Determination of total phosphorus in soil using simple Kjeldahl digestion

2000 ◽  
Vol 31 (15-16) ◽  
pp. 2665-2670 ◽  
Author(s):  
M.D. Taylor
Keyword(s):  
Total Phosphorus ◽  
Kjeldahl Digestion

Microdetermination of Cholesterol and Phospholipid in Cerebrospinal Fluid and Serum by Silicic Acid Column Chromatography

1962 ◽  
Vol 8 (6) ◽  
pp. 598-605 ◽  
Author(s):  
Yung S Shin ◽  
James C Lee
Keyword(s):  
Cerebrospinal Fluid ◽  
Human Serum ◽  
Total Phosphorus ◽  
Silicic Acid ◽  
Column Chromatography ◽  
Silicic Acid Column ◽  
Lipid Phosphorus ◽  
Silicic Acid Column Chromatography ◽  
Phospholipid Ratio

Abstract A method is presented for the determination of cholesterol and phospholipid, which requires 5 µl. of human serum or 1-2 ml. of cerebrospinal fluids. With this method 5-100 µg. of cholesterol and phospholipid can be separated by a modified silicic acid column after elution of the mixture with 1 ml. of chloroform and 3 ml. of methanol. Recovery for 24.6 µg. of cholesterol and 30.5 µg. of phospholipid was 98.4 and 96.7%, respectively. Standard deviations of ± 1.73 and ± 1.24 have been obtained for the reproducibility of cholesterol and phospholipid determinations after chromatography. The method has been applied for the estimation of the cholesterol/phospholipid ratio and of lipid phosphorus in total phosphorus of human cerebrospinal fluids.

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