Arterial air embolism after venous air infusion in newborn piglets

2001 ◽  
Vol 90 (7) ◽  
pp. 786-792
Author(s):  
E Juvik, E Solligård, A-O Bru
Keyword(s):  
2007 ◽  
Vol 90 (7) ◽  
pp. 786-792 ◽  
Author(s):  
E Juvik ◽  
E Solligård ◽  
A-O Brubakk ◽  
A-M Brubakk
Keyword(s):  

Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.


2015 ◽  
Vol 53 (05) ◽  
Author(s):  
J Theisz ◽  
I Igaz ◽  
L Tóth ◽  
L Topa
Keyword(s):  

2008 ◽  
Vol 22 (4) ◽  
pp. 725-728 ◽  
Author(s):  
Takaharu Yagi ◽  
Kazuhiro Ueda ◽  
Toshiki Tanaka ◽  
Mitsutaka Jinbo ◽  
Kimikazu Hamano

1991 ◽  
Author(s):  
Joseph A. Bettencourt ◽  
Charles M. Harrison ◽  
Theodore Plemons ◽  
Patricia L. Schleiff ◽  
William J. Mehm

2019 ◽  
Vol 20 (7) ◽  
pp. 713-726 ◽  
Author(s):  
Shiyu Tao ◽  
Tianjiao Zhou ◽  
Perot Saelao ◽  
Ying Wang ◽  
Yuhua Zhu ◽  
...  

Intrauterine growth restriction (IUGR) remains a major problem in swine production since the associated low birth weight leads to high rates of pre-weaning morbidity and mortality, and permanent retardation of growth and development. The underlying regulatory mechanisms from the aspects of epigenetic modification has received widespread attention. Studies explore the changes in genome wide methylation in small intestine (SI), liver and longissimus dorsi muscle (LDM) between IUGR and normal birth weight (NBW) newborn piglets using a methylated DNA immunoprecipitation-sequencing (MeDIP-Seq) approach. The data demonstrated that methylated peaks were prominently distributed in distal intergenic regions and the quantities of peaks in IUGR piglets were more than that of NBW piglets. IUGR piglets had relatively high methylated level in promoters, introns and coding exons in all the three tissues. Through KEGG pathway analysis of differentially methylated genes found that 33, 54 and 5 differentially methylated genes in small intestine, liver and longissimus dorsi muscle between NBW and IUGR piglets, respectively, which are related to development and differentiation, carbohydrate and energy metabolism, lipid metabolism, protein turnover, immune response, detoxification, oxidative stress and apoptosis pathway. The objective of this review is to assess the impact of differentially methylation status on developmental delay, metabolic disorders and immune deficiency of IUGR piglets.


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