scholarly journals THE STRUCTURE OF THE SARCOLEMMA OF THE FROG SKELETAL MUSCLE FIBER

1961 ◽  
Vol 10 (4) ◽  
pp. 177-185 ◽  
Author(s):  
Alexander Mauro ◽  
W. Robert Adams
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Skeletal Muscle Fiber ◽  
Frog Skeletal Muscle

Nalorphine: actions at an opiate receptor on frog skeletal muscle fibers

1983 ◽  
Vol 61 (11) ◽  
pp. 1361-1367
Author(s):  
Thomas E. Ary ◽  
George B. Frank
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Fiber ◽  
Partial Agonist ◽  
Opiate Receptor ◽  
Skeletal Muscle Fiber ◽  
Frog Skeletal Muscle ◽  
Full Agonist ◽  
Opiate Antagonists ◽  
Sodium And Potassium

Intracellular microelectrode studies were conducted to investigate the actions of the partial agonist – antagonist nalorphine at an opiate receptor on functional frog skeletal muscle fiber membranes. In high bath concentrations (≥ 10−4 M), nalorphine alone produces agonist actions similar to the "full" opiate agonists. These actions were (i) to depress both the sodium and potassium (gNa and gK) conductance increases due to electrical stimulation by a nonspecific local anestheticlike mechanism and (ii) to depress gNa by a specific opiate receptor mediated mechanism. In a much lower bath concentration (1 × 10−8 M) nalorphine acts to antagonize the specific opiate receptor mediated depression of gNa produced by the "full" agonist meperidine. Thus in this preparation nalorphine, "the partial antagonist," has the same actions as naloxone, which is often considered to be a full antagonist. The quantitative differences observed in the effects of these two opiate antagonists are discussed.

Time-resolved synchrotron X-ray diffraction studies of a single frog skeletal muscle fiber

1991 ◽  
Vol 39 (3) ◽  
pp. 287-297 ◽  
Author(s):  
Masato Konishi ◽  
Katsuzo Wakabayashi ◽  
Satoshi Kurihara ◽  
Hideo Higuchi ◽  
Noboru Onodera ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Skeletal Muscle Fiber ◽  
Frog Skeletal Muscle ◽  
X Ray Diffraction ◽  
Time Resolved ◽  
X Ray

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

1992 ◽  
Vol 50 (1) ◽  
pp. 776-777
Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Action Potential ◽  
Muscle Fiber ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Electrical Field Stimulation ◽  
Skeletal Muscle Fiber ◽  
Freeze Dried ◽  
Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

Sign in / Sign up

Export Citation Format

Share Document