Bioremediation of Crude Oil-Contaminated Soil Using Slurry-Phase Biological Treatment and Land Farming Techniques

2003 ◽  
Vol 12 (1) ◽  
pp. 85-99 ◽  
Author(s):  
Maria S. Kuyukina ◽  
Irena B. Ivshina ◽  
Marina I. Ritchkova ◽  
James C. Philp ◽  
Colin J. Cunningham ◽  
...  
2021 ◽  
Vol 1107 (1) ◽  
pp. 012167
Author(s):  
R. Babalola ◽  
V.E. Efeovbokhan ◽  
Y. O. Atiku ◽  
U.E. Usoro ◽  
M. A. Ibeh ◽  
...  

Author(s):  
N. Christofi ◽  
I. B. Ivshina ◽  
M. S. Kuyukina ◽  
J. C. Philp

2017 ◽  
Vol 6 (2) ◽  
pp. 168-174
Author(s):  
Abubakar Tuhuloula ◽  
Ali Altway ◽  
Sri Rachmania Juliastuti ◽  
Suprapto Suprapto

Pollution by chrysene compounds in the polluted soil of petroleum, due to exploration activities, production and disposal of petroleum waste into the environment causing serious damage to the ecosystem environment, became the target of processing with bacteria as a model of remediation of pollution sites. Thus, the study focused on the use of a bacterial consortium to degrade chrysene in petroleum-contaminated soil. The study was conducted by mixing 20:80 (% wt) of contaminated soil with water. The consortium of Bacillus cereus and Pseudomonas putida 10%(v/v) and 15%(v/v) bacteria with ratios; 2:3; 1:1; 3:2 is inserted into the slurry bioreactor. Biodegradation process is run with agitation of 100 rpm and temperature 26<sup>o</sup>C 30<sup>o</sup>C and in aeration. Identification of chrysene using gas chromatographymass spectrometry (GCMS) and bacterial populations with haemycitometer. The initial concentration of chrysene is 24.48 ng/?L. After 49 days remediation period for a 10% (v/v) reduced chrysene bacteria consortium and bacterial populations were 8.68 ng/?L; 7.56 ng/?L; and 8.07 ng/?L; with biodegradation rate is 67.01%; 69.10%; And 64.54%. As for the 15% (v/v) bacteria consortium with the same ratio, chrysene was degraded to 2.60 ng/?L; 1.57 ng/?L; and 2.02 ng/?L and the measured chrysene biodegradation rate was 89.39%; 93.58%; And 91.73%. These findings suggest that the percentage of low crude oil is degraded because of the increasing concentration of crude oil.


1997 ◽  
Vol 1997 (1) ◽  
pp. 932-935 ◽  
Author(s):  
Manuel Correa ◽  
Elizabeth Padrón ◽  
Irene Petkoff

ABSTRACT In November 1994, a 16-inch pipeline rupture resulted in a spill of about 10,000 barrels of crude oil extending on 4 hectares of agricultural land, including part of a freshwater lagoon. The spill was contained 2 kilometers away from the accident by a dike constructed across the lagoon during the first response operation. Oil cleanup activities included a bioremediation process (land farming) where harrowing, water irrigation, and fertilization of contaminated soil were applied Physical removal qfthe oil with sorbents, booms, and pads, aswell as a drum skimmer, were used to dean up and restore the lagoon. Laboratory tests reported mat total crude oübiodegrodation rates were stabilized within 2 months, with a mean total hydrocarbons degradation of 80%.


2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Ehizonomhen S. Okonofua ◽  
Kayode H. Lasisi ◽  
Eguakhide Atikpo

AbstractLand farming technique was used to treat hydrocarbon contaminated soil collected from a crude oil spill sites in Edo State, Nigeria. Calibrated standard auger was used to collect soil samples from the site at depth below 30 cm. The samples were characterized and classified. Cow dung and NPK fertilizer were added as additives to complement the nutriments of the soil samples before total petroleum hydrocarbon (TPH) quantification and remediation procedures. Factorial design was applied to vary the input parameters such as pH, mass of substrate, moisture content and turning times of land farming so to ascertain the optimal conditions for the procedure. The result revealed that the in-situ TPH value was 5000 mg kg− 1 on the average and after 90 d of treatment, TPH reduced to 646 mg kg− 1. The turning rate, pH, moisture content and mass of substrate hade 83, 4.36, 0.48 and 0.046% contribution, respectively, for the degradation process using land farming treatment. Numerical optimization techniques applied in the optimum point for land farming input parameters to achieve predicted maximum removal of 99% were evaluated as pH, mass of substrate, moisture content and turning rate to be 6.01, 1 kg, 10% and 5 times in a week, respectively. TPH removed at this optimum point was 98% reducing from 5000 to 636 mg kg− 1. The high coefficient of determination (r2 = 0.9865) as observed in the closeness of predicted and experimental values reflects the reliability of the model and hence, land farming practice with close attention on turning rate as revealed by this study, is recommended for TPH contaminated soil remediation.


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