HYPOTHYROIDISM AND NUTRITIONAL STATUS ALTER 3,5,3′-TRIIODOTHYRONINE (T3) RECEPTORS IN ISOLATED LIVER NUCLEI OFANABAS TESTUDINEUS(BLOCH)

2001 ◽  
Vol 27 (3) ◽  
pp. 329-336
Author(s):  
B. Shameena ◽  
T. R. Renuka ◽  
S. Varghese ◽  
C. S. Paulose ◽  
O. V. Oommen
Author(s):  
William E. Lynch ◽  
Tetsuhiko Umeda ◽  
Masaru Uyeda ◽  
Irving Lieberman

1980 ◽  
Vol 28 (6) ◽  
pp. 552-556 ◽  
Author(s):  
D Cocchia ◽  
F Michetti

Antibodies against the loosely bound subnuclear protein fraction (0.35 M NaCl-extractable subnuclear fraction) of rat brain were raised in rabbits, and the ultrastructural distribution of the antigenic determinants in rat cerebellum was studied using the unlabeled antibody peroxidase-antiperoxidase (PAP) method. A localization restricted to the nucleus was observed. Both neuronal and neuroglial nuclei exhibited antigens, whereas nuclei of pericytes and endothelial cells did not. The immunoreaction product was homogeneously distributed in dispersed chromatin and was absent from condensed chromatin, suggesting that the antigens were confined to the active regions of the genoma. The outer nuclear membrane and the nucleolus appeared to be free of the antigens, while a perinucleolar ring of immunoreaction was detectable. Liver preparations showed a nuclear reaction markedly weaker than the one for brain nuclei. Adsorption of the serum with isolated liver nuclei nullified the reactivity in liver tissue, whereas a sharp reaction was still observed in the cerebellum, indicating the subcellular reaction under examination to contain antigens specifically concentrated in the nervous system or unique to the brain.


1956 ◽  
Vol 2 (2) ◽  
pp. 127-145 ◽  
Author(s):  
K. J. Monty ◽  
M. Litt ◽  
E. R. M. Kay ◽  
A. L. Dounce

1. The significance of the term nucleolus has been discussed. 2. A detailed method for the isolation of nucleoli from already isolated rat or cat liver nuclei has been presented. 3. The presence of DNA in isolated liver cell nucleoli has been indicated by histochemical methods. 4. The percentages of DNA and RNA in the isolated nucleoli have been determined by chemical analysis. 5. The specific activities of aldolase, arginase, and catalase have been determined for two subnuclear fractions and for the isolated nucleoli of rat and cat liver, and the relative amounts of these enzymes in the same subnuclear fractions and nucleoli of rat liver have been measured. 6. The significance of the above findings has been discussed and consideration has been given to what types of isolated nuclei might best serve as starting material for the isolation of nucleoli. 7. A new hypothesis has been presented that nucleoli of the liver cell type may function primarily in furnishing (directly or indirectly) templates for the synthesis of the particular enzymes that must govern the chemistry of mitosis.


FEBS Letters ◽  
1987 ◽  
Vol 215 (2) ◽  
pp. 252-256 ◽  
Author(s):  
Brenda L. Haug ◽  
John T. Sibley ◽  
Jeremy S. Lee
Keyword(s):  

Biochemistry ◽  
1979 ◽  
Vol 18 (17) ◽  
pp. 3674-3679 ◽  
Author(s):  
Jo Alene Golidl

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