scholarly journals Multiple influences of a heparin-binding growth factor on neuronal development.

1987 ◽  
Vol 104 (3) ◽  
pp. 635-643 ◽  
Author(s):  
D Schubert ◽  
N Ling ◽  
A Baird
Keyword(s):  
Growth Factor ◽  
Nerve Cell ◽  
Neuronal Development ◽  
Ganglion Cells ◽  
Biological Effects ◽  
Primary Cultures ◽  
Ciliary Ganglion ◽  
Heparin Binding ◽  
Sympathetic Nerve Cell ◽  
Cellular Aggregates

Heparin-binding growth factor-2 (HBGF-2; also known as basic fibroblast growth factor) is mitogenic for most anchorage-dependent cells. It is shown here that HBGF-2 stimulates cell-substratum adhesion and neurite extension in the sympathetic nerve cell line PC12. When HBGF-2 is adsorbed to artificial extracellular matrices consisting of heparin or chondroitin sulfate, it causes the formation of cellular aggregates or circles of cells, respectively. HBGF-2 is also a nerve cell survival molecule, for it potentiates the survival of primary cultures of embryonic chick ciliary ganglion cells but not of embryonic neural retina cells. Finally, a series of synthetic peptides from the HBGF-2 sequence is described that selectively alter the biological effects of HBGF-2. The amphiphilic nature of one of these peptides is discussed with respect to its ability to stimulate cell adhesion.

Analysis of function and expression of the chick GPA receptor (GPAR alpha) suggests multiple roles in neuronal development

Development ◽  
1995 ◽  
Vol 121 (8) ◽  
pp. 2681-2693 ◽  
Author(s):  
S. Heller ◽  
T.P. Finn ◽  
J. Huber ◽  
R. Nishi ◽  
M. Geissen ◽  
...  
Keyword(s):  
Neuronal Development ◽  
Ganglion Cells ◽  
Biological Effects ◽  
Sympathetic Neurons ◽  
Amacrine Cells ◽  
Multiple Roles ◽  
Retinal Ganglion ◽  
Neuronal Populations ◽  
Growth Promoting ◽  
Analysis Of Function

Growth promoting activity (GPA) is a chick growth factor with low homology to mammalian ciliary neurotrophic factor (CNTF) (47% sequence identity with rat CNTF) but displays similar biological effects on neuronal development. We have isolated a chick cDNA coding for GPA receptor (GPAR alpha), a GPI-anchored protein that is 70% identical to hCNTFR alpha. Functional analysis revealed that GPAR alpha mediates several biological effects of both GPA and CNTF. Soluble GPAR alpha supports GPA- and CNTF-dependent survival of human TF-1 cells. In sympathetic neurons, GPAR alpha mediates effects of both GPA and CNTF on the expression of vasoactive intestinal peptide (VIP) as shown by the inhibition of GPA- and CNTF-mediated VIP induction upon GPAR alpha antisense RNA expression. These results demonstrate that GPAR alpha is able to mediate effects of two neurokines that are only distantly related. GPAR alpha mRNA expression is largely restricted to the nervous system and was detected in all neurons that have been shown to respond to GPA or CNTF by increased survival or differentiation, i.e. ciliary, sympathetic, sensory dorsal root, motoneurons, retinal ganglion cells and amacrine cells. Interestingly, GPAR alpha mRNA was additionally found in neuronal populations and at developmental periods not known to be influenced by GPA or CNTF, suggesting novel functions for GPAR alpha and its ligands during neurogenesis and neuron differentiation.

scholarly journals Mechanism for diminished tissue factor expression by endothelial cells cultured with heparin binding growth factor-1 and heparin

Blood ◽  
1991 ◽  
Vol 77 (6) ◽  
pp. 1256-1262 ◽  
Author(s):  
FE Almus ◽  
LV Rao ◽  
UR Pendurthi ◽  
L Quattrochi ◽  
SI Rapaport
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Tissue Factor ◽  
Umbilical Vein ◽  
Blot Hybridization ◽  
Primary Cultures ◽  
Cdna Probe ◽  
Enzyme Linked Immunosorbent Assay ◽  
Northern Blot Hybridization ◽  
Heparin Binding

