Structural characterization of products from fuel-rich combustion: An approach based on size exclusion chromatography

2002 ◽  
Vol 174 (11-12) ◽  
pp. 345-359 ◽  
Author(s):  
B. Apicella ◽  
A. Ciajolo ◽  
I. Suelves ◽  
T. J. Morgan ◽  
A. A. Herod ◽  
...  
Keyword(s):  
Size Exclusion Chromatography ◽  
Structural Characterization ◽  
Size Exclusion ◽  
Exclusion Chromatography

scholarly journals New G-Quadruplex-Forming Oligodeoxynucleotides Incorporating a Bifunctional Double-Ended Linker (DEL): Effects of DEL Size and ODNs Orientation on the Topology, Stability, and Molecularity of DEL-G-Quadruplexes

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 654 ◽  
Author(s):  
Maria Marzano ◽  
Andrea Falanga ◽  
Stefano D’Errico ◽  
Brunella Pinto ◽  
Giovanni Roviello ◽  
...  
Keyword(s):  
Size Exclusion Chromatography ◽  
Structural Characterization ◽  
Size Exclusion ◽  
Biological Processes ◽  
Dna Nanostructures ◽  
Nmr Studies ◽  
Molecular Weights ◽  
G Quadruplex ◽  
Exclusion Chromatography

G-quadruplexes (G4s) are unusual secondary structures of DNA occurring in guanosine-rich oligodeoxynucleotide (ODN) strands that are extensively studied for their relevance to the biological processes in which they are involved. In this study, we report the synthesis of a new kind of G4-forming molecule named double-ended-linker ODN (DEL-ODN), in which two TG4T strands are attached to the two ends of symmetric, non-nucleotide linkers. Four DEL-ODNs differing for the incorporation of either a short or long linker and the directionality of the TG4T strands were synthesized, and their ability to form G4 structures and/or multimeric species was investigated by PAGE, HPLC–size-exclusion chromatography (HPLC–SEC), circular dichroism (CD), and NMR studies in comparison with the previously reported monomeric tetra-ended-linker (TEL) analogues and with the corresponding tetramolecular species (TG4T)4. The structural characterization of DEL-ODNs confirmed the formation of stable, bimolecular DEL-G4s for all DEL-ODNs, as well as of additional DEL-G4 multimers with higher molecular weights, thus suggesting a way towards the obtainment of thermally stable DNA nanostructures based on reticulated DEL-G4s.

Sign in / Sign up

Export Citation Format

Share Document