Direction ◽  
Water Movement ◽  
Water Transfer ◽  
Bufo Marinus ◽  
Osmotic Gradient ◽  
Serosal Side ◽  
Toad Bufo

SUMMARY 1. If the osmotic gradient is favourable vasopressin increases the rate of water movement across the bladder of the toad from the serosal to the epithelial side, which is the opposite direction to that seen physiologically. 2. Water transfer down an osmotic gradient is 1·8 times more rapid towards the serosal than towards the epithelial side. Vasopressin increases this difference so that water is moving 4·9 times as rapidly to the serosal side. Iodoacetate reduces this effect of vasopressin. 3. If water is moving down an osmotic gradient towards the anode, a higher electric current increases the water movement in the presence, but not in the absence, of vasopressin. If water movement is taking place towards the cathode an increased current has no effect. 4. With vasopressin present, absence of sodium on the epithelial side of the bladder reduces water transfer down an osmotic gradient towards the serosal side, but has no effect on water movement down an osmotic gradient in the opposite direction.

Seasonal Changes in the Fine Structure of the Basal Granular Cells of the Bat Thyroid

1967 ◽  
Vol 2 (3) ◽  
pp. 401-410
Author(s):  
E. A. NUNEZ ◽  
R. P. GOULD ◽  
D. W. HAMILTON ◽  
J. S. HAYWARD ◽  
S. J. HOLT
Keyword(s):  
Fine Structure ◽  
Seasonal Changes ◽  
Follicular Epithelium ◽  
Thyroid Cell ◽  
Basal Region ◽  
Basal Cells ◽  
Uniform Size ◽  
Thyroid Cells ◽  
Cytoplasmic Matrix ◽  
Dense Granules

The fine structure of the thyroid gland of non-hibernating, hibernating, and intermittently aroused hibernating bats was examined. It was found that in addition to the ordinary follicular cell, another widespread thyroid cell type is present in all bats examined. This cell is situated in the basal region of the thyroid follicle and is characterized by a cytoplasm full of secretory-like granules. In the basal cells of bats captured in April and June the granules consist of an extremely dense core and are of a uniform size averaging from 0.1-0.5 µ in diameter. In bats caught in August the solid dense granules vary greatly in size and large granules of diameters from 2 to 5 µ are common. These large granules are often found concentrated in groups in the most basal region of the follicular epithelium. Hibernating bats are characterized by partly or totally degranulated basal thyroid cells. The cytoplasmic granules in the partly degranulated cell vary greatly in appearance, ranging from solid dense granules to empty vesicles. In totally degranulated basal cells, empty vesicles fill the cytoplasmic matrix. The granular endoplasmic reticulum of the basal thyroid cell also shows seasonal changes, while the Golgi complex remains a well-developed organelle throughout the year. These observations suggest that the thyroid basal granular cell is involved in secretory activities; its possible functional role is discussed.

Histopathological Study of Using Single-layer Simple Continuous and Continuous Lembert Suture Patterns for Systotomy Closure in Rabbits

2020 ◽  
Vol 13 (1) ◽  
pp. 12-19
Author(s):  
Nadia AL-Falahi ◽  
Ali Atiyah
Keyword(s):  
Urinary Bladder ◽  
Bladder Wall ◽  
Single Layer ◽  
Dorsal Side ◽  
Histological Evaluation ◽  
Histopathological Study ◽  
Microscopic Evaluation ◽  
Post Operation ◽  
Macroscopic Observation ◽  
Group B

Two techniques of single-layer closure were studied experimentally by created cystotomy in six male rabbits, they separated randomly into two equal groups (A and B). In animals of either groups, cystotomy was initiated experimentally on the dorsal side of the urinary bladder wall through standard midline ventral laparotomy. In group A, the cystotomy wound was closed by single-layer, full thickness, simple continuous pattern, while in group B, the closure was accomplished by single-layer continuous Lambert pattern, applying polydioxanone PDS (3/0) in the two groups. The effectiveness of cystotomy wound healing and a urine-firm seal of the urinary bladder were examined at days 3, 7 and 14 post-operatively by gross changes. In both groups, the macroscopic observation revealed thickening of the bladder wall with adhesion in both groups, the microscopic evaluation showed optimum healing at the site of cystotomy wound sealing on the 14th day post operation despite a slight to moderate adhesions were noted in both groups. The histological evaluation of the urinary bladder biopsies indicated regular epithelization of the mucosal coat and normal arrangement of the muscularis and serosal layers at the location of cystotomy closure were evident in animals of both groups on the 14th day post operation.

Active phosphate transport across the urinary bladder of the toad,Bufo marinus

1977 ◽  
Vol 32 (1) ◽  
pp. 291-299 ◽  
Author(s):  
Billy B. Sellers ◽  
Julia A. Hall ◽  
Carol W. Both ◽  
Stanley A. Mendoza
Keyword(s):  
Urinary Bladder ◽  
Phosphate Transport ◽  
Bufo Marinus ◽  
Toad Bufo

Somatostatin: occurrence in urinary bladder epithelium and renal tubules of the toad, Bufo marinus

Science ◽  
1980 ◽  
Vol 210 (4470) ◽  
pp. 644-646 ◽  
Author(s):  
J. Bolaffi ◽  
S Reichlin ◽  
D. Goodman ◽  
J. Forrest
Keyword(s):  
Urinary Bladder ◽  
Bufo Marinus ◽  
Renal Tubules ◽  
Bladder Epithelium ◽  
Toad Bufo
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