scholarly journals Antibodies against a lysosomal membrane antigen recognize a prelysosomal compartment involved in the endocytic pathway in cultured prolactin cells.

1985 ◽  
Vol 100 (3) ◽  
pp. 786-793 ◽  
Author(s):  
C Tougard ◽  
D Louvard ◽  
R Picart ◽  
A Tixier-Vidal
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Strong Acid ◽  
Cell Types ◽  
Lysosomal Membrane ◽  
Endocytic Pathway ◽  
Gh3 Cells ◽  
Prolactin Cells ◽  
Coated Pits

Antibodies against a lysosomal membrane antigen (A-Ly-M) have recently been obtained and characterized (Reggio, H., D. Bainton, E. Harms, E. Coudrier, and D. Louvard, 1984, J. Cell Biol., 99:1511-1526). They recognize a 100,000-mol-wt antigen immunologically related to a purified [H+,K+]ATPase from pig gastric mucosa. In the present study, we have localized this antigen during adsorptive endocytosis in rat prolactin cells in culture using cationized ferritin (CF) as a tracer. CF was rapidly internalized (after 5 min) in coated pits and vesicles that were labeled by antibodies against clathrin. The tracer was then delivered (after 15 min) to vacuoles and multivesicular bodies. These structures were labeled with A-Ly-M. These organelles were devoid of acid phosphatase activity. At later stages (after 30 min) CF was observed within larger structures that were strongly stained by A-Ly-M and displayed a strong acid phosphatase activity. These findings clearly indicate that A-Ly-M react with prelysosomal and lysosomal compartments involved in the endocytic pathway in cultured prolactin cells. The membrane of these structures therefore contains antigenic determinant(s) related to the 100,000-mol-wt polypeptide. Our results suggest that the prelysosomal structure stained by A-Ly-M may represent in GH3 cells the acidic prelysosomal compartment recently described in the early steps of endocytosis in other cell types (Tycko, B., and F. R. Maxfield, 1982, Cell, 28:643-651).

The host-parasite interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea)

Parasitology ◽  
1968 ◽  
Vol 58 (2) ◽  
pp. 371-375 ◽  
Author(s):  
David A. Erasmus
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Plasma Membranes ◽  
Science Research ◽  
Strong Acid ◽  
Gland Cells ◽  
Adhesive Organ ◽  
Cyathocotyle Bushiensis

A combination of histochemical and electron microscope techniques have demonstrated, in Cyathocotyle bushiensis, alkaline phosphatase activity in the matrix of the tegument, in the distal and basal plasma membranes of the tegument, in the wall of the ducts extending from the adhesive organ gland cells and in the wall of the adhesive organ microvilli. Acid phosphatase activity was much stronger and was present in the tegument matrix and in the granular component of the secretion from the adhesive organ gland cells. Strong acid phosphatase activity was also present in the cisternae of the endoplasmic reticulum of the adhesive organ gland cells.I am greatly indebted to Professor Brough for the excellent facilities available within this department. I also wish to thank Professor J. Sinclair (Department of Mining) for electron microscope facilities extended to me in the early stages of this investigation, and to Mr W. Henderson, Mr T. Davies and Miss M. Williams for their invaluable assistance. The purchase of the Huxley ultramicrotome, coating unit and an AEI EM 6 electron microscope was made possible by a grant from the Science Research Council.

ULTRASTRUCTURAL LOCALIZATION OF A CHARACTERISTIC ACID PHOSPHATASE IN GRANULES OF RABBIT BASOPHILS

1974 ◽  
Vol 22 (12) ◽  
pp. 1092-1104 ◽  
Author(s):  
ATSUSHI KOMIYAMA ◽  
SAMUEL S. SPICER
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Potential Role ◽  
Strong Acid ◽  
Ultrastructural Localization ◽  
Buffy Coat ◽  
Cytoplasmic Granules ◽  
Nitrophenyl Phosphate ◽  
Tris Maleate

