scholarly journals A retinoic acid responsive gene MK found in the teratocarcinoma system is expressed in spatially and temporally controlled manner during mouse embryogenesis.

1990 ◽  
Vol 110 (3) ◽  
pp. 607-616 ◽  
Author(s):  
K Kadomatsu ◽  
R P Huang ◽  
T Suganuma ◽  
F Murata ◽  
T Muramatsu
Keyword(s):  
Retinoic Acid ◽  
Embryonal Carcinoma ◽  
Anterior Lobe ◽  
Carcinoma Cells ◽  
Embryonic Induction ◽  
Mouse Embryogenesis ◽  
Epithelial Tissues ◽  
Lower Jaw ◽  
The Brain

A newly identified gene MK is transiently expressed in early stages of retinoic acid-induced differentiation of embryonal carcinoma cells (Kadomatsu, K., M. Tomomura, and T. Muramatsu, 1988. Biochem. Biophys. Res. Commun. 151:1312-1318). MK gene has been predicted to code a polypeptide that is rich in basic amino acids and cysteine and is not related to any other peptides so far reported. In the present study, we investigated MK expression during mouse embryogenesis by in situ hybridization. The MK transcript was detected all over the embryo proper of the 7-d embryo, while it was not detectable in the 5-d embryo. The ubiquitous expression continued in the 9-d embryo proper. On the 11th-13th d of gestation, the sites where MK gene was intensely expressed became progressively restricted; these sites were the brain ectoderm around the lens and brain ventricles, the anterior lobe of the pituitary gland, the upper and lower jaw, the caudal sclerotomic half of vertebral column, the limbs, the stomach, and the epithelial tissues of the lung, the pancreas, the small intestine, and the metanephros. These areas include the region where secondary embryonic induction is prominent. In the 15-d embryo, only the kidney expressed MK significantly. These data suggest that MK gene plays a fundamental role in the differentiation of a wide variety of cells; MK gene may also play some specific roles in generation of epithelial tissues, and remodeling of mesoderm.

Dickkopf-3 alters the morphological response to retinoic acid during neuronal differentiation of human embryonal carcinoma cells

2014 ◽  
Vol 74 (12) ◽  
pp. 1243-1254 ◽  
Author(s):  
Rocío Jiménez Alfonso ◽  
Irantzu Gorroño-Etxebarria ◽  
Miriam Rabano ◽  
Maria dM. Vivanco ◽  
Robert Kypta
Keyword(s):  
Retinoic Acid ◽  
Neuronal Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Morphological Response

Expression of p53 during mouse embryogenesis

Development ◽  
1991 ◽  
Vol 113 (3) ◽  
pp. 857-865 ◽  
Author(s):  
P. Schmid ◽  
A. Lorenz ◽  
H. Hameister ◽  
M. Montenarh
Keyword(s):  
Gene Expression ◽  
Cellular Differentiation ◽  
Cellular Proliferation ◽  
In Situ Hybridisation ◽  
Terminal Differentiation ◽  
Mouse Embryogenesis ◽  
Differentiated Cells ◽  
The Brain ◽  
P53 Mrna

By in situ hybridisation we have examined the expression of p53 during mouse embryogenesis from day 8.5 to day 18.5 post coitum (p.c.). High levels of p53 mRNA were detected in all cells of the day 8.5 p.c. and 10.5 p.c. mouse embryo. However, at later stages of development, expression became more pronounced during differentiation of specific tissues e.g. of the brain, liver, lung, thymus, intestine, salivary gland and kidney. In cells undergoing terminal differentiation, the level of p53 mRNA declined strongly. In the brain, hybridisation signals were also observed in postmitotic but not yet terminally differentiated cells. Therefore, gene expression of p53 does not appear to be linked with cellular proliferation in this organ. A proposed role for p53 in cellular differentiation is discussed.

scholarly journals Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells.

1982 ◽  
Vol 94 (2) ◽  
pp. 253-262 ◽  
Author(s):  
E M Jones-Villeneuve ◽  
M W McBurney ◽  
K A Rogers ◽  
V I Kalnins
Keyword(s):  
Retinoic Acid ◽  
Glial Cells ◽  
Embryonal Carcinoma ◽  
Acetylcholinesterase Activity ◽  
Cell Types ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Neurofilament Protein ◽  
Acid Toxicity ◽  
Precocious Development

Murine embryonal carcinoma cells can differentiate into a varied spectrum of cell types. We observed the abundant and precocious development of neuronlike cells when embryonal carcinoma cells of various pluripotent lines were aggregated and cultured in the presence of nontoxic concentrations of retinoic acid. Neuronlike cells were also formed in retinoic acid-treated cultures of the embryonal carcinoma line, P19, which does not differentiate into neurons in the absence of the drug. The neuronal nature of these cells was confirmed by their staining with antiserum directed against neurofilament protein in indirect immunofluorescence experiments. Retinoic acid-treated cultures also contained elevated acetylcholinesterase activity. Glial cells, identified by immunofluorescence analysis of their intermediate filaments, and a population of fibroblastlike cells were also present in retinoic acid-treated cultures of P19 cells. We did not observe embryonal carcinoma, muscle, or epithelial cells in these cultures. Neurons and glial cells appeared in cultures exposed to retinoic acid for as little as 48 h. We found no evidence for retinoic acid toxicity, suggesting that the effect of the drug was to induce the development of neurons and glia rather than to select against cells differentiating along other developmental pathways.

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