scholarly journals PANETH CELL GRANULE OF MOUSE INTESTINE

1962 ◽  
Vol 15 (1) ◽  
pp. 136-139 ◽  
Author(s):  
H. M. Selzman ◽  
Robert A. Liebelt
Keyword(s):  
Paneth Cell ◽  
Cell Granule ◽  
Mouse Intestine

scholarly journals Paneth cell granule depletion in the human small intestine under infective and nutritional stress

2004 ◽  
Vol 135 (2) ◽  
pp. 303-309 ◽  
Author(s):  
P. KELLY ◽  
R. FEAKINS ◽  
P. DOMIZIO ◽  
J. MURPHY ◽  
C. BEVINS ◽  
...  
Keyword(s):  
Small Intestine ◽  
Paneth Cell ◽  
Nutritional Stress ◽  
Cell Granule ◽  
Human Small Intestine

Abnormal Paneth cell granule dissolution and compromised resistance to bacterial colonization in the intestine of CF mice

2004 ◽  
Vol 286 (6) ◽  
pp. G1050-G1058 ◽  
Author(s):  
Lane L. Clarke ◽  
Lara R. Gawenis ◽  
Emily M. Bradford ◽  
Louise M. Judd ◽  
Kathryn T. Boyle ◽  
...  
Keyword(s):  
Northern Blot Analysis ◽  
Clinical Symptoms ◽  
Bacterial Colonization ◽  
Paneth Cell ◽  
Light And Electron Microscopy ◽  
Paneth Cells ◽  
Real Time Quantitative Pcr ◽  
Granule Morphology ◽  
Mouse Intestine ◽  
Osmotic Laxative

Paneth cells of intestinal crypts contribute to host defense by producing antimicrobial peptides that are packaged as granules for secretion into the crypt lumen. Here, we provide evidence using light and electron microscopy that postsecretory Paneth cell granules undergo limited dissolution and accumulate within the intestinal crypts of cystic fibrosis (CF) mice. On the basis of this finding, we evaluated bacterial colonization and expression of two major constituents of Paneth cells, i.e., α-defensins (cryptdins) and lysozyme, in CF murine intestine. Paneth cell granules accumulated in intestinal crypt lumens in both untreated CF mice with impending intestinal obstruction and in CF mice treated with an osmotic laxative that prevented overt clinical symptoms and mucus accretion. Ultrastructure studies indicated little change in granule morphology within mucus casts, whereas granules in laxative-treated mice appear to undergo limited dissolution. Protein extracts from CF intestine had increased levels of processed cryptdins compared with those from wild-type (WT) littermates. Nonetheless, colonization with aerobic bacteria species was not diminished in the CF intestine and oral challenge with a cryptdin-sensitive enteric pathogen, Salmonella typhimurium, resulted in greater colonization of CF compared with WT intestine. Modest downregulation of cryptdin and lysozyme mRNA in CF intestine was shown by microarray analysis, real-time quantitative PCR, and Northern blot analysis. Based on these findings, we conclude that antimicrobial peptide activity in CF mouse intestine is compromised by inadequate dissolution of Paneth cell granules within the crypt lumens.

Localization of lysozyme activity in a Paneth cell granule fraction

1967 ◽  
Vol 139 (1) ◽  
pp. 204-207 ◽  
Author(s):  
Raf J. Deckx ◽  
Gaston R. Vantrappen ◽  
Monique M. Parein
Keyword(s):  
Paneth Cell ◽  
Lysozyme Activity ◽  
Cell Granule ◽  
Granule Fraction

scholarly journals Detection of CD1 mRNA in Paneth cells of the mouse intestine by in situ hybridization.

1992 ◽  
Vol 40 (10) ◽  
pp. 1527-1534 ◽  
Author(s):  
J Lacasse ◽  
L H Martin
Keyword(s):  
In Situ Hybridization ◽  
Cell Biology ◽  
Paneth Cell ◽  
Intestinal Flora ◽  
Intraepithelial Lymphocytes ◽  
Paneth Cells ◽  
Peripheral T Cells ◽  
Mouse Intestine ◽  
Cluster Of Differentiation

Cluster of differentiation 1 (CD1) antigens are a family of non-MHC but Class I-like molecules that have been identified in humans and rodents. Although their function(s) remains unknown, it has been proposed that CD1 may present antigens to specific subsets of peripheral T-cells. We now provide evidence in support of this hypothesis through the demonstration by in situ hybridization that Paneth cells of the mouse intestine express CD1 mRNA. These cells are thought to be involved in the immunological regulation of intestinal flora and could accomplish this task through interactions with intestinal intraepithelial lymphocytes. The expression and localization of CD1 mRNA was confirmed by both autoradiographic and non-isotopic techniques. The relevance of these results to CD1 function as well as to Paneth cell biology is discussed.

scholarly journals Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuki Yokoi ◽  
Kiminori Nakamura ◽  
Tsukasa Yoneda ◽  
Mani Kikuchi ◽  
Rina Sugimoto ◽  
...  
Keyword(s):  
Paneth Cell ◽  
Cell Granule

Human defensin-5 (HD-5) is processed upon paneth cell granule secretion, and its expression is induced in inflammatory bowel disease (IBD)

2000 ◽  
Vol 118 (4) ◽  
pp. A814
Author(s):  
Robert N. Cunliffe ◽  
Felicity R. Rose ◽  
John Keyte ◽  
Lee Abberley ◽  
Weng C. Chan ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Paneth Cell ◽  
Cell Granule ◽  
Inflammatory Bowel ◽  
Granule Secretion ◽  
Human Defensin 5

Cryptdin gene expression in developing mouse small intestine

1997 ◽  
Vol 272 (1) ◽  
pp. G197-G206 ◽  
Author(s):  
D. Darmoul ◽  
D. Brown ◽  
M. E. Selsted ◽  
A. J. Ouellette
Keyword(s):  
Paneth Cell ◽  
Paneth Cells ◽  
Intestinal Lumen ◽  
Intestinal Epithelial ◽  
Rt Pcr ◽  
Adult Mice ◽  
Mucosal Surfaces ◽  
Mouse Small Intestine ◽  
Pcr Products ◽  
Mouse Intestine

In rodents, the four intestinal epithelial cell lineages differentiate and become morphologically distinct during the first 2-3 postnatal wk. In studies reported here, reverse transcriptase-polymerase chain reaction (RT-PCR)-based assays detected Paneth cell defensin mRNAs in intestinal RNA from 1-day-old (P1) mice before crypt formation and maturation of the epithelium. Analysis of these defensin-coding RT-PCR products from P1 mice showed that 69% of clones sequenced coded for cryptdin-6, suggesting that it is the most abundant enteric defensin mRNA in the newborn. Paneth cell mRNAs, including cryptdins-4 and -5, lysozyme, matrilysin, and defensin-related sequences, also were detected in RNA from P1 mouse intestine. Unlike adult mice, where only Paneth cells are immunopositive for cryptdin, cryptdin-containing cells were distributed throughout the newborn intestinal epithelium and not in association with rudimentary crypts. Cryptdin immunoreactivity in the P1 mouse intestine was specific for intracellular granule contents, and immunofluorescent detection of cryptdins on mucosal surfaces suggested that the peptides are released into the intestinal lumen in P1 mice Defensin secretion may contribute to innate immunity of the neonatal intestine before the presence of distinguishable Paneth cells.

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