High-frequency adventitious shoot bud induction and shoot elongation of Chile pepper (Capsicúm annuum L.)

2006 ◽  
Vol 42 (4) ◽  
pp. 341-344 ◽  
Author(s):  
P. G. Golegaonkar ◽  
A. S. Kantharajah
Keyword(s):  
Capsicum Annuum ◽  
High Frequency ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Chile Pepper ◽  
Shoot Bud ◽  
Capsicum Annuum L ◽  
Bud Induction ◽  
Adventitious Shoot Bud

In vitro regeneration in chile pepper (Capsicum annuum L.) from ?half-seed explants?

1996 ◽  
Vol 20 (3) ◽  
pp. 287-293 ◽  
Author(s):  
Marla L. Binzel ◽  
N. Sankhla ◽  
S. Joshi ◽  
D. Sankhla
Keyword(s):  
Capsicum Annuum ◽  
In Vitro Regeneration ◽  
Chile Pepper ◽  
Capsicum Annuum L ◽  
Half Seed

scholarly journals 893 PB 499 HIGH FREQUENCY REGENERATION FROM THE LEAF OF CAPSICUM ANNUUM L. CV. CHUNGHONG

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 561g-562
Author(s):  
Young Goel Shon ◽  
Joong Choon Park ◽  
Byoung Ryong Jeong
Keyword(s):  
Capsicum Annuum ◽  
Growth Regulators ◽  
High Frequency ◽  
Agar Medium ◽  
Multiple Shoots ◽  
Pepper Plant ◽  
Glass Bottle ◽  
Regeneration Frequency ◽  
Capsicum Annuum L ◽  
Better Than

Effect of combination and concentration of growth regulators on the regeneration of pepper plant from different explant tissues was studied. Seedlings were grown aseptically in 400 ml glass bottles containing MS agar medium at 26±2C under a 16 h·d-1 photoperiod (2000 lux, florescent lamps). Explants taken from 4 week-old seedlings were cultured under these conditions on 40 ml of MS agar (8 g·liter-1) medium containing 3 g·liter-1 sucrose in a 400 ml glass bottle. Primary and subsequent leaves attached to petiole regenerated better than cotyledon and hypocotyl. Among the combinations of different concentrations of cytokinin and auxin added in the medium, a combination of 5 μM IAA with either 10 μM zeatin or 10 μM BA gave the best regeneration. With these combinations, regeneration frequency of multiple shoots from the primary and subsequent leaves was greater than 70%. Regenerated shoots rooted readily in MS agar medium containing 3 g·liter-1 sucrose and 0.5 μM NAA.

Sign in / Sign up

Export Citation Format

Share Document