Abstract We have extended our earlier observation that growing primary cultures of human umbilical vein endothelial cells (HUVEC) with heparin binding growth factor 1 (HBGF-1) 20 micrograms/mL and heparin 12 U/mL inhibits expression of tissue factor (TF) activity on HUVC monolayers perturbed with thrombin. TF activity was measured as the ability of monolayers or cell lysates to support FVIIa-catalyzed activation peptide release from 3H-FX. TF antigen in HUVEC extracts was measured in an enzyme-linked immunosorbent assay (ELISA) that uses a double-antibody sandwich technique with rabbit and goat antibodies to human TF. TF-mRNA was measured by Northern blot hybridization with a 32P-TF cDNA probe. Cells growth with HBGF-1/heparin had both decreased surface and total TF activity as compared with HUVEC from the same endothelial cell pool grown without HBGF-1/heparin. Means +/- SD for TF antigen for four primary cultures were 4.4 +/- 0.9 ng/10(6) cells without HBGF-1/heparin and 0.6 +/- 0.3 ng/10(6) cells with HBGF-1/heparin. TF mRNA 4 hours after incubation with thrombin of HUVEC grown without HBGF-1/heparin was about sevenfold higher than TF mRNA of HUVEC grown with HBGF- 1/heparin. These data establish that growing primary cultures of HUVEC with HBGF-1/heparin impairs their ability to synthesize TF apoprotein after perturbation. This may be part of a generalized response of endothelial cells to HBGF-1/heparin facilitating migration during angiogenesis.

Platelet-derived growth factor (PDGF) receptors in the developing mouse optic pathway

1994 ◽  
Vol 11 (1) ◽  
pp. 33-40 ◽  
Author(s):  
James B. Hutchins ◽  
Xiaorong Zhang
Keyword(s):  
Nervous System ◽  
Growth Factor ◽  
Glial Cells ◽  
Neuronal Development ◽  
Ganglion Cells ◽  
Platelet Derived Growth Factor ◽  
Intercellular Signaling ◽  
The Central Nervous System ◽  
Process Outgrowth

AbstractThe molecules which control the patterns of cell division, growth, and precise interconnections characteristic of the central nervous system still remain largely unidentified. The protein platelet-derived growth factor (PDGF) has been shown to mediate interactions among glial cells in vitro. More recent evidence has indicated that PDGF may also be involved in controlling communication between neurons and glial cells and among neurons. The presence of receptors for PDGF on neurons of the developing nervous system is an essential piece of evidence in this chain of events. Ganglion cells are labeled with antibodies to PDGF receptor only during the period of active process outgrowth. These findings suggest that PDGF is used as a mediator of intercellular signaling during neuronal development.

scholarly journals Mechanism for diminished tissue factor expression by endothelial cells cultured with heparin binding growth factor-1 and heparin

Blood ◽  
1991 ◽  
Vol 77 (6) ◽  
pp. 1256-1262 ◽  
Author(s):  
FE Almus ◽  
LV Rao ◽  
UR Pendurthi ◽  
L Quattrochi ◽  
SI Rapaport
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Tissue Factor ◽  
Umbilical Vein ◽  
Blot Hybridization ◽  
Primary Cultures ◽  
Cdna Probe ◽  
Enzyme Linked Immunosorbent Assay ◽  
Northern Blot Hybridization ◽  
Heparin Binding

We have extended our earlier observation that growing primary cultures of human umbilical vein endothelial cells (HUVEC) with heparin binding growth factor 1 (HBGF-1) 20 micrograms/mL and heparin 12 U/mL inhibits expression of tissue factor (TF) activity on HUVC monolayers perturbed with thrombin. TF activity was measured as the ability of monolayers or cell lysates to support FVIIa-catalyzed activation peptide release from 3H-FX. TF antigen in HUVEC extracts was measured in an enzyme-linked immunosorbent assay (ELISA) that uses a double-antibody sandwich technique with rabbit and goat antibodies to human TF. TF-mRNA was measured by Northern blot hybridization with a 32P-TF cDNA probe. Cells growth with HBGF-1/heparin had both decreased surface and total TF activity as compared with HUVEC from the same endothelial cell pool grown without HBGF-1/heparin. Means +/- SD for TF antigen for four primary cultures were 4.4 +/- 0.9 ng/10(6) cells without HBGF-1/heparin and 0.6 +/- 0.3 ng/10(6) cells with HBGF-1/heparin. TF mRNA 4 hours after incubation with thrombin of HUVEC grown without HBGF-1/heparin was about sevenfold higher than TF mRNA of HUVEC grown with HBGF- 1/heparin. These data establish that growing primary cultures of HUVEC with HBGF-1/heparin impairs their ability to synthesize TF apoprotein after perturbation. This may be part of a generalized response of endothelial cells to HBGF-1/heparin facilitating migration during angiogenesis.

Hepatoma-Derived Growth Factor (HDGF): A New Heparin-Binding Growth Factor Identified in Leiomyomas and Normal Myometrium

1998 ◽  
Vol 5 (1) ◽  
pp. 180A-180A
Author(s):  
M ISHIKAWA ◽  
S TAKASHIMA ◽  
H NAKAMURA ◽  
M KLAGSBRUN ◽  
R NOWAK
Keyword(s):  
Growth Factor ◽  
Heparin Binding
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