Molecular pathogenesis of precursor lesions of pancreatic ductal adenocarcinoma

Pathology ◽  
2003 ◽  
Vol 35 (1) ◽  
pp. 14-24 ◽  
Author(s):  
Andrew V. Biankin ◽  
James G. Kench ◽  
Floriaan P. Dijkman ◽  
Sandra A. Biankin ◽  
Susan M. Henshall
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Molecular Pathogenesis ◽  
Ductal Adenocarcinoma ◽  
Precursor Lesions

Molecular pathogenesis of precursor lesions of pancreatic ductal adenocarcinoma

Pathology ◽  
2003 ◽  
Vol 35 (1) ◽  
pp. 14-24 ◽  
Author(s):  
Andrew V. Biankin ◽  
James G. Kench ◽  
Floriaan P. Dijkman ◽  
Sandra A. Biankin ◽  
Susan M. Henshall
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Molecular Pathogenesis ◽  
Ductal Adenocarcinoma ◽  
Precursor Lesions

Molecular characterization of early precursor lesions of pancreatic ductal adenocarcinoma

Pancreatology ◽  
2018 ◽  
Vol 18 (4) ◽  
pp. S4
Author(s):  
Lisa Frohn ◽  
Lena Häberle ◽  
Wolfgang Göring ◽  
Matthias Wirth ◽  
Irene Esposito
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Molecular Characterization ◽  
Ductal Adenocarcinoma ◽  
Precursor Lesions

scholarly journals MicroRNA-21 Is Induced Early in Pancreatic Ductal Adenocarcinoma Precursor Lesions

2010 ◽  
Vol 56 (4) ◽  
pp. 603-612 ◽  
Author(s):  
Maël Chalret du Rieu ◽  
Jérôme Torrisani ◽  
Janick Selves ◽  
Talal Al Saati ◽  
Anny Souque ◽  
...  
Keyword(s):  
Early Detection ◽  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Early Stage ◽  
Survival Rates ◽  
Ductal Adenocarcinoma ◽  
Early Event ◽  
Precursor Lesions ◽  
Tissue Samples ◽  
Ductal Cells

Abstract Background: Pancreatic ductal adenocarcinoma (PDAC) has the poorest overall prognosis among gastrointestinal cancers; however, curative resection in early-stage PDAC greatly improves survival rates, indicating the importance of early detection. Because abnormal microRNA production is commonly detected in cancer, we investigated noninvasive precursor pancreatic intraepithelial neoplasia (PanIN) lesions for microRNA production as a potential early biomarker of PDAC. Methods: Pathologists identified and classified ductal lesions. We extracted total RNA from laser-capture microdissected PanIN tissue samples from a conditional KRAS(G12D) mouse model (n = 29) or of human origin (n = 38) (KRAS is v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog). MicroRNA production was quantified by quantitative real-time PCR. Internal controls included 5S and U6 RNAs. Results: Production of microRNAs miR-21, miR-205, and miR-200 paralleled PanIN progression in the KRAS(G12D) mouse model, compared with microRNA production in samples of nonpathologic ducts. miR-21 demonstrated the highest relative concentrations in the precursor lesions. Interestingly, miR-205 and miR-21 up-regulation preceded phenotypic changes in the ducts. The production of microRNAs miR-21, miR-221, miR-222, and let-7a increased with human PanIN grade, with peak production occurring in hyperplastic PanIN-2/3 lesions. In situ hybridization analysis indicated miR-21 production to be concentrated in pathologic ductal cells. miR-21 production was regulated by KRAS(G12D) and epidermal growth factor receptor in PDAC-derived cell lines. Conclusions: Aberrant microRNA production is an early event in the development of PanIN. Our findings indicate that miR-21 warrants further investigation as a marker for early detection of PDAC.

scholarly journals Molecular pathogenesis of pancreatic ductal adenocarcinoma: Impact of passenger strand of pre-miR-148aon gene regulation

2018 ◽  
Vol 109 (6) ◽  
pp. 2013-2026 ◽  
Author(s):  
Tetsuya Idichi ◽  
Naohiko Seki ◽  
Hiroshi Kurahara ◽  
Haruhi Fukuhisa ◽  
Hiroko Toda ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Molecular Pathogenesis ◽  
Ductal Adenocarcinoma ◽  
Passenger Strand

scholarly journals miRNAs as Diagnostic and Prognostic Biomarkers in Pancreatic Ductal Adenocarcinoma and Its Precursor Lesions: A Review

2015 ◽  
Vol 10 ◽  
pp. BMI.S27679 ◽  
Author(s):  
Bárbara Alemar ◽  
Cleandra Gregório ◽  
Patricia Ashton-Prolla
Keyword(s):  
Pancreatic Cancer ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Clinical Management ◽  
Positive Impact ◽  
Invasive Procedure ◽  
Prognostic Biomarkers ◽  
Premalignant Lesions ◽  
Ductal Adenocarcinoma ◽  
Precursor Lesions ◽  
Comprehensive Review

Pancreatic ductal adenocarcinoma (PDAC), a rare but lethal tumor, is difficult to diagnose without performing an invasive procedure. miRNAs are known to be deregulated in PDAC patients, and recent studies have shown that they can be used as diagnostic and prognostic of the disease. The detection of miRNAs in samples acquired through minimally or noninvasive procedures, such as serum, plasma, and saliva, can have a positive impact on the clinical management of these patients. This article is a comprehensive review of the major studies that have evaluated the expression of miRNAs as biomarkers in pancreatic cancer and its premalignant lesions.

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