In vitro zygotic embryo culture of an endangered forest tree Givotia rottleriformis and factors affecting its germination and seedling growth

2006 ◽  
Vol 42 (5) ◽  
pp. 418-421 ◽  
Author(s):  
M. Rambabu ◽  
M. Upender ◽  
D. Ujjwala ◽  
T. Ugandhar ◽  
M. Praveen ◽  
...  
Keyword(s):  
Seedling Growth ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Forest Tree ◽  
Factors Affecting ◽  
Zygotic Embryo Culture

scholarly journals In vitro zygotic embryo culture of Pinus peuce Gris.: Optimization of culture conditions affecting germination and early seedling growth

2012 ◽  
Vol 64 (2) ◽  
pp. 503-509 ◽  
Author(s):  
Dragana Stojicic ◽  
Dusica Janosevic ◽  
Branka Uzelac ◽  
V. Cokesa ◽  
Snezana Budimir
Keyword(s):  
Seedling Growth ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Zygotic Embryos ◽  
Physical Factors ◽  
Zygotic Embryo Culture ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Pinus Peuce

This study reports a protocol for the germination and early seedling growth of Pinus peuce Gris. using zygotic embryo culture. In order to overcome seed dormancy and optimize organogenesis, the effect of nutritional, plant growth regulatory and physical factors on in vitro germination and growth of isolated mature zygotic embryos of P. peuce were investigated.

scholarly journals Standardization of zygotic embryo culture from Nerium oleander L. and comparative analysis of biosynthesized cardiac glycosides within in vitro and acclimatized plants

2021 ◽  
pp. 204-215
Author(s):  
Renu Nimoriya ◽  
Yatendra Singh ◽  
Sumit Kumar Singh ◽  
Pankaj Singh ◽  
Amar Jeet ◽  
...  
Keyword(s):  
Embryo Culture ◽  
Cardiac Glycoside ◽  
Cardiac Glycosides ◽  
Zygotic Embryo ◽  
Germination Percentage ◽  
Zygotic Embryos ◽  
In Vitro Multiplication ◽  
Zygotic Embryo Culture ◽  
In Vitro Plantlets

The primary result of our experiment revealed that the germination percentage of N. oleander mature seeds is only 30%. From this observation, the concept of protocol standardization for zygotic embryo culture of this plant was originated. Zygotic embryo culture was proved an efficient in vitro multiplication system of N. oleander. The maximum germination percentage (96%) of zygotic embryos was observed on ¼ MS medium with 15 gm/L sucrose, whereas the best growth medium was optimized as ½ B5 with same sucrose concentration. The second part of this study was aimed to find out the cardiac glycoside accumulation pattern in both in vitro and acclimatized plants. For this purpose, one-month-old in vitro plantlets and acclimatized plants were subjected to LC-MS analysis and 09 cardiac glycosides were detected and quantified in both the systems. Most of the cardiac glycosides including odoroside A (32.71 mg/gm DW), odoroside H (4.69 mg/gm DW) and oleandrin (0.52 mg/gm DW) were found to be accumulated at maximum level within in vitro plantlets. CG 840b (1.89 mg/gm DW) is the only cardiac glycoside, which was maximally accumulated in acclimatized plants. From this study, it can be concluded that, zygotic embryo culture is a better choice for in vitro multiplication of N. oleander when compared to matured seeds and in vitro grown plantlets of this species favor cardiac glycosides biosynthesis in comparison to acclimatized plants. Therefore, all future research on the enrichment of cardiac glycosides from this plant may be conducted on zygotic embryos derived in vitro grown plantlets or cultures.

