Lipid-independent effects of an estrogen-statin combination: inhibition of expression of adhesion molecules and plasminogen activator inhibitor-1 in human endothelial cell cultures

Climacteric ◽  
2001 ◽  
Vol 4 (3) ◽  
pp. 209-214 ◽  
Author(s):  
H. Seeger ◽  
D. Wallwiener ◽  
A. O. Mueck
Keyword(s):  
Endothelial Cell ◽  
Plasminogen Activator ◽  
Adhesion Molecules ◽  
Cell Cultures ◽  
Plasminogen Activator Inhibitor ◽  
Human Endothelial Cell ◽  
Plasminogen Activator Inhibitor 1 ◽  
Endothelial Cell Cultures ◽  
Independent Effects ◽  
Activator Inhibitor

scholarly journals Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5'-end lipophilic modification.

1999 ◽  
Vol 46 (3) ◽  
pp. 679-691 ◽  
Author(s):  
A Kobylańska ◽  
E Pluskota ◽  
M Swiatkowska ◽  
M Wójcik ◽  
A Cierniewska-Cieślak ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Plasminogen Activator ◽  
Cell Cultures ◽  
Antisense Oligonucleotides ◽  
Plasminogen Activator Inhibitor ◽  
Mechanism Of Inhibition ◽  
Endothelial Cell Cultures ◽  
Human Plasminogen ◽  
Activator Inhibitor ◽  
Pai 1

A series of conjugates containing residues of lipophilic alcohols covalently bound to 5' end of oligodeoxyribonucleotides targeted against human plasminogen activator inhibitor (PAI-1) mRNA was synthesized via the oxathiaphospholane approach. The highest anti-PAI-1 activity in EA.hy 926 endothelial cell cultures was found for conjugates containing menthyl or heptadecanyl groups linked with an oligonucleotide complementary to a segment of human PAI-1 mRNA. The phosphodiester antisense oligonucleotides, which otherwise exhibit only limited anti-PAI-1 activity, were found to be more active than phosphorothioate oligonucleotides when conjugated to lipophilic alcohol residues. For menthyl conjugates an evidence of antisense mechanism of inhibition was found.

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