scholarly journals THE IN VIVO REUTILIZATION OF LYMPHOCYTIC AND SARCOMA DNA BY CELLS GROWING IN THE PERITONEAL CAVITY

1962 ◽  
Vol 13 (2) ◽  
pp. 205-216 ◽  
Author(s):  
William O. Rieke
Keyword(s):  
Peritoneal Cavity ◽  
Mononuclear Cells ◽  
Tritiated Thymidine ◽  
Ascites Tumor ◽  
Diffusion Chambers ◽  
Transplanted Tumor ◽  
Tumor Bearing ◽  
Sarcoma Cells ◽  
Cells Cultured

An ascites tumor, Sarcoma I, was transplanted to isologous and homologous mice which had been labeled with tritiated thymidine from 1 to 24 hours previously. Radioautographic preparations revealed labeled host lymphocytes emerging to mingle with the transplanted tumor and the subsequent appearance of nuclear radioactivity in the sarcoma. Sarcoma cells cultured subcutaneously or in Millipore diffusion chambers in previously labeled mice did not demonstrate significant radioactivity. Transplantation of washed, H3-thymidine-labeled lymphocytes to non-radioactive, sarcoma-bearing mice was followed by the gradual appearance of nuclear radioactivity in the sarcoma. The label in the sarcoma was entirely removed by deoxyribonuclease but not by ribonuclease treatment prior to radioautography. Intraperitoneal injections of purified, H3-thymidine-labeled sarcoma or lymphoid DNA in normal or tumor-bearing mice were followed by radioactivity appearing in sarcoma or normal peritoneal mononuclear cells. It was concluded that reutilization of DNA and its metabolites may occur in vivo, and the conditions under which reutilization may be detected are discussed.

Immunomodulation by Soluble Factors from Tumor Cells Cultured in vivo in Diffusion Chambers

Tumor Biology ◽  
1994 ◽  
Vol 15 (3) ◽  
pp. 160-165 ◽  
Author(s):  
Slobodanka Klein ◽  
Maria Adela Jasnis ◽  
Miriam Diament ◽  
Lilia Davel ◽  
Julio Aguirre ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Soluble Factors ◽  
Diffusion Chambers ◽  
Cells Cultured

Effect of Garlic, Chinese Medicinal Drugs and Amino Acids on Growth of Erlich Ascites Tumor Cells in Mice

1983 ◽  
Vol 11 (01n04) ◽  
pp. 69-73 ◽  
Author(s):  
Y.M. Choy ◽  
T.T. Kwok ◽  
K.P. Fung ◽  
C.Y. Lee
Keyword(s):  
Amino Acids ◽  
Tumor Cells ◽  
Peritoneal Cavity ◽  
Growth And Development ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Tumor Bearing ◽  
Ehrlich Ascites ◽  
Medicinal Drugs ◽  
Growth Of Tumor

A number of food materials or drugs have been screened for the effect on the growth and development of transplantable Ehrlich ascites tumor cells. Growth of tumor-bearing mice was significantly inhibited by feeding garlic as well as some amino acids. These materials significantly reduced the total number of free tumor cells growing in the peritoneal cavity of mice and prolonged significantly the length of time for 50% death of tumor-bearing mice.

Vitamin D modulates the expression of HLA-DR and CD38 after in vitro activation of T-cells

2017 ◽  
Vol 29 (3) ◽  
Author(s):  
Simon Villegas-Ospina ◽  
Wbeimar Aguilar-Jimenez ◽  
Sandra M. Gonzalez ◽  
María T. Rugeles
Keyword(s):  
Vitamin D ◽  
Flow Cytometry ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Activation Markers ◽  
Hla Dr ◽  
In Vitro Activation ◽  
Cells Cultured

AbstractObjective:Vitamin D (VitD) is an anti-inflammatory hormone; however, some evidence shows that VitD may induce the expression of activation markers, such as CD38 and HLA-DR. We explored its effect on the expression of these markers on CD4Materials and methods:CD38 and HLA-DR expression was measured by flow cytometry in PHA/IL-2-activated mononuclear cells cultured under VitD precursors: three cholecalciferol (10Results:Cholecalciferol at 10Conclusion:Although no significant correlations were observed in vivo in healthy subjects, VitD treatment in vitro modulated immune activation by increasing the expression of CD38 and decreasing the proliferation of HLA-DR

scholarly journals THE EFFECT OF SKIN HOMOGRAFT REJECTION ON RECIPIENT AND DONOR MIXED LEUKOCYTE CULTURES

1965 ◽  
Vol 122 (4) ◽  
pp. 651-664 ◽  
Author(s):  
Joost J. Oppenheim ◽  
Jacqueline Whang ◽  
Emil Frei
Keyword(s):  
Mononuclear Cells ◽  
Tritiated Thymidine ◽  
White Blood Cells ◽  
Calf Serum ◽  
Lymphocyte Transformation ◽  
Humoral Factors ◽  
Unstimulated Control ◽  
Graft Donor