Zygotic embryo culture is an efficient way to optimize in vitro growth in Panax ginseng

2021 ◽  
Vol 167 ◽  
pp. 113497
Author(s):  
Jung-Woo Lee ◽  
Gyung-Ran Do ◽  
Ic-Hyun Jo ◽  
Chi-Eun Hong ◽  
Kyung-Hwan Bang ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
In Vitro Growth ◽  
Zygotic Embryo Culture

scholarly journals SEED DORMANCY BREAKING OF AN ENDANGERED MEDICINAL TREE SPECIES (TAXUS BACCATA L.) USING EMBRYO CULTURE

2021 ◽  
Vol 185 (1) ◽  
pp. 1-11
Author(s):  
Mohamed BEKHOUCHE
Keyword(s):  
Seed Dormancy ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Forest Tree ◽  
Garden Soil ◽  
Taxus Baccata ◽  
Dormancy Breaking ◽  
Medicinal Tree ◽  
Growth Room

Natural regeneration of Taxus baccata L. is constrained due to the depth of seed dormancy requirements (often taking two or more years) and low seed germination. Further, the conventional method of vegetative propagation by cuttings is associated with difficulties in rooting. Hence, for the first time, this study describes an efficient and reproducible in vitro protocol for breaking the dormancy of seeds from the endangered forest tree T. baccata L. via zygotic embryo culture. Embryos isolated from 100% sterile seeds were cultured on DCR medium that contains sucrose (30 g/l), agar (8 g/l), and activated charcoal (5 g/l), fortified with different concentrations of Plant Growth Regulators (PGRs), and held at a temperature of 25 ± 2 ºC in a growth room. The results revealed that the in vitro embryo germination percentage was mostly affected by gibberellic acid (GA3) and thidiazuron (TDZ). Among the nine treatments, the treatments with 0.5 mg/l TDZ and 1 mg/l GA3 showed the highest germination (100%), while the other treatments all increased the germination percentages significantly compared to the control (37.5%). The 1/2 DCR medium with the addition of 0.1 mg/l indole-3-butyric acid (IBA) resulted in the highest rooting ratio (94%). However, the greatest root and hypocotyl elongation (59.37 ± 3.77 and 62.75 ± 4.43 mm, respectively) occurred when seedlings were cultured on 1/2 DCR medium containing 0.5 mg/l BA. Plantlets were transplanted into plastic pots containing an autoclaved garden soil, sand, and vermiculite mixture (1:1:1) and held at a temperature of 25 ± 2 ºC in a growth room for 4 weeks before being transplanted into the greenhouse. These results indicated that the protocol developed during the current study will be useful to overcome seed dormancy and for multiplication and conservation of the species T. baccata L.

scholarly journals Reproductive Characteristics of the Selected Cocoa (Theobroma cacao L.) Clones after Regenerated from the Somatic Embryogenesis Culture

2021 ◽  
Vol 18 (24) ◽  
pp. 1406
Author(s):  
Gibson Entuni ◽  
Hollena Nori ◽  
Rebicca Edward ◽  
Ahmad Kamil bin Mohammad Jaafar
Keyword(s):  
Somatic Embryogenesis ◽  
Reproductive Cycle ◽  
Embryo Culture ◽  
Zygotic Embryo ◽  
Reproductive Organ ◽  
Immature Zygotic Embryo ◽  
Reproductive Characteristics ◽  
Zygotic Embryo Culture ◽  
Typical Period ◽  
Immature Zygotic Embryo Culture

This study was conducted to evaluate the reproductive characteristics of 4 elite cocoa clones (MCBC1, PBC230, KKM22 and KKM4) propagated via somatic embryogenesis culture. From the findings, all clones have similar reproductive characteristics with clones from conventional grafted. However, only KKM4 clone from immature zygotic embryo culture produced the shortest staminode to style distance of 1.83 mm. This consequently influenced flower stability by reducing the efficiency of pollination by insects. It was found that this clone also has the highest number of flowers drop after anthesis (5 flowers) and lowest production of cherelle (5 cherelles). Further observation revealed that floral development from first bud visible (BBCH51) to flower anthesis (BBCH68) of all clones took around 31 days. These cocoa flowers which remained receptive soon after anthesis at 10 am (day-31) until the next day (day-32) suggesting 2 days’ period of receptivity. HIGHLIGHTS It is crucial to assess the presence of off-type characteristics in the reproductive organ structure such as the distance between staminode to style, period of reproductive cycle and stigmatic receptivity of cocoa clones regenerated from somatic embryogenesis The converging and parallel type of staminode to style distances are the ideal flower spatial arrangements for the optimal pollination in cocoa plant compared to splay type Only KKM4 clone propagated from immature zygotic embryo culture showed variation in the distance between staminode to style distance and this caused pollination failure by insect which then consequently caused minimum cherelle production All regenerated cocoa clones observed with typical period of the reproductive cycle and stigmatic receptivity GRAPHICAL ABSTRACT

