Factors affecting growth of cell suspension cultures of hypericum perforatum L. (St. John's wort) and production of hypericin

2002 ◽  
Vol 38 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Harsh Pal Bais ◽  
Travis S. Walker ◽  
Janeen J. McGrew ◽  
Jorge M. Vivanco
Keyword(s):  
Cell Suspension ◽  
Hypericum Perforatum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Factors Affecting ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L

Determination of Hypericin in St. John's Wort (Hypericum perforatum L.) extracts using HPLC-ED

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
E Sofic ◽  
A Copra Janicijevic ◽  
M Maksimovic ◽  
I Tahirovic ◽  
L Klepo ◽  
...  
Keyword(s):  
Hypericum Perforatum ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L

Effect of herbicides on St John's wort (Hypericum perforatum L.)

1991 ◽  
Vol 31 (4) ◽  
pp. 499 ◽  
Author(s):  
MH Campbell ◽  
BR Milne ◽  
JJ Dellow ◽  
HI Nicol
Keyword(s):  
Hypericum Perforatum ◽  
New South ◽  
Arable Land ◽  
Ground Cover ◽  
Aerial Application ◽  
St John’S Wort ◽  
Strong Trend ◽  
St John's Wort ◽  
South Wales ◽  
Hypericum Perforatum L

The effect of type of herbicide and time and rate of application on the reduction in ground cover of St John's wort (Hypericum perforatum L.) was determined at Orange, New South Wales. In January, April, July and November 1988, 8 herbicide treatments including the currently used glyphosate and picloram + 2,4-D were applied to vigorously growing H. perforaturn, and the reduction in percentage ground cover was recorded in December 1989. Ineffective herbicides (a.i./ha) were tebuthiuron, 0.8-6.4 kg; metsulfuron, 5-20 g; and paraquat + diquat, 0.4 + 0.5 kg. The addition of metsulfuron (2.5 g a.i./ha) to glyphosate (0.9 and 1.8 kg a.i./ha) did not increase the effectiveness of the latter. Effective herbicides (kg a.i./ha) were triclopyr + picloram, 0.6 + 0.2; picloram + 2,4-D, 0.2 + 0.8; glyphosate, 1.8; and triclopyr, 1.92. There was a strong trend for these herbicides to be more effective in January and November than in April and July. Based on price, effectiveness and selectivity, triclopyr + picloram would be preferred to the other herbicides for boom and spot spraying, and glyphosate would be the only herbicide suitable for aerial application prior to sowing improved pastures on non-arable land.

EXTRACTION OF HYPERFORIN AND HYPERICIN FROM ST. JOHN'S WORT (HYPERICUM PERFORATUM L.) WITH DIFFERENT SOLVENTS

2011 ◽  
Vol 35 (2) ◽  
pp. 222-235 ◽  
Author(s):  
DÁNIEL COSSUTA ◽  
TÜNDE VATAI ◽  
MÁRIA BÁTHORI ◽  
JUDIT HOHMANN ◽  
TIBOR KEVE ◽  
...  
Keyword(s):  
Hypericum Perforatum ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L

scholarly journals Extraction of resinoids from St. John's wort (Hypericum perforatum L): I. Efficiency and optimization of extraction

2002 ◽  
Vol 56 (2) ◽  
pp. 54-59 ◽  
Author(s):  
Dragan Milenovic ◽  
Vlada Veljkovic ◽  
Branimir Todorovic ◽  
Miomir Stankovic
Keyword(s):  
Plant Material ◽  
Hypericum Perforatum ◽  
Ethanol Solution ◽  
Operating Conditions ◽  
Solvent Ratio ◽  
Total Extract ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L ◽  
Full Factorial

The extraction of resinoids from St. John's wort (Hypericum perforatum L) was studied in a series of two papers. In the first part, the effects of the operating conditions on the yield of resinoids (total extract) were analyzed, while the mathematical models of extraction kinetics were compared in the second one. The extraction was carried out using an aqueous solution of ethanol (70 and 95 % v/v) at a hydromodulus (plant material to solvent ratio, w/v) of 1:5 or 1:10. The plant material was disintegrated and divided into three fractions (mean particle size: 0.23, 0.57 and 1.05 mm). The temperature was 25, 50 or about 80?C (boiling temperature). A higher yield of resinoids was obtained when the plant material of greater disintegration degree (0.23 mm) was treated with 70% v/v aqueous ethanol solution at higher hydromoduli (1:10) and temperatures (80?C). The effects of the operating factors on the yield of resinoids were estimated by using both the full factorial experimental plan 24 and artificial neuronic networks (ANN) of 3-4-1 topology. Of the two methods, the ANN one was found to be advantageous because of its capability of estimating the yield of resinoids in the whole range of the applied operating conditions.

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