Intermittent administration of human parathyroid fragment (hPTH 1-37) on calcium and phosphorus homeostasis and bone markers in resting horses: a preliminary study

2005 ◽  
Vol 2 (4) ◽  
pp. 245-251
Author(s):  
I Vervuert ◽  
K Von Scheidt ◽  
S Winkelsett ◽  
WG Forssmann ◽  
M Coenen
Keyword(s):  
Bone Turnover ◽  
Type I Collagen ◽  
Bone Markers ◽  
Type I ◽  
Post Injection ◽  
Test Protocol ◽  
Inorganic P ◽  
Skeletal Injury ◽  
Identical Test ◽  
Carboxyterminal Telopeptide

AbstractParathyroid hormone (PTH) has been shown to have anabolic and catabolic effects on the skeleton, and in young racing horses the anabolic effects of PTH on bone might be of great importance in reducing the risk of skeletal injury during race training. The aim of this pilot study was to elucidate the effects of intermittent exogenous application of human parathyroid fragment (hPTH 1-37) on calcium homeostasis and bone turnover in resting horses. Five horses were used in this study. Horses were treated subcutaneously with hPTH (hPTH 1-37, 0.5 μg kg−1 BW) daily at 600 h over a period of 28 days. A foregoing control trial was conducted under the identical test protocol without hPTH application. Blood samples were taken at defined times for the control and hPTH treatment to analyze ionized Ca (Ca++, AVL), total Ca (AAS), inorganic Pi (flame photometry), intact PTH (RIA), osteocalcin (ELISA) and ICTP (carboxyterminal telopeptide of type-I collagen, RIA). Eight hours after hPTH application, blood ionized Ca++ (days 1 and 28) and plasma Pi (days 1–28) increased significantly in comparison to the control. At day 1, 8 h after hPTH application, the decrease in intact plasma PTH was more pronounced after hPTH treatment than in the control, and plasma PTH levels were higher after treatment at day 14 (16 h post-injection, P<0.05), day 18 (16 h post-injection, ns) and day 24 (0 h post-injection). There were no treatment-related differences in total plasma Ca and bone markers. Intermittent PTH administration in healthy horses affected Ca and P homeostasis as well as endogenous PTH secretion, but bone turnover was not affected during the treatment period of 28 days.

Diurnal variation in concentrations of various markers of bone metabolism in growing female goats and sheep

2003 ◽  
Vol 77 (2) ◽  
pp. 197-203 ◽  
Author(s):  
A. Liesegang ◽  
M.-L. Sassi ◽  
J. Risteli
Keyword(s):  
Type I Collagen ◽  
Bone Markers ◽  
Degradation Products ◽  
Bone Marker ◽  
Type I ◽  
Blood Samples ◽  
Bone Specific Alkaline Phosphatase ◽  
Growing Goats ◽  
Turn Over ◽  
Carboxyterminal Telopeptide

AbstractTwelve 6-month-old growing female goats and sheep were used in this study. Blood samples were obtained in the morning before goats and sheep were given food and then at 2-h intervals for 24 h (part I). This procedure was repeated 2 weeks later (part II). Concentrations of osteocalcin (OC), activities of total (tAP) and bone-specific alkaline phosphatase (bAP), degradation products of C-terminal telopeptide of type-I collagen (CrossLaps™ CL), and carboxyterminal telopeptide of type-I collagen (ICTP) were measured in serum.In both parts of the study, all bone marker concentrations were significantly higher in goats than in sheep. The OC concentrations in goats increased in the late afternoon/evening and decreased thereafter to reach values similar to those obtained at the beginning. The ICTP concentrations in goats slowly decreased until 14:00 h, increased, and decreased again. The concentrations in sheep decreased continuously but not significantly, towards the morning sampling. The CL concentrations increased in both sheep and goats during the night but at 06:00 h started to decrease to levels found at the beginning of testing. The bAP activities decreased in goats from 20:00 to 22:00 h. Changes in the concentrations of bone markers were mainly observed in goats of this study. As documented for bone resorption and formation in other species, circadian rhythms were evident for concentrations of ICTP, CL, bAP and OC. The present study indicates that growing goats may have a physiologically higher bone turn-over than growing sheep, because the bone marker concentrations were always higher.

scholarly journals Sex- and Age-Specific Reference Curves for Serum Markers of Bone Turnover in Healthy Children from 2 Months to 18 Years

