scholarly journals Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm [published erratum appears in J Cell Biol 1991 Aug;114(4):following 863]

1991 ◽  
Vol 113 (4) ◽  
pp. 817-833 ◽  
Author(s):  
B. D. Wright
Keyword(s):  
Subcellular Localization ◽  
Sea Urchin ◽  
Heavy Chain ◽  
Mitotic Apparatus ◽  
Cell Biol ◽  
Kinesin Heavy Chain

scholarly journals Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection.

1993 ◽  
Vol 123 (3) ◽  
pp. 681-689 ◽  
Author(s):  
B D Wright ◽  
M Terasaki ◽  
J M Scholey
Keyword(s):  
Cell Division ◽  
Sea Urchin ◽  
Heavy Chain ◽  
Mammalian Cells ◽  
Embryonic Cell ◽  
Mitotic Apparatus ◽  
Specific Antibodies ◽  
Sea Urchin Embryos ◽  
Mitotic Figures

Previous studies suggest that kinesin heavy chain (KHC) is associated with ER-derived membranes that accumulate in the mitotic apparatus in cells of early sea urchin embryos (Wright, B. D., J. H. Henson, K. P. Wedaman, P. J. Willy, J. N. Morand, and J. M. Scholey. 1991. J. Cell Biol. 113:817-833). Here, we report that the microinjection of KHC-specific antibodies into these cells has no effect on mitosis or ER membrane organization, even though one such antibody, SUK4, blocks kinesin-driven motility in vitro and in mammalian cells. Microinjected SUK4 was localized to early mitotic figures, suggesting that it is able to access kinesin in spindles. In contrast to KHC-specific antibodies, two antibodies that react with kinesin-like proteins (KLPs), namely CHO1 and HD, disrupted mitosis and prevented subsequent cell division. CHO1 is thought to exert this effect by blocking the activity of a 110-kD KLP. The relevant target of HD, which was raised against the KHC motor domain, is unknown; HD may disrupt mitosis by interfering with an essential spindle KLP but not with KHC itself, as preabsorption of HD with KHC did not alter its ability to block mitosis. These data indicate that some KLPs have essential mitotic functions in early sea urchin embryos but KHC itself does not.

scholarly journals Resistance is futile: Centering forces yield for asymmetric cell division

2019 ◽  
Vol 218 (3) ◽  
pp. 727-728
Author(s):  
Joshua Alper ◽  
Marija Zanic
Keyword(s):  
Cell Division ◽  
Sea Urchin ◽  
Asymmetric Cell Division ◽  
Asymmetric Division ◽  
Magnetic Tweezers ◽  
Time Dependent ◽  
Mitotic Apparatus ◽  
Cell Biol

Asymmetric cell division relies on microtubule-based forces to asymmetrically position the mitotic apparatus. In this issue, Sallé et al. (2019. J. Cell Biol. https://doi.org/10.1083/jcb.201807102) use magnetic tweezers to induce asymmetric division in sea urchin zygotes, demonstrating that asymmetry could arise from a time-dependent weakening of centering forces.

scholarly journals A Quantitative Description of Protoplasmic Movement During Cleavage in the Sea-Urchin Egg

1958 ◽  
Vol 35 (2) ◽  
pp. 407-424
Author(s):  
Y. HIRAMOTO
Keyword(s):  
Cell Division ◽  
Sea Urchin ◽  
Quantitative Description ◽  
Motive Force ◽  
Mitotic Apparatus ◽  
Band Theory ◽  
Cytoplasmic Granules ◽  
Carbon Particles ◽  
Sea Urchin Egg ◽  
Protoplasmic Movement

1. Protoplasmic movements during cleavage in the eggs of the heart-urchin Clypeaster japonicus have been followed by tracing the movements of cytoplasmic granules and of carbon particles adhering to the surface. 2. These movements are quantitatively described in normal eggs and in eggs whose mitotic apparatus has been destroyed by colchicine. 3. The results obtained are qualitatively similar to those obtained by Spek and by Dan and his collaborators. 4. Endoplasmic movement and changes in the length and shape of the astral rays are readily explained by the contracting-ring (band) theory. 5. The location of the motive force of cell division is discussed.

Molecular Basis of Dystrobrevin Interaction with Kinesin Heavy Chain: Structural Determinants of their Binding

2005 ◽  
Vol 354 (4) ◽  
pp. 872-882 ◽  
Author(s):  
Marina Ceccarini ◽  
Paola Torreri ◽  
Dario Giuseppe Lombardi ◽  
Gianfranco Macchia ◽  
Pompeo Macioce ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Molecular Basis ◽  
Structural Determinants ◽  
Kinesin Heavy Chain

IDENTIFICATION AND PROPERTIES OF RAT BRAIN KINESIN HEAVY CHAIN ASSOCIATED WITH MITOCHONDRIA

1991 ◽  
Vol 73 (2-3) ◽  
pp. 18a-18a ◽  
Author(s):  
Abdeljelil Jellali ◽  
Irina Surgucheva ◽  
Vera Jancsik ◽  
Dominique Filliol ◽  
Alvaro Rendon
Keyword(s):  
Rat Brain ◽  
Heavy Chain ◽  
Kinesin Heavy Chain
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