scholarly journals Modulation of the transport of a lysosomal enzyme by PDGF.

1990 ◽  
Vol 110 (2) ◽  
pp. 319-326 ◽  
Author(s):  
E M Prence ◽  
J M Dong ◽  
G G Sahagian
Keyword(s):  
Secretory Pathway ◽  
Cathepsin L ◽  
Control Level ◽  
3T3 Cells ◽  
Lysosomal Protease ◽  
Affinity Ligand ◽  
The Media ◽  
Mouse Cells ◽  
Nih 3T3 ◽  
The Relationship

The major excreted protein (MEP) of transformed mouse fibroblasts is the lysosomal protease, cathepsin L. MEP is also secreted by untransformed mouse cells in response to growth factors and tumor promoters, and is thought to play a role in cell growth and transformation. To determine the relationship between MEP synthesis and MEP secretion, we have examined these events in PDGF-treated NIH 3T3 cells. PDGF enhanced MEP synthesis and caused the diversion of MEP from the lysosomal delivery pathway to a secretory pathway. These two effects were found to be regulated independently at various times after growth factor addition. Short PDGF treatments (0.5 or 1 h) resulted in quantitative secretion of MEP although synthesis was near the control level. High levels of both synthesis and secretion occurred between 2 and 14 h of PDGF treatment. Between 18 and 30 h, the amount of secreted MEP returned to the low control level even though synthesis remained elevated. The secretion was specific for MEP; other lysosomal enzymes were not found in the media from PDGF-treated cells. PDGF-induced secretion of MEP was inhibited 84% by cycloheximide, suggesting that protein synthesis is required to elicit this effect. PDGF also caused a time-dependent increase in mannose 6-phosphate (Man-6-P) receptor-mediated endocytosis. These data support a model in which PDGF alters the distribution of Man-6-P receptors such that the Golgi concentration of receptors becomes limiting, thereby causing the selective secretion of the low affinity ligand, MEP.

scholarly journals Use of a cloned multidrug resistance gene for coamplification and overproduction of major excreted protein, a transformation-regulated secreted acid protease.

1988 ◽  
Vol 8 (8) ◽  
pp. 3316-3321 ◽  
Author(s):  
S E Kane ◽  
B R Troen ◽  
S Gal ◽  
K Ueda ◽  
I Pastan ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cathepsin L ◽  
Protein A ◽  
Acid Protease ◽  
3T3 Cells ◽  
Multidrug Resistance Gene ◽  
Sarcoma Virus ◽  
3T3 Cell Lines ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

Malignantly transformed mouse fibroblasts synthesize and secrete large amounts of major excreted protein (MEP), a 39,000-dalton precursor to an acid protease (cathepsin L). To evaluate the possible role of this protease in the transformed phenotype, we transfected cloned genes for mouse or human MEP into mouse NIH 3T3 cells with an expression vector for the dominant, selectable human multidrug resistance (MDR1) gene. The cotransfected MEP sequences were efficiently coamplified and transcribed during stepwise selection for multidrug resistance in colchicine. The transfected NIH 3T3 cell lines containing amplified MEP sequences synthesized as much MEP as did Kirsten sarcoma virus-transformed NIH 3T3 cells. The MEP synthesized by cells transfected with the cloned mouse and human MEP genes was also secreted. Elevated synthesis and secretion of MEP by NIH 3T3 cells did not change the nontransformed phenotype of these cells.

Use of a cloned multidrug resistance gene for coamplification and overproduction of major excreted protein, a transformation-regulated secreted acid protease

1988 ◽  
Vol 8 (8) ◽  
pp. 3316-3321
Author(s):  
S E Kane ◽  
B R Troen ◽  
S Gal ◽  
K Ueda ◽  
I Pastan ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cathepsin L ◽  
Protein A ◽  
Acid Protease ◽  
3T3 Cells ◽  
Multidrug Resistance Gene ◽  
Sarcoma Virus ◽  
3T3 Cell Lines ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

Malignantly transformed mouse fibroblasts synthesize and secrete large amounts of major excreted protein (MEP), a 39,000-dalton precursor to an acid protease (cathepsin L). To evaluate the possible role of this protease in the transformed phenotype, we transfected cloned genes for mouse or human MEP into mouse NIH 3T3 cells with an expression vector for the dominant, selectable human multidrug resistance (MDR1) gene. The cotransfected MEP sequences were efficiently coamplified and transcribed during stepwise selection for multidrug resistance in colchicine. The transfected NIH 3T3 cell lines containing amplified MEP sequences synthesized as much MEP as did Kirsten sarcoma virus-transformed NIH 3T3 cells. The MEP synthesized by cells transfected with the cloned mouse and human MEP genes was also secreted. Elevated synthesis and secretion of MEP by NIH 3T3 cells did not change the nontransformed phenotype of these cells.