2006 ◽  
Vol 92 (2) ◽  
pp. 443-449 ◽  
Author(s):  
Markus Rauchenzauner ◽  
Andrea Schmid ◽  
Peter Heinz-Erian ◽  
Klaus Kapelari ◽  
Gerda Falkensammer ◽  
...  
Keyword(s):  
Bone Formation ◽  
Type I Collagen ◽  
Bone Markers ◽  
Collagen Degradation ◽  
Specific Reference ◽  
Type I ◽  
Healthy Children ◽  
Pubertal Stage ◽  
Reference Curves ◽  
Carboxyterminal Telopeptide

Abstract Introduction: This study aimed to establish sex- and age-specific reference curves enabling the calculation of z-scores and to examine correlations between bone markers and anthropometric data. Methods: Morning blood samples were obtained from 572 healthy children and adolescents (300 boys) aged 2 months to 18 yr. Height, weight, and pubertal stage were recorded. Serum osteocalcin (OC), bone-specific alkaline phosphatase (BALP), type-1 collagen degradation markers [carboxyterminal telopeptide region of type I collagen (ICTP), carboxyterminal telopeptide α1 chain of type I collagen (CTX)], and tartrate-resistant acid phosphatase (TRAP5b) were measured. Cross-sectional centile charts were created for the 3rd, 50th, and 97th centiles. Results: Apart from TRAP5b, all bone markers were nonnormally distributed, requiring logarithmic (BALP, OC, ICTP) or square root (CTX) transformation. Back-transformed centile curves for age and sex are presented for practical use. All bone markers varied with age and pubertal stage (P &lt; 0.001). Significant correlations were found between sd score (SDS) for bone formation markers BALP and OC (r = 0.13; P = 0.004), SDS for collagen degradation markers ICTP and CTX (r = 0.14; P = 0.002), and SDS for the phosphatases (r = 0.34, P &lt; 0.001). Height and weight SDS correlated weakly with some bone marker SDS, particularly with lnBALP SDS (r = 0.20 and 0.24, respectively; both P &lt; 0.001). Conclusion: This study provides reference curves for OC, BALP, CTX, ICTP, and TRAP5b in healthy children. Taller and heavier individuals for age had greater bone marker concentrations, likely reflecting greater growth velocity. SDS for markers of bone formation, collagen degradation, and phosphatases were each independently correlated, suggesting they derive from the same biological processes. The possibility of calculating SDS will facilitate monitoring of antiresorptive therapy or disease progression in children with metabolic bone disease.

scholarly journals Bone turnover and metabolism in paediatric patients with inflammatory bowel disease treated with systemic glucocorticoids

2008 ◽  
Vol 159 (6) ◽  
pp. 693-698 ◽  
Author(s):  
Marianne K Vihinen ◽  
Kaija-Leena Kolho ◽  
Merja Ashorn ◽  
Matti Verkasalo ◽  
Taneli Raivio
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bone Formation ◽  
Bone Turnover ◽  
Type I Collagen ◽  
Bone Markers ◽  
Type I ◽  
Glucocorticoid Treatment ◽  
Systemic Glucocorticoid ◽  
Igf I ◽  
Inflammatory Bowel

ObjectiveWe investigated circulating markers of bone turnover before and during systemic glucocorticoid treatment in paediatric patients with inflammatory bowel disease (IBD).MethodsTwenty-two children (mean age, 12.3 years) with IBD necessitating peroral steroid therapy were studied, with special reference to bone formation and resorption markers amino-terminal type I collagen propeptide (PINP) and carboxyterminal telopeptide of type I collagen (ICTP) respectively. In addition, GH-related IGF-I and sex hormone-binding protein (SHBG) were measured. Bone markers were analyzed at the initiation of the glucocorticoid treatment, at 2 and 5 weeks thereafter and at 1 month following the withdrawal of the steroid. Control group comprised 22 IBD patients in remission.ResultsPINP and IGF-I were already lower before glucocorticoid treatment serum in children with active IBD as compared with control children with IBD in remission (median PINP 271 vs 535 μg/l, P<0.05; IGF-I 23 vs 29 nmol/l, P<0.05). After 2 weeks of glucocorticoid treatment serum PINP levels had declined further, from 271 to 163 μg/l (P<0.001), serum ICTP from 14.2 to 9.6 μg/l (P<0.001), and SHBG from 54 to 35 nmol/l (P<0.001) respectively. By contrast, serum IGF-I increased from 23 to 37 nmol/l (P<0.001). One month after the withdrawal of the glucocorticoid, all bone markers restored to levels similar to the controls.ConclusionsBone formation in children with active IBD appears compromised and systemic glucocorticoid treatment further suppresses bone turnover. After the cessation of the glucocorticoid the bone markers show immediate improvement.

