scholarly journals Characterization of aggregates produced by the potential mycoherbistatPlectosporium alismatisin submerged culture: germination, UV-radiation tolerance and infectivity

2011 ◽  
Vol 21 (10) ◽  
pp. 1243-1256 ◽  
Author(s):  
Sophie Cliquet ◽  
Julien Despreaux ◽  
Kashif Zeeshan ◽  
Denis de la Broise ◽  
Gavin Ash
Keyword(s):  
Uv Radiation ◽  
Submerged Culture ◽  
Radiation Tolerance

Production and characterization of biomass and exopolysaccharides obtained in submerged culture under different initial pHs used in the cultivation of Colletotrichum gloeosporioides and Rhizopus stolonifer

2020 ◽  
pp. 628
Author(s):  
Juan Diego Valenzuela Cobos ◽  
René Oscar Rodríguez-Grimón ◽  
Ana Grijalva-Endara ◽  
Raúl Marcillo-Vallejo ◽  
Onay Adonys Mercader-Camejo
Keyword(s):  
Yeast Extract ◽  
Submerged Culture ◽  
Liquid Culture ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Mycelial Biomass ◽  
Rhizopus Stolonifer

Colletotrichum gloeosporioides (GC003) and Rhizopus stolonifer (RS001) were cultivated in two different liquid culture media: LC1 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 2 g L-1) and LC2 (glucose 40 g L-1, yeast extract 3 g L-1 and tryptone peptone 10 g L-1) for the production of mycelial biomass and exopolysaccharides (EPS). By using the liquid culture (LC2) under pH of 4.5 presented the highest biomass content (15.73 g L-1) in the propagation of Rhizopus stolonifer. The highest production of exopolysaccharides (1.74 g L-1) was obtained by the liquid culture (LC2) under pH of 4.5 in the cultivation of Colletotrichum gloeosporioides. The results presented that the production of biomass and exopolysaccharides (EPS) is directly related with the pHs values and the strain used in the cultivation.

scholarly journals A New Approach to UV Protection by Direct Surface Functionalization of TiO2 with the Antioxidant Polyphenol Dihydroxyphenyl Benzimidazole Carboxylic Acid

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 231 ◽  
Author(s):  
Mattia Battistin ◽  
Valeria Dissette ◽  
Alessandro Bonetto ◽  
Elisa Durini ◽  
Stefano Manfredini ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Uv Radiation ◽  
Surface Functionalization ◽  
Uv Filters ◽  
Protection Factor ◽  
Full Characterization ◽  
New Class ◽  
Product Formulation ◽  
Booster Effect

Skin cancer is the most common malignant cancer with an incidence of 1 million cases/year. It is well known that exposure to UV radiation from sunlight leads the most frequent risk factors for several skin disorders including skin cancer. Sunscreen filters represent a valid protection against dangerous effects derived from UV radiation, and they can be divided in organic and inorganic UV filters. Adding, at the product formulation, molecules with booster effect, or also substances that can increase the protecting effectiveness via synergic mechanisms, can further enhance their protection activity. Moreover, this approach leads to develop formulations with high SPF (Sun Protection Factor) with a reduced content of UV filters, this is in line with the recent decisions of yet a few countries (Palau, Thailand, Philippines, and Hawaii) to ban some sunscreen filters to preserve marine environments (i.e., reef). In this work, a new class of sunscreen UV filters has been synthesized, by means the combination of physical filter and Oxisol, an antioxidant molecule with booster effect. In this study, the synthesis of new physical multifunctional ingredients is reported, by means the direct surface functionalization of inorganic filters (in particular TiO2) with Oxisol. In this study, the full characterization of these multifunctional ingredients is also reported, in addition to the cytotoxicity tests, the photocatalytic activity and the rheological properties involved on skin application.

scholarly journals Sequence Diversity of rulA among Natural Isolates of Pseudomonas syringae and Effect on Function of rulAB-Mediated UV Radiation Tolerance

