scholarly journals THE ULTRASTRUCTURE AND HISTOPHYSIOLOGY OF HUMAN ECCRINE SWEAT GLANDS

1961 ◽  
Vol 11 (2) ◽  
pp. 385-402 ◽  
Author(s):  
Bryce L. Munger
Keyword(s):  
Electron Microscopy ◽  
Sweat Glands ◽  
Basal Cells ◽  
Clear Cells ◽  
Before And After ◽  
Cellular Components ◽  
Mucoid Cells ◽  
Eccrine Sweat Glands ◽  
Pilocarpine Iontophoresis ◽  
Eccrine Sweat

The electron microscopy of human eccrine sweat glands has been studied before and after stimulation by pilocarpine iontophoresis. The identity of the dark and clear cells in the secretory segment as defined by Montagna et al. (23) was determined by studying serial sections, thin for electron microscopy and thick for light microscopy. Cells with numerous apical secretory vacuoles are termed mucoid (dark) cells, since these vacuoles stain positively for acid mucopolysaccharide. Clear cells are intimately associated with intercellular canaliculi. The "cuticular border" of surface cells of the duct is a condensation of tonofilaments and granules. Numerous mitochondria are concentrated in basal cells of the duct. The presence of mucoid cells in the secretory segment may bear on the interpretation of the pathologic findings in the disease cystic fibrosis of the pancreas, and suggests that this disease may be due to a basic disorder of mucopolysaccharide production. The possible roles of the various cellular components in the elaboration of sweat are discussed.

Freeze-Fracture Examination of Tight Junctions of Human Eccrine Sweat Glands

1980 ◽  
Vol 38 ◽  
pp. 634-635
Author(s):  
J. V. Briggman ◽  
J. Bigelow ◽  
H. Bank ◽  
S. S. Spicer
Keyword(s):  
Cystic Fibrosis ◽  
Freeze Fracture ◽  
Sweat Glands ◽  
Hypertonic Solution ◽  
Dark Cells ◽  
Clear Cells ◽  
Junctional Complexes ◽  
Secretory Coil ◽  
Eccrine Sweat Glands ◽  
Eccrine Sweat

The prevalence of strands shown by freeze-fracture in the zonula occludens of junctional complexes is thought to correspond closely with the transepi-thelial electrical resistance and with the tightness of the junction and its obstruction to paracellular flow.1 The complexity of the network of junc¬tional complex strands does not appear invariably related to the degree of tightness of the junction, however, as rabbit ileal junctions have a complex network of strands and are permeable to lanthanum. In human eccrine sweat glands the extent of paracellular relative to transcellular flow remains unknown, both for secretion of the isotonic precursor fluid by the coil and for resorption of a hypertonic solution by the duct. The studies reported here undertook, therefore, to determine with the freeze-fracture technique the complexity of the network of ridges in the junctional complexes between cells in the secretory coil and the sweat ducts. Glands from a patient with cystic fibrosis were also examined because an alteration in junctional strands could underlie the decreased Na+ resorption by sweat ducts in this disease. Freeze-fracture replicas were prepared by standard procedures on isolated coil and duct segments of human sweat glands. Junctional complexes between clear cells, between dark cells and between clear and dark cells on the main lumen, and between clear cells on intercellular canaliculi of the coil con¬tained abundant anastomosing closely spaced strands averaging 6.4 + 0.7 (mean + SE) and 9.0 +0.5 (Fig. 1) per complex, respectively. Thus, the junctions in the intercellular canaliculi of the coil appeared comparable in complexity to those of tight epithlia. Occasional junctions exhibited, in addition, 2 to 5 widely spaced anastomosing strands in a very close network basal to the compact network. The fewer junctional complexes observed thus far between the superficial duct cells consisted on the average of 6 strands arranged in a close network and 1 to 4 underlying strands that lay widely separated from one another (Fig. 2). The duct epitelium would, thus, be judged slightly more "leaky" than the coil. Infrequent junctional complexes observed to date in the secretory coil segment of a cystic fibrosis specimen disclosed rela¬tively few closely crowded strands.

