Optimizing Agrobacterium-mediated transformation of grapevine

2006 ◽  
Vol 42 (3) ◽  
pp. 220-227 ◽  
Author(s):  
Z. T. Li ◽  
S. Dhekney ◽  
M. Dutt ◽  
M. Aman ◽  
J. Tattersall ◽  
...  
Keyword(s):  
Agrobacterium Mediated Transformation

Agrobacterium-Mediated Transformation Of Plant Cells

2018 ◽  
Author(s):  
Christine Kronfoth ◽  
◽  
Peter Grayson ◽  
Keyword(s):  
Plant Cells ◽  
Agrobacterium Mediated Transformation

scholarly journals Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Transformation Rate ◽  
Suspension Cell Culture ◽  
Citrus Reticulata ◽  
Agrobacterium Mediated Transformation ◽  
Solid Media

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

Agrobacterium-mediated transformation of American chestnut (Castanea dentata (Marsh.) Borkh.) somatic embryos

2005 ◽  
Vol 84 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Linda D. Polin ◽  
Haiying Liang ◽  
Ronald E. Rothrock ◽  
Mutsumi Nishii ◽  
Deborah L. Diehl ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Castanea Dentata ◽  
American Chestnut ◽  
Agrobacterium Mediated Transformation

Agrobacterium mediated transformation of chickpea ( Cicer arietinum L.) embryo axes

2000 ◽  
Vol 19 (3) ◽  
pp. 235-240 ◽  
Author(s):  
K. V. Krishnamurthy ◽  
K. Suhasini ◽  
A. P. Sagare ◽  
M. Meixner ◽  
A. de Kathen ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Agrobacterium Mediated Transformation ◽  
Cicer Arietinum L ◽  
Embryo Axes

scholarly journals Transcriptomic Analysis of Arabidopsis Seedlings in Response to an Agrobacterium-Mediated Transformation Process

2018 ◽  
Vol 31 (4) ◽  
pp. 445-459 ◽  
Author(s):  
Kaixuan Duan ◽  
Christopher J. Willig ◽  
Joann R. De Tar ◽  
William G. Spollen ◽  
Zhanyuan J. Zhang
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Defense Response ◽  
Time Course ◽  
Basic Research ◽  
Defense Responses ◽  
Transformation Process ◽  
Host Responses ◽  
Nucleic Acid Metabolism ◽  
Agrobacterium Strain ◽  
Agrobacterium Mediated Transformation

Agrobacterium tumefaciens is a plant pathogen that causes crown gall disease. This pathogen is capable of transferring the T-DNA from its Ti plasmid to the host cell and, then, integrating it into the host genome. To date, this genetic transformation ability has been harnessed as the dominant technology to produce genetically modified plants for both basic research and crop biotechnological applications. However, little is known about the interaction between Agrobacterium tumefaciens and host plants, especially the host responses to Agrobacterium infection and its associated factors. We employed RNA-seq to follow the time course of gene expression in Arabidopsis seedlings infected with either an avirulent or a virulent Agrobacterium strain. Gene Ontology analysis indicated many biological processes were involved in the Agrobacterium-mediated transformation process, including hormone signaling, defense response, cellular biosynthesis, and nucleic acid metabolism. RNAseq and quantitative reverse transcription-polymerase chain reaction results indicated that expression of genes involved in host plant growth and development were repressed but those involved in defense response were induced by Agrobacterium tumefaciens. Further analysis of the responses of transgenic Arabidopsis lines constitutively expressing either the VirE2 or VirE3 protein suggested Vir proteins act to enhance plant defense responses in addition to their known roles facilitating T-DNA transformation.

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