The lymphocyte proliferation in repeatedly studied mixed leukocyte cultures of peripheral white blood cells from a skin graft donor and 2 recipients was significantly increased at the time of graft rejection. This was determined from the increased proportions of mononuclear cells labeling with tritiated thymidine, increased mitotic indices, and the appearance of increased numbers of transformed lymphocytes after rejection of 1st and 2nd skin grafts. The temporarily enhanced response occurred sooner and was of shorter duration after the second than after the first graft, but was quantitatively similar each time. The cell proliferation in the mixed leukocyte cultures of the two recipients was similarly affected by the homograft rejections. The cultures containing three cell populations usually manifested a greater lymphocyte response than corresponding cultures of leukocytes from only two unrelated subjects. An increase in the ratio of female recipient to male graft donor metaphases in the cultures at the time of enhanced lymphocyte transformation indicated that proliferation of the graft recipient lymphocytes was responsible for the above findings. Unmixed, unstimulated control cultures grown in autologous, the other subjects plasma, or heterologous calf serum failed to support significant lymphocyte transformation. The role of humoral factors and relationship of the in vitro cellular responses to the in vivo homograft reaction are discussed.

scholarly journals Radioautographic Studies of Bone Marrow Lymphocytes in Vivo and in Diffusion Chamber Cultures

Blood ◽  
1964 ◽  
Vol 23 (1) ◽  
pp. 1-17 ◽  
Author(s):  
D. G. OSMOND ◽  
N. B. EVERETT
Keyword(s):  
Bone Marrow ◽  
Large Scale ◽  
Bone Marrow Cells ◽  
Tritiated Thymidine ◽  
Diffusion Chamber ◽  
Blood Stream ◽  
Turnover Time ◽  
Diffusion Chambers ◽  
Transitional Cells

Abstract Radioautography with tritiated thymidine has been utilized to examine the turnover rate and origin of small lymphocytes in the bone marrow of the guinea-pig. Very few marrow lymphocytes were initially labeled by a single injection of tritiated thymidine, but thereafter the number of labeled lymphocytes rapidly increased to high maximum levels at 3 days. Analysis of the labeling curves and grain counts indicates that the population of marrow lymphocytes is maintained in a dynamic steady state with an average turnover time of 3 days or less. Suspensions of bone marrow cells were isolated from the circulation within intraperitoneal diffusion chambers after short-term labeling with tritiated thymidine in vivo. Although very few small lymphocytes were labeled when introduced into the diffusion chambers, a considerable percentage became labeled during the subsequent culture period. Tritiated thymidine was also administered intravenously whilst excluded from one hind limb by the application of an occlusive compression bandage for 20 minutes. Very few labeled small lymphocytes were found after 72 hours in the tibial marrow of the initially occluded limb, whereas the normal high percentage was labeled in the control tibial marrow. These experiments do not demonstrate any large-scale influx of small lymphocytes from the blood stream into the marrow parenchyma. They suggest that newly formed small lymphocytes appear in the marrow as a result of the division of locally situated precursor cells, but the mechanism of intramedullary lymphocytopoiesis is uncertain. "Transitional" cells, intermediate in morphology between blast cells and small lymphocytes, synthesize DNA and are actively proliferative, but they do not appear to account fully for the rate of lymphocyte production. Certain large, undifferentiated labeled cells appeared in the bone marrow as a result of hematogenous migration. Some implications of these findings are discussed.

In Vivo and in Vitro Methylcholanthrene Carcinogenesis in Diffusion Chambers

1972 ◽  
Vol 58 (5) ◽  
pp. 327-334
Author(s):  
Giorgio Parmiani ◽  
Giusi Carbone ◽  
Richmond T. Prehn
Keyword(s):  
Peritoneal Cavity ◽  
Cultured Cells ◽  
Culture Period ◽  
Diffusion Chambers ◽  
Adult Mice ◽  
Induced Tumors ◽  
Murine Fibroblasts ◽  
Tumor Outgrowth

Two-compartment diffusion chambers containing murine fibroblasts and a 5% (150 μg) or 1% (30 μg) MCA-discs were placed either in the peritoneal cavity of syngeneic adult mice or incubated in vitro. After 4–28 weeks of culture, groups of 5–6 DC's were recovered at 4 weeks intervals, the MCA discarded, and the contents individually implanted into immunodepressed syngeneic mice. The carcinogen displayed a strong cytotoxicity in vitro but not in vivo with both doses. On the whole, when 5% MCA was applied sarcomas appeared in 22/23 mice implanted with cells of the in vivo DC cultures and in 11/16 mice which received the in vitro cultured cells; with the lower dose, the in vivo induced tumors were 19/22 and the in vitro ones 6/12. In all groups the tumor outgrowth into the assay animals was inversely correlated with the time of exposure to MCA during the culture period.

scholarly journals The Cultivation of Mouse Bone Marrow in Vivo

Blood ◽  
1959 ◽  
Vol 14 (9) ◽  
pp. 1040-1046 ◽  
Author(s):  
IRWIN BERMAN ◽  
HENRY S. KAPLAN
Keyword(s):  
Bone Marrow ◽  
Peritoneal Cavity ◽  
Normal Mouse ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Considerable Time ◽  
Diffusion Chambers ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

Abstract The cultivation of normal mouse bone marrow cells in diffusion chambers implanted into the peritoneal cavity of mice has been described. Mouse bone marrow cells cultivated by this method continue to undergo differentiation and maintain their morphologic identity for a considerable time.

Molecular Characterization of Gene Expression Changes in ROS 17/2.8 Cells Cultured in Diffusion Chambers In Vivo

1999 ◽  
Vol 65 (2) ◽  
pp. 133-138 ◽  
Author(s):  
J. E. Onyia ◽  
L. V. Hale ◽  
R. R. Miles ◽  
R. L. Cain ◽  
Y. Tu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Characterization ◽  
Diffusion Chambers ◽  
Cells Cultured
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