scholarly journals Electron Microscopy of the Mouse Plantar Eccrine Sweat Glands

1970 ◽  
Vol 31 (3-4) ◽  
pp. 455-475 ◽  
Author(s):  
Kazumasa KUROSUMI ◽  
Utako KUROSUMI
Keyword(s):  
Electron Microscopy ◽  
Sweat Glands ◽  
Eccrine Sweat Glands ◽  
Eccrine Sweat

scholarly journals AN ELECTRON MICROSCOPIC STUDY OF ECCRINE SWEAT GLANDS OF THE CAT FOOT AND TOE PADS—EVIDENCE FOR DUCTAL REABSORPTION IN THE HUMAN

1961 ◽  
Vol 11 (2) ◽  
pp. 403-417 ◽  
Author(s):  
Bryce L. Munger ◽  
Saul W. Brusilow
Keyword(s):  
Light And Electron Microscopy ◽  
Sweat Glands ◽  
Striking Difference ◽  
Electron Microscopic ◽  
Ductal Cells ◽  
Before And After ◽  
The Difference ◽  
Foot Pad ◽  
Eccrine Sweat Glands ◽  
Eccrine Sweat

The eccrine sweat glands of the cat foot and toe pads have been studied by light and electron microscopy before and after stimulation with mecholyl. The ultrastructure of these glands in the cat is found to be entirely comparable to that in the human (13). The ultrastructure and staining properties of the secretory segment of the two species are identical. The ductal part of the feline gland is shorter and the ductal cells have only scant mitochondria as compared with the human. Since Brusilow et al. (1) have observed that the secretion of the cat foot pad is isotonic as compared with human sweat, which is hypotonic, and since the secretory segments of the two species are structurally identical, the striking difference in the morphology of the duct is regarded as being responsible for the difference in the chemistry of the secretion of the two species. Thus the duct in the human is capable of reabsorbing sodium and chloride.

scholarly journals HISTOCHEMICAL DIFFERENTIATION OF TWO FORMS OF GLYCOGEN SYNTHETASE

1970 ◽  
Vol 18 (10) ◽  
pp. 756-759 ◽  
Author(s):  
ALLEN A. SMITH
Keyword(s):  
Rhesus Monkeys ◽  
The Other ◽  
Sweat Glands ◽  
Synthetase Activity ◽  
Clear Cells ◽  
Glycogen Synthetase ◽  
Eccrine Sweat Glands ◽  
Active Sweat Glands ◽  
Eccrine Sweat

Glycogen synthetase exists in two forms; one form is dependent on glucose 6-phosphate while the other is not. Variations in the levels of activities of the two forms of the enzyme were observed histochemically in active and inactive eccrine sweat glands from eight rhesus monkeys. The clear cells of active sweat glands showed less glucose 6-phosphate-independent glycogen synthetase activity than the clear cells of inactive sweat glands.

scholarly journals Inflammatory Skin Lesions in Three SARS-CoV-2 Swab-Negative Adolescents: A Possible COVID-19 Sneaky Manifestation?

2021 ◽  
Vol 13 (2) ◽  
pp. 181-188
Author(s):  
Giuseppe Ingravallo ◽  
Francesco Mazzotta ◽  
Leonardo Resta ◽  
Sara Sablone ◽  
Gerardo Cazzato ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Respiratory Symptoms ◽  
Clinical Manifestations ◽  
Skin Lesions ◽  
Sweat Glands ◽  
Histological Findings ◽  
Immune Mediated ◽  
Nodular Lesions ◽  
Immunohistochemical Evidence ◽  
Eccrine Sweat Glands

Coronavirus disease 19 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is associated with various clinical manifestations, including skin lesions. In particular, during the COVID-19 pandemic lock-down period numerous chilblain-like lesions, mainly located on the feet, were observed in adolescents. The latter were often asymptomatic or associated with very mild respiratory symptoms. Here, we report three cases of acral nodular lesions in SARS-CoV-2 swab-negative adolescents with histological findings of chronic immune-mediated inflammation and immunohistochemical evidence of SARS-CoV-2 spike glycoproteins in endothelial cells and eccrine sweat glands. In one of these cases, the virus presence was confirmed by electron microscopy.

scholarly journals Training of Eccrine Sweat Glands in Paraplegics

1970 ◽  
Vol 54 (3) ◽  
pp. 229-232 ◽  
Author(s):  
Becky B. Johnson ◽  
Robert E. Johnson
Keyword(s):  
Sweat Glands ◽  
Eccrine Sweat Glands ◽  
Eccrine Sweat
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