scholarly journals Human interleukin 1 beta is not secreted from hamster fibroblasts when expressed constitutively from a transfected cDNA.

1988 ◽  
Vol 107 (2) ◽  
pp. 447-456 ◽  
Author(s):  
P R Young ◽  
D J Hazuda ◽  
P L Simon
Keyword(s):  
Interleukin 1 ◽  
Surface Membrane ◽  
Chinese Hamster ◽  
Fibroblast Cell ◽  
Interleukin 1 Beta ◽  
Trypsin Treatment ◽  
Transfected Cells ◽  
Chinese Hamster Fibroblast ◽  
Low Levels ◽  
Neomycin Resistance

To understand the secretion and processing of interleukin-1 (IL-1), a Chinese hamster fibroblast cell line (R1610) was transfected with a human IL-1 beta cDNA under the control of the SV40 early promoter and linked to the gene for neomycin resistance. After selecting for transfected cells resistant to G418, two clones were found to constitutively express the IL-1 beta 31-kD precursor which was almost exclusively located in the cytosol. Pulse-chase experiments failed to show any secretion of IL-1 and very little IL-1 activity was detectable in cell supernatants. Furthermore, surface membrane IL-1 activity could not be detected, although low levels of activity could be released upon brief trypsin treatment. Therefore, unlike monocytes, these fibroblast cells lack the mechanism for secreting and processing of IL-1 beta.

Final Report on the Safety Assessment of Polyvinyl Acetate

1992 ◽  
Vol 11 (4) ◽  
pp. 465-473 ◽  
Keyword(s):  
Safety Assessment ◽  
Polyvinyl Acetate ◽  
Chinese Hamster ◽  
Safety Evaluation ◽  
Fibroblast Cell ◽  
Final Report ◽  
Cosmetic Products ◽  
Ames Assay ◽  
Negative Results ◽  
Chinese Hamster Fibroblast

Polyvinyl Acetate, as used in cosmetic products, is a latex emulsion known as the homopolymer of ethenyl acetate. It is used as a binder, emulsion stabilizer, and hair fixative at concentrations less than 25%. Polyvinyl Acetate was nonmutagenic in the Ames assay, with and without activation, and in the Chinese Hamster fibroblast cell assay. Carcinogenic implantation studies using mice gave negative results. It is concluded that the data available are insufficient to support the safety of Polyvinyl Acetate as currently used in cosmetic products. The types of data needed to complete the safety evaluation are listed in the report.

scholarly journals A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells.

1995 ◽  
Vol 14 (9) ◽  
pp. 1914-1922 ◽  
Author(s):  
C. Faucheu ◽  
A. Diu ◽  
A.W. Chan ◽  
A.M. Blanchet ◽  
C. Miossec ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Converting Enzyme ◽  
Transfected Cells

Oxygen toxicity in control and H2O2-resistant Chinese hamster fibroblast cell lines

1990 ◽  
Vol 279 (2) ◽  
pp. 249-260 ◽  
Author(s):  
Douglas R. Spitz ◽  
James H. Elwell ◽  
Yi Sun ◽  
Larry W. Oberley ◽  
Terry D. Oberley ◽  
...  
Keyword(s):  
Cell Lines ◽  
Chinese Hamster ◽  
Oxygen Toxicity ◽  
Fibroblast Cell ◽  
Chinese Hamster Fibroblast ◽  
Fibroblast Cell Lines

High-Level Secretory Expression and Purification of Recombinant Human Interleukin 1 Beta in Pichia pastoris

2016 ◽  
Vol 23 (8) ◽  
pp. 763-769 ◽  
Author(s):  
Pengfei Li ◽  
Ganggang Yang ◽  
Xiaofang Geng ◽  
Jinbao Shi ◽  
Bin Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Expression And Purification ◽  
Secretory Expression ◽  
High Level
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