Characterization of trait variations in progenies of Gossypium hirsutum transformed with the genomic DNA of Gossypium barbadense

2005 ◽  
Vol 2 (1) ◽  
pp. 39-44
Author(s):  
Du Xiong-Ming ◽  
Pan Zhao-E ◽  
Sun Jun-Ling ◽  
Zhou Zhong-Li ◽  
Pang Bao-Yin
Keyword(s):  
Gossypium Hirsutum ◽  
Upland Cotton ◽  
Genomic Dna ◽  
Agronomic Traits ◽  
Gossypium Barbadense ◽  
Wild Type ◽  
Ssr Loci ◽  
Transformation Experiment ◽  
Foreign Dna

AbstractFour variant lines, HB1, HB2, HB3 and HB4, were derived from progenies of upland cotton (Gossypium hirsutum) cultivar Yumian 17 that had been transformed with genomic DNA from sea island cotton, Gossypium barbadense, using the pollen tube pathway (PTP) method. They showed significant differences from the wild-type recipient in terms of fibre quality, lint percentage, boll weight and other agronomic traits. The four variant lines also differed from the wild-type recipient in the amplification products of two SSR loci, indicating that the DNA of G. barbadense had been introduced into the genome of the recipient and was inherited stably. On the other hand, the agronomic traits of another three lines (HB5, HB6 and HB7) derived from the same transformation experiment were identical to those of the wild-type recipient, indicating that G. barbadense DNA had not integrated into the genome of these lines. A combination of the analyses on agronomic traits and SSR markers indicated that transgenic upland cotton strains produced by the PTP method could become homozygous in only a few generations and the foreign DNA introduced during the transformation experiments could be inherited stably in the transgenic progenies.

scholarly journals Genetic mapping and transcriptomic characterization of a new fuzzless-tufted cottonseed mutant

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qian-Hao Zhu ◽  
Warwick Stiller ◽  
Philippe Moncuquet ◽  
Stuart Gordon ◽  
Yuman Yuan ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Agronomic Traits ◽  
Gene Families ◽  
Mutant Phenotype ◽  
Wild Type ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Flanking Region ◽  
Comparative Transcriptomic Analysis

Abstract Fiber mutants are unique and valuable resources for understanding the genetic and molecular mechanisms controlling initiation and development of cotton fibers that are extremely elongated single epidermal cells protruding from the seed coat of cottonseeds. In this study, we reported a new fuzzless-tufted cotton mutant (Gossypium hirsutum) and showed that fuzzless-tufted near-isogenic lines (NILs) had similar agronomic traits and a higher ginning efficiency compared to their recurrent parents with normal fuzzy seeds. Genetic analysis revealed that the mutant phenotype is determined by a single incomplete dominant locus, designated N5. The mutation was fine mapped to an approximately 250-kb interval containing 33 annotated genes using a combination of bulked segregant sequencing, SNP chip genotyping, and fine mapping. Comparative transcriptomic analysis using 0–6 days post-anthesis (dpa) ovules from NILs segregating for the phenotypes of fuzzless-tufted (mutant) and normal fuzzy cottonseeds (wild-type) uncovered candidate genes responsible for the mutant phenotype. It also revealed that the flanking region of the N5 locus is enriched with differentially expressed genes (DEGs) between the mutant and wild-type. Several of those DEGs are members of the gene families with demonstrated roles in cell initiation and elongation, such as calcium-dependent protein kinase and expansin. The transcriptome landscape of the mutant was significantly reprogrammed in the 6 dpa ovules and, to a less extent, in the 0 dpa ovules, but not in the 2 and 4 dpa ovules. At both 0 and 6 dpa, the reprogrammed mutant transcriptome was mainly associated with cell wall modifications and transmembrane transportation, while transcription factor activity was significantly altered in the 6 dpa mutant ovules. These results imply a similar molecular basis for initiation of lint and fuzz fibers despite certain differences.

Photosynthetic Rate, Stomatal Conductance and Leaf Area in Two Cotton Species (Gossypium barbadense and Gossypium hirsutum) and their Relation with Heat Resistance and Yield

1997 ◽  
Vol 24 (5) ◽  
pp. 693 ◽  
Author(s):  
Zhenmin Lu ◽  
Jiwei Chen ◽  
Richard G. Percy ◽  
Eduardo Zeiger
Keyword(s):  
Stomatal Conductance ◽  
Gossypium Hirsutum ◽  
Heat Resistance ◽  
Leaf Area ◽  
Photosynthetic Rate ◽  
Upland Cotton ◽  
Gossypium Barbadense ◽  
Lint Yield ◽  
Historical Series ◽  
Field And Laboratory Conditions

Gossypium barbadense L. (Pima) and Gossypium hirsutum L. (upland) cottons are the two major fibre producing species grown in the south-western United States, where lint yields are adversely affected by high temperatures. In these environments, heat-adapted upland cultivars show higher yields and heat resistance than advanced Pima cultivars. Recent studies with an historical series of commercial Pima cultivars have shown that increases in lint yield and heat resistance are tightly coupled to increases in stomatal conductance and photosynthetic rate, and to decreases in leaf area. In the present study, Pima S-6 and Pima S-7 (advanced Pima cultivars) and Deltapine 90 (advanced upland cultivar) were compared under field and laboratory conditions to determine whether the physiological and morphological gradients found in the Pima historical series extrapolate to upland cotton. In the field, Deltapine 90 showed 25–35% higher stomatal conductance, 35–50% higher photosynthetic rate and 45% smaller leaf area than Pima S-6. The higher photosynthetic rate and stomatal conductance of Deltapine 90 leaves were partially related to their sun-tracking ability. In gas exchange experiments that prevented sun-tracking, the two cultivars had comparable photosynthetic rate as a function of incident radiation, while stomatal conductance was higher in upland cotton. In the 25–35°C range, photosynthetic rate as a function of temperature remained nearly constant in both cultivars, and was higher in upland cotton at all temperatures. Stomatal conductance showed a strong temperature-dependence, and conductance value and the slope of the stomatal response to temperature were higher in Deltapine 90. In progeny from a cross between Deltapine 90 and Pima S-7, the segregation of stomatal conductance in F1 and F2 populations showed a clear genetic component. These results indicate that the differences in photosynthetic rate, stomatal conductance and leaf area associated with increases in lint yield and heat resistance in the Pima historical series are also evident in a comparison between advanced cultivars of upland and Pima cotton. Upland cotton could be used as a source of genetic variation for high stomatal conductance in Pima breeding programs.

Registration of GA R01-40-08, a Gossypium hirsutum Upland Cotton Germplasm Line with qFL-Chr.1 Introgressed from Gossypium barbadense Conferring Improved Fiber Length

2019 ◽  
Vol 13 (3) ◽  
pp. 406-410
Author(s):  
Nino Brown ◽  
Xinlian Shen ◽  
Edward L. Lubbers ◽  
Pawan Kumar ◽  
Jennifer McBlanchett ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Upland Cotton ◽  
Fiber Length ◽  
Gossypium Barbadense ◽  
Germplasm Line
Sign in / Sign up

Export Citation Format

Share Document