scholarly journals Interlaboratory Variation of Biochemical Markers of Bone Turnover

2001 ◽  
Vol 47 (8) ◽  
pp. 1443-1450 ◽  
Author(s):  
Markus J Seibel ◽  
Matthias Lang ◽  
Wolf-Jochen Geilenkeuser
Keyword(s):  
Bone Turnover ◽  
Biochemical Markers ◽  
Type I Collagen ◽  
Bone Markers ◽  
Type I ◽  
Urinary Hydroxyproline ◽  
Bone Specific Alkaline Phosphatase ◽  
Practical Concern ◽  
Markers Of Bone Turnover ◽  
Biochemical Bone Markers

Abstract Background: Biochemical markers of bone metabolism are used to assess skeletal turnover, but the variability of marker assays is still an issue of practical concern. We describe the results of an international proficiency testing program for biochemical bone markers among clinical laboratories. Methods: Two serum and two urine pools (normal and increased marker concentrations) were sent on dry ice to 79 laboratories for analysis within 2 weeks of receipt. Results: Data were submitted by 73 laboratories. The within-method interlaboratory CVs (CVILs) were as follows: serum bone-specific alkaline phosphatase (n = 47 laboratories), 16–48%; serum osteocalcin (n = 31), 16–42%; urinary free deoxypyridinoline (n = 30), 6.4–12%; urinary total deoxypyridinoline and pyridinoline (n = 29), 27–28%; urinary N-terminal cross-linked telopeptide of type I collagen (n = 10), 39%; serum C-terminal cross-linked telopeptide of type I collagen (ICTP; n = 8), 22–27%; urinary hydroxyproline (n = 13), 12%. Analytical results showed both systematic and nonsystematic deviations. In identical samples, results obtained for the same marker by the same method differed up to 7.3-fold. In urine-based assays, correction for urinary creatinine slightly increased CVs. Conclusion: Even with identical assays and methods, results for most biochemical markers of bone turnover differ markedly among laboratories.

scholarly journals Effects of different vibration frequencies on muscle strength, bone turnover and walking endurance in chronic stroke

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhenhui Yang ◽  
Tiev Miller ◽  
Zou Xiang ◽  
Marco Y. C. Pang
Keyword(s):  
Muscle Strength ◽  
Bone Turnover ◽  
Intervention Program ◽  
Type I Collagen ◽  
Chronic Stroke ◽  
Medium Effect ◽  
Whole Body ◽  
Type I ◽  
Leg Muscle ◽  
Walking Endurance

AbstractThis randomized controlled trial aimed to evaluate the effects of different whole body vibration (WBV) frequencies on concentric and eccentric leg muscle strength, bone turnover and walking endurance after stroke. The study involved eighty-four individuals with chronic stroke (mean age = 59.7 years, SD = 6.5) with mild to moderate motor impairment (Fugl-Meyer Assessment lower limb motor score: mean = 24.0, SD = 3.5) randomly assigned to either a 20 Hz or 30 Hz WBV intervention program. Both programs involved 3 training sessions per week for 8 weeks. Isokinetic knee concentric and eccentric extension strength, serum level of cross-linked N-telopeptides of type I collagen (NTx), and walking endurance (6-min walk test; 6MWT) were assessed at baseline and post-intervention. An intention-to-treat analysis revealed a significant time effect for all muscle strength outcomes and NTx, but not for 6MWT. The time-by-group interaction was only significant for the paretic eccentric knee extensor work, with a medium effect size (0.44; 95% CI: 0.01, 0.87). Both WBV protocols were effective in improving leg muscle strength and reducing bone resorption. Comparatively greater improvement in paretic eccentric leg strength was observed for the 30 Hz protocol.

scholarly journals Serum levels of bone formation and resorption markers in relation to vitamin D status in professional gymnastics and physically active men during upper and lower body high-intensity exercise

