Inheritance of transgenes in transgenic tall fescue (Festuca arundinacea schreb.)

2003 ◽  
Vol 39 (3) ◽  
pp. 277-282 ◽  
Author(s):  
Z. Y. Wang ◽  
J. Bell ◽  
Y. X. Ge ◽  
D. Lehmann
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Transgenic Tall Fescue

Field performance of transgenic tall fescue (Festuca arundinacea Schreb.) plants and their progenies

2003 ◽  
Vol 107 (3) ◽  
pp. 406-412 ◽  
Author(s):  
Z. Y. Wang ◽  
M. Scott ◽  
J. Bell ◽  
A. Hopkins ◽  
D. Lehmann
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Field Performance ◽  
Transgenic Tall Fescue

scholarly journals Conferred Resistance to an Acetolactate Synthase-inhibiting Herbicide in Transgenic Tall Fescue (Festuca arundinacea Schreb.)

HortScience ◽  
2009 ◽  
Vol 44 (5) ◽  
pp. 1254-1257 ◽  
Author(s):  
Hiroko Sato ◽  
Tadashi Takamizo ◽  
Tsutomu Shimizu ◽  
Kiyoshi Kawai ◽  
Koichiro Kaku
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Leaf Tissue ◽  
Acetolactate Synthase ◽  
Branched Chain Amino Acids ◽  
Wild Type ◽  
Efficient Tool ◽  
Herbicide Resistant ◽  
Branched Chain ◽  
Transgenic Tall Fescue

Herbicide-resistant turfgrass can be an efficient tool that will allow easier turf maintenance. Acetolactate synthase (ALS) is the first common enzyme in the biosynthetic pathways leading to the branched-chain amino acids, and amino acid substitutions in ALS have been known to confer resistance to ALS-inhibiting herbicides. A two-point mutated rice ALS gene [OsALS (dm)] has been shown to confer strong resistance to bispyribac-sodium (BS), an ALS-inhibiting herbicide. In this study, we introduced into turf-type tall fescue (Festuca arundinacea Schreb.) the OsALS (dm) gene by using Agrobacterium-mediated transformation for conferring herbicide resistance. Stable integration of the transgene was confirmed by Southern blot analysis. Transgenic and wild-type plants were sprayed on the leaves with herbicide containing BS; approximately half of the transgenic plants were unaffected by the treatment and showed resistance to the herbicide, whereas the wild-type plants died. ALS activity in the leaf tissue of transgenic-resistant plants incubated with BS was almost equivalent to that in wild-type plants without BS and was higher than in wild-type plants incubated with BS. These indicate that the transgenic-resistant plants actively produced OsALS (dm) protein under herbicide treatment. This is the first report of herbicide-resistant transgenic tall fescue after introduction of a mutated ALS gene.

Transgenic down-regulation of caffeic acid O-methyltransferase (COMT) led to improved digestibility in tall fescue (Festuca arundinacea)

2004 ◽  
Vol 31 (3) ◽  
pp. 235 ◽  
Author(s):  
Lei Chen ◽  
Chung-Kyoon Auh ◽  
Paul Dowling ◽  
Jeremey Bell ◽  
Deane Lehmann ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Lignin Content ◽  
Microprojectile Bombardment ◽  
Lignin Biosynthesis ◽  
Suspension Cells ◽  
Dry Matter Digestibility ◽  
Transgenic Lines ◽  
Transgenic Tall Fescue

Dry matter digestibility is one of the most important characteristics of forage. The major constraint on ruminant digestion of forage cell walls is lignin. Sequences of cDNA encoding a key lignin biosynthetic enzyme, caffeic acid O-methyltransferase (COMT), was cloned from the widely grown monocot forage species tall fescue (Festuca arundinacea Schreb.). Enzymatic properties of recombinant COMT protein expressed in E. coli were determined using six substrates. The preferred substrates for tall fescue recombinant COMT were 5-hydroxyferulic acid and caffeoyl aldehyde. Transgenic tall fescue plants carrying either sense or antisense COMT gene constructs were obtained by microprojectile bombardment of single-genotype-derived embryogenic suspension cells. Consistent and closely related molecular and biochemical data demonstrated that two co-suppressed transgenic lines were down-regulated in their lignin biosynthesis. These COMT down-regulated transgenic tall fescue plants showed substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased (9.8-10.8%) digestibility.

Expression of a sulphur-rich sunflower albumin gene in transgenic tall fescue (Festuca arundinacea Schreb.) plants

2001 ◽  
Vol 20 (3) ◽  
pp. 213-219 ◽  
Author(s):  
Z.Y. Wang ◽  
X.D. Ye ◽  
J. Nagel ◽  
I. Potrykus ◽  
G. Spangenberg
Keyword(s):  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Albumin Gene ◽  
Sunflower Albumin ◽  
Transgenic Tall Fescue

scholarly journals Co-expression of the Arabidopsis SOS genes enhances salt tolerance in transgenic tall fescue (Festuca arundinacea Schreb.)

PROTOPLASMA ◽  
2013 ◽  
Vol 251 (1) ◽  
pp. 219-231 ◽  
Author(s):  
Dong-Mei Ma ◽  
Wei-Rong Xu ◽  
Hui-Wen Li ◽  
Feng-Xia Jin ◽  
Ling-Na Guo ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Transgenic Tall Fescue
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