Synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) copolymer produced in sequential feeding fed batch cultures of ralstonia eutropha

2002 ◽  
Vol 51 (7) ◽  
pp. 607-617 ◽  
Author(s):  
Gracida Rodríguez Jorge Noel ◽  
Alba Flores Joel ◽  
Pérez-Guevara Fermín ◽  
Cardoso Martínez Judith
Keyword(s):  
Ralstonia Eutropha ◽  
Synthesis And Characterization ◽  
Fed Batch ◽  
Batch Cultures

Production and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) synthesized by Ralstonia eutropha in fed-batch cultures

Polymer ◽  
2000 ◽  
Vol 41 (10) ◽  
pp. 3499-3505 ◽  
Author(s):  
L.A Madden ◽  
A.J Anderson ◽  
J Asrar ◽  
P Berger ◽  
P Garrett
Keyword(s):  
Ralstonia Eutropha ◽  
Fed Batch ◽  
Batch Cultures

scholarly journals Metabolic characterization of a CHO cell size increase phase in fed-batch cultures

2017 ◽  
Vol 101 (22) ◽  
pp. 8101-8113 ◽  
Author(s):  
Xiao Pan ◽  
Ciska Dalm ◽  
René H. Wijffels ◽  
Dirk E. Martens
Keyword(s):  
Cell Size ◽  
Size Increase ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures

scholarly journals Fed-batch production and characterization of polyhydroxybutyrate by Bacillus megaterium LVN01 from residual glycerol

DYNA ◽  
2020 ◽  
Vol 87 (214) ◽  
pp. 111-120
Author(s):  
Javier Ricardo Gómez Cardozo ◽  
Rodrigo Velasco Bucheli ◽  
Nathalia Marín Pareja ◽  
Orlando Simón Ruíz Villadiego ◽  
Guillermo Antonio Correa Londoño ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Operating Conditions ◽  
Fed Batch ◽  
Fermentation Time ◽  
Batch Cultures ◽  
Batch Systems ◽  
Dry Cell Weight ◽  
Phb Production ◽  
Dry Cell

The operating conditions of polyhydroxybutyrate (PHB) production processes are among the factors that most influence yields. In this study, we evaluated PHB production synthesized by Bacillus megaterium LVN01. Batch and fed-batch cultures were used to produce PHB from residual glycerol. For this, dry cell weight (DCW) and PHB productivity were analyzed at a preliminary stage by central composite design using batch systems under different temperature, C/N ratio, and fermentation time conditions. The maximum PHB productivity occurred at 30.8 °C, 44.9 mol mol-1, and 39.9 h. The same conditions were tested for studies in fed-batch culture. Fed-batch experiments were comparable to each other, where the DCW was around 1.9 g L-1, with PHB productivities of 29.5 mg L-1 h-1 and 35.6 mg L-1 h-1 for bioreactors of 5 L and 14 L, respectively. The PHB was characterized by NMR, FTIR, DSC, TGA, and DTG analys

scholarly journals Biosynthesis and characterization of biodegradable Poly(3-hydroxybutyrate) from renewable sources

2008 ◽  
Vol 13 (1) ◽  
pp. 1-11 ◽  
Author(s):  
S.M.F. Pereira ◽  
R.S. Rodriguez ◽  
J.G.C. Gomes
Keyword(s):  
Soybean Oil ◽  
Ralstonia Eutropha ◽  
Chemical Shifts ◽  
Carbon Sources ◽  
Gel Permeation Chromatography ◽  
Crystallinity Degree ◽  
Batch Cultures ◽  
H Nmr ◽  
Biodegradable Poly

Poly(3-hydroxybutyrate) was produced in fed-batch cultures of Ralstonia eutropha DSM 428 and Alcaligenes latus ATCC 29712 on a mineral medium with different carbon sources such as sucrose, sodium lactate, lactic acid, soybean oil and fatty acid. The bacteria converted the different carbon sources supplied into P3HB. The best results were obtained when lactate or soybean oil were supplied as the sole carbon source. The range of number average molar mass (Mn) for the polymers, analyzed by Gel Permeation Chromatography was 1.65 to 0.79 x 10(5) g mol-1. FTIR spectroscopy revealed a characteristic absorbance associated with polyester structures. The crystallinity degree, determinate from X-ray diffractograms, was about 69% in all synthesized polymers. The thermal properties associated to semicrystalline polymers indicated a glass transition at 0.1ºC and a melting point at about 175ºC and enthalpy of 63- 89 J g-1. The ¹H-NMR and 13C-NMR spectra of the polymers were in agreement with the calculated chemical shifts associated with P3HB structures.

Synthesis and Characterization of the Three Dicyclopentapyrenes

1996 ◽  
Vol 61 (10) ◽  
pp. 3572-3572
Author(s):  
Lawrence T. Scott ◽  
Atena Necula
Keyword(s):  
Synthesis And Characterization
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