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Jan Mieszkowski ◽  
Andrzej Kochanowicz ◽  
Elżbieta Piskorska ◽  
Bartłomiej Niespodziński ◽  
Joanna Siódmiak ◽  
...  
Keyword(s):  
Vitamin D ◽  
Bone Formation ◽  
Bone Turnover ◽  
Bone Turnover Markers ◽  
Type I Collagen ◽  
Serum Levels ◽  
Type I ◽  
Vitamin D Metabolites ◽  
Physically Active ◽  
Turnover Markers

Abstract Purpose/introduction To compare serum levels of bone turnover markers in athletes and non-athletes, and to evaluate the relationship between serum levels of vitamin D metabolites and exercise-induced changes in biomarker levels. Methods Sixteen elite male artistic gymnasts (EG; 21.4 ± 0.8 years-old) and 16 physically active men (the control group, PAM; 20.9 ± 1.2 years-old) performed lower and upper body 30-s Wingate anaerobic tests (LBWT and UBWT, respectively). For biomarker analysis, blood samples were collected before, and 5 and 30 min after exercise. Samples for vitamin D levels were collected before exercise. N-terminal propeptide of type I collagen (PINP) was analysed as a marker of bone formation. C-terminal telopeptide of type I collagen (CTX) was analysed as a marker of bone resorption. Results UBWT fitness readings were better in the EG group than in the PAM group, with no difference in LBWT readings between the groups. UBWT mean power was 8.8% higher in subjects with 25(OH)D3 levels over 22.50 ng/ml and in those with 24,25(OH)2D3 levels over 1.27 ng/ml. Serum CTX levels increased after both tests in the PAM group, with no change in the EG group. PINP levels did not change in either group; however, in PAM subjects with 25(OH)D3 levels above the median, they were higher than those in EG subjects. Conclusion Vitamin D metabolites affect the anaerobic performance and bone turnover markers at rest and after exercise. Further, adaptation to physical activity modulates the effect of anaerobic exercise on bone metabolism markers.

scholarly journals Diurnal Rhythms of Bone Turnover Markers in Three Ethnic Groups

2016 ◽  
Vol 101 (8) ◽  
pp. 3222-3230 ◽  
Author(s):  
Jean Redmond ◽  
Anthony J. Fulford ◽  
Landing Jarjou ◽  
Bo Zhou ◽  
Ann Prentice ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Bone Metabolism ◽  
Ethnic Groups ◽  
Bone Turnover Markers ◽  
Type I Collagen ◽  
Diurnal Rhythms ◽  
Type I ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D ◽  
Turnover Markers

Context: Ethnic groups differ in fragility fracture risk and bone metabolism. Differences in diurnal rhythms (DRs) of bone turnover and PTH may play a role. Objective: We investigated the DRs of plasma bone turnover markers (BTMs), PTH, and 1,25(OH)2D in three groups with pronounced differences in bone metabolism and plasma PTH. Participants: Healthy Gambian, Chinese, and white British adults (ages 60–75 years; 30 per country). Interventions: Observational study with sample collection every 4 hours for 24 hours. Main Outcomes: Levels of plasma C-terminal telopeptide of type I collagen, procollagen type-1 N-propeptide, N-mid osteocalcin, bone alkaline phosphatase, PTH, and 1,25-dihydroxyvitamin D were measured. DRs were analyzed with random-effects Fourier regression and cross-correlation and regression analyses to assess associations between DRs and fasting and 24-hour means of BTMs and PTH. Results: Concentrations of BTMs, PTH, and 1,25-dihydroxyvitamin D were higher in Gambians compared to other groups (P &lt; .05). The DRs were significant for all variables and groups (P &lt; .03) and were unimodal, with a nocturnal peak and a daytime nadir for BTMs, whereas PTH had two peaks. The DRs of BTMs and PTH were significantly cross-correlated for all groups (P &lt; .05). There was a significant positive association between C-terminal telopeptide of type I collagen and PTH in the British and Gambian groups (P = .03), but not the Chinese group. Conclusions: Despite ethnic differences in plasma BTMs and PTH, DRs were similar. This indicates that alteration of rhythmicity and loss of coupling of bone resorption and formation associated with an elevated PTH in other studies may not uniformly occur across different populations and needs to be considered in the interpretation of PTH as a risk factor of increased bone loss.

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