Dose – response for radiation-induced apoptosis, residual 53BP1 foci and DNA-loop relaxation in human lymphocytes

2005 ◽  
Vol 81 (2) ◽  
pp. 125-138 ◽  
Author(s):  
J Torudd ◽  
M Protopopova ◽  
R Sarimov ◽  
J Nygren ◽  
S Eriksson ◽  
...  
Keyword(s):  
Dose Response ◽  
Human Lymphocytes ◽  
Dna Loop ◽  
Radiation Induced ◽  
Induced Apoptosis

Modification of radiation-induced apoptosis in radiation- or hyperthermia-adapted human lymphocytes

1994 ◽  
Vol 72 (11-12) ◽  
pp. 475-482 ◽  
Author(s):  
S. P. Cregan ◽  
D. R. Boreham ◽  
P. R. Walker ◽  
D. L. Brown ◽  
R. E. J. Mitchel
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Adaptive Response ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Fluorescence Analysis ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Protective Mechanism ◽  
Radiation Induced ◽  
Induced Apoptosis

We have investigated the influence of the cellular adaptive response to ionizing radiation on radiation-induced apoptosis in human cells. The adaptive response is believed to be a protective mechanism that confers resistance to the detrimental effects of ionizing radiation and that can be induced by different agents, including hyperthermia and radiation. We have used fluorescence analysis of DNA unwinding (FADU) to assay the induction of apoptosis in human peripheral blood lymphocytes by ionizing radiation. Using the FADU assay, we have observed the initial radiation-induced DNA damage, its subsequent disappearance due to enzymatic repair, and its time- and dose-dependent reappearance. We believe this reappearance of DNA damage to be indicative of the DNA fragmentation event associated with apoptosis. This interpretation has been supported at the individual cell level using an in situ terminal deoxynucleotidyl transferase (TDT) assay (Apoptag, Oncor Inc.), which detects the 3′-hydroxyl ends of fragmented DNA, and by fluorescence analysis of nuclear morphology in Hoechst 33258 stained cells. Pretreatment of cells with low-dose γ-radiation (0.1 Gy) or mild hyperthermia (40 °C for 30 min) altered the extent of radiation-induced (3 Gy) apoptosis. Both pretreatments sensitized lymphocytes to become apoptotic after the 3-Gy radiation exposure. This sensitization may represent an adaptive response mechanism that reduces the risk that genetically damaged cells will proliferate. The ability to modify the probability of radiation-induced apoptosis may lower the cancer risk from a radiation exposure.Key words: apoptosis, adaptive response, ionizing radiation, hyperthermia.

Radiation-Induced Apoptosis in Human Lymphocytes and Lymphoma Cells Critically Relies on the Up-Regulation of CD95/Fas/APO-1 Ligand

1998 ◽  
Vol 149 (6) ◽  
pp. 588 ◽  
Author(s):  
C. Belka ◽  
P. Marini ◽  
W. Budach ◽  
K. Schulze-Osthoff ◽  
F. Lang ◽  
...  
Keyword(s):  
Human Lymphocytes ◽  
Lymphoma Cells ◽  
Radiation Induced ◽  
Induced Apoptosis

An equation for the dose response of radiation-induced apoptosis

1995 ◽  
Vol 35 (2) ◽  
pp. 165-166 ◽  
Author(s):  
C.Clifton Ling ◽  
C.H. Chen ◽  
Zvi Fuks
Keyword(s):  
Dose Response ◽  
Radiation Induced ◽  
Induced Apoptosis

Radiation-Induced Apoptosis in Human Lymphocytes

1996 ◽  
Vol 71 (5) ◽  
pp. 685-691 ◽  
Author(s):  
D. R. Boreham ◽  
K. L. Gale ◽  
S. R. Maves ◽  
J-A. Walker ◽  
D. P. Morrison
Keyword(s):  
Human Lymphocytes ◽  
Radiation Induced ◽  
Induced Apoptosis

Sci-AM Fri - 04: Radiation induced apoptosis and chromosomal aberrations in human lymphocytes: Estimating the risks associated with radiation exposures

2005 ◽  
Vol 32 (7Part2) ◽  
pp. 2417-2418
Author(s):  
L Ryan ◽  
J Lavoie ◽  
K Schnarr ◽  
M Almardini ◽  
A Ho ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Human Lymphocytes ◽  
Radiation Induced ◽  
Induced Apoptosis
Sign in / Sign up

Export Citation Format

Share Document