scholarly journals In vitro culture from mature seeds of Passiflora species

2004 ◽  
Vol 61 (1) ◽  
pp. 108-113 ◽  
Author(s):  
Flavia Guzzo ◽  
Stefania Ceoldo ◽  
Filippo Andreetta ◽  
Marisa Levi
Keyword(s):  
In Vitro Culture ◽  
Fruit Juice ◽  
Zygotic Embryo ◽  
South American ◽  
Zygotic Embryo Culture ◽  
In The Wild ◽  
American Tropics ◽  
Mature Seeds ◽  
Continuous Source

The genus Passiflora comprises hundred species, mainly native of the South American tropics and rainforests, which are grouped into 21 subgenera. Some species are widely studied for their economic importance and are chiefly cultivated for production of fruit juice. To obtain a continuous source of material for a screening of secondary metabolites, zygotic embryo culture was attempted for 62 Passiflora species, starting from seeds mainly collected in the wild. Twenty nine of these species produced calli, which had very different growth rates. Plants were successfully regenerated from calli of 13 different species. For 25 of the responsive species this is the first report of in vitro culture.

scholarly journals Factors affecting developmental competence of equine oocytes after intracytoplasmic sperm injection

Reproduction ◽  
2004 ◽  
Vol 127 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Y H Choi ◽  
L B Love ◽  
D D Varner ◽  
K Hinrichs
Keyword(s):  
Embryo Culture ◽  
Intracytoplasmic Sperm Injection ◽  
Room Temperature ◽  
Culture Media ◽  
Developmental Competence ◽  
Glucose Medium ◽  
Factors Affecting ◽  
Nuclear Maturation ◽  
High Glucose Medium

This study was conducted to evaluate the effect of initial cumulus morphology (expanded or compact) and duration of in vitro maturation (24, 30 or 42 h) on the developmental competence of equine oocytes after intracytoplasmic sperm injection (ICSI). The effect of manipulation temperature (room temperature vs 37 °C) at the time of ICSI and concentration of glucose (0.55 vs 5.5 mM) during embryo culture was also investigated. The nuclear maturation rates of expanded (Ex) oocytes were significantly (P < 0.001) higher than those of compact (Cp) oocytes at all maturation times (61–72 vs 23–25% respectively). Forty-eight hours after ICSI of mature Ex oocytes, the rate of cleavage with normal nuclei was significantly (P < 0.05) higher for oocytes matured for 24 h than for those matured for 30 or 42 h (73 vs 57–59% respectively). For Cp oocytes, the morphologic cleavage rates for oocytes matured for 30 h were significantly higher (P < 0.05) than for those matured for 24 or 42 h (86 vs 55–61% respectively). The overall proportion of embryos having more than four normal nuclei at 48 h culture was significantly higher (P < 0.05) for Cp than for Ex oocytes. Manipulation temperature did not affect development of embryos from Ex or Cp oocytes at 96 h after ICSI. Culture in high-glucose medium significantly increased morphologic cleavage of Cp, but not Ex, oocytes (P < 0.05). Embryos from Cp oocytes had a significantly higher average nucleus number after 96-h culture than did embryos from Ex oocytes. These data indicate that developmental competence differs between Ex and Cp equine oocytes, and is differentially affected by the duration of maturation and by composition of embryo culture media.

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