scholarly journals Non-destructive evaluation of in vitro-stored plants: A comparison of visual and image analysis

2006 ◽  
Vol 42 (6) ◽  
pp. 562-567 ◽  
Author(s):  
Hailu M. Aynalem ◽  
Timothy L. Righetti ◽  
Barbara M. Reed
Keyword(s):  
Image Analysis ◽  
Non Destructive Evaluation ◽  
Non Destructive

DAPI Method: A Novel Assay to Evaluate the In vitro Impact of Nanomaterials on Mammalian Cells

2006 ◽  
Vol 951 ◽  
Author(s):  
Pavan M. V. Raja ◽  
Jennifer Connolley ◽  
Pulickel M. Ajayan ◽  
Omkaram Nalamasu ◽  
Deanna M. Thompson
Keyword(s):  
Image Analysis ◽  
Mammalian Cells ◽  
Biological Impact ◽  
Simple Estimate ◽  
Aortic Smooth Muscle ◽  
Diphenyltetrazolium Bromide ◽  
Wide Range ◽  
The Impact ◽  
Non Destructive

ABSTRACTThe increasing importance of nanomaterial-related applications has given rise to concerns pertaining to their impact on human health. In vitro mammalian cell-based assays can provide a quick and simple estimate of the possible adverse effects of the nanomaterials. However, recent studies have questioned the efficacy of traditional assays such as the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, in evaluating cell-nanomaterial interactions, implying the need for alternate methods. We applied image analysis to enumerate the DAPI (2-[4-(Aminomethyl) phenyl]-1H-indole-6-carboximidamide, dihydrochloride) – stained cellular nuclei. Image analysis, being non-destructive, capable of automation, and applicable over a wide range of cell seeding densities, offers several advantages compared to older methods like the MTT assay and hemocytometry. Using image analysis, the impact of singlewalled carbon nanotubes (SWNT) on rat aortic smooth muscle cell (SMC) growth kinetics, were examined. Despite the carbon nanomaterial presence, the fluorescent signal from the nuclei was not noticeably impacted over the SWNT range examined (0.00-0.10 mg/ml). We anticipate that this method can also be applied to evaluate the biological impact of other nanomaterials.

scholarly journals 2D size, position and shape definition of defects by B-scan image analysis

2015 ◽  
Author(s):  
Michele Scafidi ◽  
Donatella Cerniglia ◽  
Tommaso Ingrassia
Keyword(s):  
Image Analysis ◽  
Cross Section ◽  
Automatic Inspection ◽  
Structural Components ◽  
Laser Ultrasonic ◽  
Non Destructive Evaluation ◽  
Ultrasonic Techniques ◽  
Definition Of ◽  
B Scan Images ◽  
Non Destructive

The non-destructive evaluation of defects by automatic procedures is of great importance for structural components. Thanks to the developments of the non-contact ultrasonic techniques, the automation of the inspections is gaining a progressively important role. In this work, an automatic inspection technique for the evaluation of defects by the analysis of B-scan images obtained by a laser ultrasonic system is presented. The data are extracted directly from a B-scan map obtained for a panel with internal defects, and are used to build an image of the cross section of the panel. The proposed automatic procedure allows the definition of size, position and shape of defects in panels of known thickness.

scholarly journals Growth of pineapple plantlets during acclimatisation can be monitored through automated image analysis of the canopy

2020 ◽  
Vol 4 (4) ◽  
pp. 223-229
Author(s):  
Guillermo Soto ◽  
Gustavo Lorente ◽  
Jessica Mendoza ◽  
Evelio Dany Báez ◽  
Carlos Manuel Lorenzo ◽  
...  
Keyword(s):  
Image Analysis ◽  
Automated Image Analysis ◽  
Plant Phenotyping ◽  
Tropical Fruit ◽  
Planting Material ◽  
Non Destructive Evaluation ◽  
Plantlet Growth ◽  
Canopy Area ◽  
Mathematical Relationships ◽  
Non Destructive

AbstractPineapple is an economically important tropical fruit crop, but the lack of adequate planting material limits its productivity. A range of micropropagation protocols has been developed over the years to address this shortfall. Still, the final stage of micropropagation, i.e. acclimatisation, remains a challenge as pineapple plantlets grow very slowly. Several studies have been conducted focusing on this phase and attempting to improve plantlet growth and establishment, which requires tools for the non-destructive evaluation of growth during acclimatisation. This report describes the use of semi-automated and automated image analysis to quantify canopy growth of pineapple plantlets, during five months of acclimatisation. The canopy area progressively increased during acclimatisation, particularly after 90 days. Regression analyses were performed to determine the relationships between the automated image analysis and morphological indicators of growth. The mathematical relationships between estimations of the canopy area and the fresh and dry weights of intact plantlets, middle-aged leaves (D leaves) and roots showed determination coefficients (R2) between 0.84 and 0.92. We propose an appropriate tool for the simple, objective and non-destructive evaluation of pineapple plantlets growth, which can be generally applied for plant phenotyping, to reduce costs and develop streamlined pipelines for the assessment of plant growth.

scholarly journals Impedance Spectroscopy for the Non-Destructive Evaluation of In Vitro Epidermal Models

2014 ◽  
Vol 32 (5) ◽  
pp. 1845-1854 ◽  
Author(s):  
F. Groeber ◽  
L. Engelhardt ◽  
S. Egger ◽  
H. Werthmann ◽  
M. Monaghan ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Non Destructive Evaluation ◽  
Non Destructive

scholarly journals Comparative Evaluation of Pellet Cushioning Agents by Various Imaging Techniques and Dissolution Studies

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Konrád Sántha ◽  
Nikolett Kállai-Szabó ◽  
Viktor Fülöp ◽  
Géza Jakab ◽  
Péter Gordon ◽  
...  
Keyword(s):  
Image Analysis ◽  
Imaging Techniques ◽  
Pharmaceutical Research ◽  
Good Alternative ◽  
Pharmaceutical Products ◽  
X Ray ◽  
Coated Particle ◽  
The Individual ◽  
Non Destructive

AbstractMost of the commercially available pharmaceutical products for oral administration route are marketed in the tablet dosage forms. However, compression of multiparticulate systems is a challenge for the pharmaceutical research and industry, especially if the individual unit is a coated particle, as the release of the active ingredient depends on the integrity of the coating. In the present study, polymer-coated pellets tableted with different types of excipients (powder, granules, pellets) then were investigated by various tablet-destructive (microscopic) and tablet non-destructive (microfocus X-ray; microCT) imaging methods. The information obtained from the independent evaluation of the in vitro drug release profiles model is confirmed by the results obtained by image analysis, regardless of whether X-ray or stereomicroscopic images of the coated, tableted pellets were used for image analysis. The results of this study show that the novel easy-to-use, fast, and non-destructive MFX method is a good alternative to the already used microscopic image analysis methods regarding the characterization of particulates, compressed into tablets.

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