Diversification propaganda work with foreign audiences

2020 ◽  
Vol 4 (4(13)) ◽  
pp. 31-50
Author(s):  
Shiyu Zhang ◽  
Keyword(s):  
Developing Countries ◽  
International Relations ◽  
Strategic Partnership ◽  
The Past ◽  
Bilateral Relations ◽  
The Media ◽  
The Relationship

Over the past decade, bilateral relations between China and Russia have attracted the attention of the whole world. As neighbors and rapidly developing countries, China and Russia are becoming increasingly important in the international arena. The strategic partnership and interaction between China and Russia occupy a significant place in the politics of both countries. Cooperation is developing dynamically in various fields, primarily in politics. After 2012, a change of government took place in China and Russia, which brought new changes to international relations. Studying the involvement of the media in this process can clarify their impact on international relations, in particular, their role in the relationship between China and Russia.

Mediatizing Sex

2019 ◽  
Author(s):  
Crispin Thurlow
Keyword(s):  
New Media ◽  
Academic Writing ◽  
Lived Experiences ◽  
Digital Technologies ◽  
Communicative Practices ◽  
Digital Discourse ◽  
The Media ◽  
The Relationship

This chapter focuses on sex/uality in the context of so-called new media and, specifically, digital discourse: technologically mediated linguistic or communicative practices, and mediatized representations of these practices. To help think through the relationship among sex, discourse, and (new) media, the discussion focuses on sexting and two instances of sexting “scandals” in the news. Against this backdrop, the chapter sets out four persistent binaries that typically shape public and academic writing about sex/uality and especially digital sex/uality: new-old, mediation-mediatization, private/real-public/fake, and personal-political. These either-or approaches are problematic, because they no longer account for the practical realities and lived experiences of both sex and media. Scholars interested in digital sex/uality are advised to adopt a “both-and” approach in which media (i.e., digital technologies and The Media) both create pleasurable, potentially liberating opportunities to use our bodies (sexually or otherwise) and simultaneously thwart us, shame us, or shut us down. In this sense, there is nothing that is really “new” after all.

Metabolism of n-6 fatty acids by NIH-3T3 cells transfected with the ras oncogene

1994 ◽  
Vol 139 (1) ◽  
pp. 71-81 ◽  
Author(s):  
R. J. de Antueno ◽  
R. C. Cantrill ◽  
Y-S. Huang ◽  
G. W. Ells ◽  
M. Elliot ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ras Oncogene ◽  
3T3 Cells ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

scholarly journals Sex Dolls in the Swedish Media Discourse: Intimacy, Sexuality, and Technology

2021 ◽  
Author(s):  
Robin Björkas ◽  
Mariah Larsson
Keyword(s):  
Critical Discourse Analysis ◽  
Critical Discourse ◽  
Sexual Practices ◽  
Media Discourse ◽  
Positive Effects ◽  
Therapeutic Uses ◽  
Broad Variety ◽  
The Media ◽  
Definition Of ◽  
The Relationship

AbstractSex dolls are a complex phenomenon with several diverse possible emotional, sexual and therapeutic uses. They can be part of a broad variety of sexual practices, and also function as a sexual aid. However, the media discourse on sex dolls first and foremost concerns how we perceive the relationship between intimacy and technology. A critical discourse analysis of the Swedish media discourse on sex dolls reveals six themes which dominate the discourse: (a) the definition of what a human being is; (b) a discourse on the (technological and existential) future; (c) a social effort; (d) a loveless phenomenon; (e) men’s violence against women; and (f) pedophilia. Accordingly, this discourse is very conservative and normative in its view of sexuality, technology, and humanity. Overall, the dominant themes do not provide any space for positive effects of technology on human sexuality, and if they do, it is usually as a substitute for something else.

More or More of the Same: Ownership Concentration and Media Diversity in Egypt

2021 ◽  
pp. 194016122110251
Author(s):  
Zahraa Badr
Keyword(s):  
Public Sphere ◽  
State Ownership ◽  
The State ◽  
Ownership Concentration ◽  
Private Ownership ◽  
Media Diversity ◽  
Content Diversity ◽  
The Media ◽  
The Relationship

The Egyptian media has witnessed various changes in the ownership spectrum after the 2011 revolution. To explore this evolution, and through the Habermasian lens, this study examined ownership concentration in the 2019 media sphere in Egypt by mapping media outlets and their owners. It also investigated the relationship between this concentration and content diversity in a sample of print outlets in the first quarter of 2019. Three patterns of ownership concentration in the Egyptian media were identified: concentrated state ownership, concentrated private ownership, and not concentrated private ownership. Based on these findings, I argue that the media sphere in Egypt is dominated by a few gatekeepers, mostly the state, that influence content diversity and jeopardize the democratic public sphere in postrevolution Egypt.

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