2000 ◽  
Vol 66 (12) ◽  
pp. 5167-5173 ◽  
Author(s):  
George W. Sundin ◽  
Janette L. Jacobs ◽  
Jes�s Murillo
Keyword(s):  
Amino Acid ◽  
Pseudomonas Syringae ◽  
Uv Radiation ◽  
Radiation Sensitivity ◽  
Amino Acid Sequences ◽  
Radiation Tolerance ◽  
Plant Host ◽  
Long Time ◽  
Natural Isolates ◽  
Wavelength Radiation

ABSTRACT The rulAB locus confers tolerance to UV radiation and is borne on plasmids of the pPT23A family in Pseudomonas syringae. We sequenced 14 rulA alleles from P. syringae strains representing seven pathovars and found sequence differences of 1 to 12% within pathovar syringae, and up to 15% differences between pathovars. Since the sequence variation withinrulA was similar to that of P. syringaechromosomal alleles, we hypothesized that rulAB has evolved over a long time period in P. syringae. A phylogenetic analysis of the deduced amino acid sequences of rulAresulted in seven clusters. Strains from the same plant host grouped together in three cases; however, strains from different pathovars grouped together in two cases. In particular, the rulAalleles from P. syringae pv. lachrymans and P. syringae pv. pisi were grouped but were clearly distinct from the other sequenced alleles, suggesting the possibility of a recent interpathovar transfer. We constructed chimeric rulABexpression clones and found that the observed sequence differences resulted in significant differences in UV (wavelength) radiation sensitivity. Our results suggest that specific amino acid changes in RulA could alter UV radiation tolerance and the competitiveness of theP. syringae host in the phyllosphere.

scholarly journals Screening of endophytic sources of exopolysaccharides: Preliminary characterization of crude exopolysaccharide produced by submerged culture of Diaporthe sp. JF766998 under different cultivation time

2016 ◽  
Vol 2 ◽  
pp. 33-40 ◽  
Author(s):  
Ravely Casarotti Orlandelli ◽  
Ana Flora Dalberto Vasconcelos ◽  
João Lúcio Azevedo ◽  
Maria de Lourdes Corradi da Silva ◽  
João Alencar Pamphile
Keyword(s):  
Submerged Culture ◽  
Preliminary Characterization ◽  
Cultivation Time

Phenotypic and genetic characterization of Paecilomyces lilacinus strains with biocontrol activity against root-knot nematodes

2000 ◽  
Vol 46 (9) ◽  
pp. 775-783 ◽  
Author(s):  
T S Gunasekera ◽  
R J Holland ◽  
M R Gillings ◽  
D A Briscoe ◽  
D C Neethling ◽  
...  
Keyword(s):  
Uv Radiation ◽  
Nematophagous Fungus ◽  
Paecilomyces Lilacinus ◽  
Candidate Species ◽  
Genetic Clusters ◽  
Screening Assays ◽  
Phenotypic And Genetic Characterization ◽  
Efficient Selection ◽  
Selection Of

Efficient selection of fungi for biological control of nematodes requires a series of screening assays. Assessment of genetic diversity in the candidate species maximizes the variety of the isolates tested and permits the assignment of a particular genotype with high nematophagous potential using a rapid novel assay. Molecular analyses also facilitate separation between isolates, allowing the identification of proprietary strains and trace biocontrol strains in the environment. The resistance of propagules to UV radiation is an important factor in the survival of a biocontrol agent. We have analyzed 15 strains of the nematophagous fungus Paecilomyces lilacinus using these principles. Arbitrarily primed DNA and allozyme assays were applied to place the isolates into genetic clusters, and demonstrated that some genetically related P. lilacinus strains exhibit widespread geographic distributions. When exposed to UV radiation, some weakly nematophagous strains were generally more susceptible than effective isolates. A microtitre tray-based assay used to screen the pathogenic activity of each isolate to Meloidogyne javanica egg masses revealed that the nematophagous ability varied between 37%-100%. However, there was no clear relationship between nematophagous ability and genetic clusters. Molecular characterizations revealed sufficient diversity to allow tracking of strains released into the environment.Key words: Paecilomyces lilacinus, LP-RAPD analysis, allozymes, UV sensitivity.

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