Bone marrow basophils incubated in Gomori medium at pH 6.0-6.8 exhibited strong acid phosphatase activity suggestive of a potential role in endocytosis in one-third of the cytoplasmic granules and also in Golgi elements. Buffy coat basophils contained about one-third as many reactive granules. Reaction product was confined to the threadlike component of the larger granules predominant in early basophils and was absent from the denser-type granules predominant in late basophils. In centrioles of basophils acid phosphatase appeared localized between triplet fibers. Reactivity with the Gomori medium was diminished at pH 5.0, absent at pH 8.0 and only slightly decreased with p-nitrophenyl phosphate as substrate. Basophils incubated in Barka-Anderson medium at pH 5.0-6.8 revealed light acid phosphatase activity in the Golgi lamellae but essentially none in cytoplasmic granules. Tris-maleate buffer of the Barka-Anderson medium replacing the sodium acetate of the Gomori medium inhibited the reactivity in the granules. Incubation in media containing NaF, or lacking substrate, eliminated the heavy precipitates in granules and Golgi elements but yielded light, nonenzymatic lead staining in Golgi and tubulovesicular structures and atypical granules present only in buffy coat basophils.

Myeloblasts of Acute Granulocytic Leukemia

1973 ◽  
Vol 31 ◽  
pp. 390-391
Author(s):  
G. June Marshall ◽  
Mary E. Kirchen ◽  
Anne D. Geddes ◽  
Jos. R. Bateman
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Cell Types ◽  
Mature Cell ◽  
Maturation Arrest ◽  
Standard Methods ◽  
Cacodylate Buffer ◽  
Granulocytic Leukemia

Acute granulocytic leukemia (AGL) is typified by a maturation arrest resulting from a synthetic block which is reflected in morphologically identifiable stages of maturation. In AGL's the marrow population primarily consists of two cell types: the myeloblast which is the least mature cell and one by definition which lacks lysosomes and the promyelocyte which contains lysosomes. Maturation beyond the promyelocyte is an aberrant process which results in a limited number of abnormal mature granulocytes. In order to better characterize the cells, marrows from 11 individuals with AGL have been studied at the LM and EM levels. All marrows were obtained prior to the initiation of chemotherapy. Fixation was with 5% gluteraldehyde in 0.1M cacodylate buffer. Osmication, dehydration and embedding were accomplished by standard methods. All marrows were reacted for peroxidase and acid phosphatase activity.

scholarly journals Enzyme histochemical studies on the pathological changes in human Sertoli cells.

1982 ◽  
Vol 30 (12) ◽  
pp. 1268-1274 ◽  
Author(s):  
H G Goslar ◽  
B Hilscher ◽  
S G Haider ◽  
N Hofmann ◽  
D Passia ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Acid Phosphatase Activity ◽  
Cell Damage ◽  
Strong Acid ◽  
Testicular Biopsy ◽  
Biopsy Material

Two forms of human Sertoli cell disorders were characterized enzyme histochemically from the testicular biopsy material of infertile and subfertile patients. Sertoli cell asthenia: a slight injury of the Sertoli cell with exfoliation of individual germ cells; marked by the rarefaction of reaction zones of thiamine pyrophosphatase (TPPase) and a decrease in lactate dehydrogenase (LDH). Sertoli cell insufficiency: severe Sertoli cell damage with the formation of a "puff" and a heavy exfoliation of germ cells (dislocation of Sertoli cell nucleus and cytoplasm along with the related germ cells into the lumen of seminiferous tubule); marked by a heterogeneous activity pattern of TPPase, the disappearance of LDH, maintenance of a slightly weakened activity of alkaline phosphatase, and an increase of acid phosphatase. In the case of Sertoli-cell-only syndrome, the high prismatic Sertoli cells showed strong acid phosphatase activity with scattered weak TPPase reaction, whereas the flat or cube-like Sertoli cells exhibited weak acid phosphatase activity with only one small round reaction zone of TPPase in each cell. In addition, the frequency of the occurrence of Sertoli cell asthenia, Sertoli cell insufficiency, and Sertoli-cell-only syndrome is reported, and its correlation with the andrological diseases discussed.

Erythrophagocytosis in the Liver in Hemolytic Anemias

1968 ◽  
Vol 26 ◽  
pp. 184-185
Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent
Keyword(s):  
Acid Phosphatase ◽  
Oxidative Damage ◽  
Phosphatase Activity ◽  
Kupffer Cells ◽  
Acid Phosphatase Activity ◽  
Common Type ◽  
Red Cell ◽  
Cell Fragments ◽  
Reticulo Endothelial System ◽  
Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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