Factors Affecting the Fertility Level in Poultry, with Special Reference to Artificial Insemination

1983 ◽  
Vol 39 (2) ◽  
pp. 106-117 ◽  
Author(s):  
P. E. Lake
Keyword(s):  
Special Reference ◽  
Artificial Insemination ◽  
Fertility Level ◽  
Factors Affecting

SOME FACTORS AFFECTING PREGNANCY RATE DURING ARTIFICIAL INSEMINATION IN CATTLE

2019 ◽  
Vol 22 (07) ◽  
pp. 118-122
Author(s):  
Haider R. Abbas ◽  
Hussein A. Khamees ◽  
Jawad K. Taher
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Factors Affecting

Cervical Spondylotic Myelopathy: Surgical Results and Factors Affecting Outcome with Special Reference to Age Differences

Neurosurgery ◽  
2003 ◽  
Vol 52 (1) ◽  
pp. 122-126 ◽  
Author(s):  
Tomosato Yamazaki ◽  
Kiyoyuki Yanaka ◽  
Hiroaki Sato ◽  
Kazuya Uemura ◽  
Atsuro Tsukada ◽  
...  
Keyword(s):  
Special Reference ◽  
Age Differences ◽  
Cervical Spondylotic Myelopathy ◽  
Surgical Results ◽  
Factors Affecting

scholarly journals Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

2020 ◽  
Vol 13 (5) ◽  
pp. 947-955
Author(s):  
Zulfi Nur Amrina Rosyada ◽  
Mokhamad Fakhrul Ulum ◽  
Ligaya I. T. A. Tumbelaka ◽  
Bambang Purwantara
Keyword(s):  
Artificial Insemination ◽  
Sperm Quality ◽  
High Fertility ◽  
Fertility Level ◽  
Sperm Protein ◽  
Sperm Proteins ◽  
Frozen Semen ◽  
Sds Page ◽  
Microscopic Evaluation ◽  
Holstein Bulls

Background and Aim: Holstein cows and heifers are widely bred in Indonesia by artificial insemination (AI) to increase population and milk production. Sperm fertility is modulated by genetic factors, but the analysis of sperm quality is still based on macro- and microscopic characteristics. This study aimed to analyze both sperm quality and proteins of Holstein bulls at different fertility levels. Materials and Methods: The frozen semen samples were collected from the Indonesia National AI Center. They were classified based on the reproductive efficiency data and were grouped into high fertile (HF) and low fertile (LF). Sperm qualities were evaluated by microscopic evaluation. The Holstein sperm proteins were extracted using phenylmethanesulfonyl fluoride as a protease inhibitor and the benzidine detergent extraction method. Discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was conducted to analyze the molecular weights (MWs) of the sperm proteins. The data obtained were analyzed by a t-test using the one-factor bull fertility level, and Spearman's correlation analysis was used to identify the correlation between the sperm microscopic evaluation parameters and protein bands. Results: The sperm motility post-freeze thawing was not significantly different between the HF and LF (p>0.05). The HF level had a higher percentage of viability, intact plasma membrane integrity, and intact acrosomes than the LF (p<0.05). Five protein bands were found in the SDS-PAGE of sperm proteins of Holstein bulls with different concentrations. Sperm proteins with MWs of 17.51 kDa, 14.87 kDa, 33.71 kDa, and 41.97 kDa were abundant in the Holstein bulls with an HF level, while 55 kDa proteins were abundant in the LF level of Holstein bulls. The sperm of Holstein bulls in the HF level contained proteins of about 33.71 kDa that were not detected in the LF. Conclusion: The sperm protein with a molecular weight of 33.71 kDa was predicted to be a specific protein biomarker that influences bull fertility. Sperm fertilization abilities were also determined by the sperm proteins, the morphology of sperm acrosomes, and the quality of plasma membranes. This method can be used to select bulls with high fertility to increase the population of Holstein bulls.

Sign in / Sign up

Export Citation Format

Share Document