AFLP markers for genomic DNA fingerprinting in pigs

2004 ◽  
Vol 1 (1) ◽  
pp. 9-12
Author(s):  
Ren Jun ◽  
Huang Lu-Sheng ◽  
Gary Evens ◽  
Ai Hua-Shui ◽  
Gao Jun ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Genomic Dna ◽  
Aflp Markers

scholarly journals Genomic DNA fingerprinting by restriction fragment end labeling.

1995 ◽  
Vol 92 (12) ◽  
pp. 5572-5576 ◽  
Author(s):  
T. J. van Steenbergen ◽  
S. D. Colloms ◽  
P. W. Hermans ◽  
J. de Graaff ◽  
R. H. Plasterk
Keyword(s):  
Dna Fingerprinting ◽  
Restriction Fragment ◽  
Genomic Dna

scholarly journals RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN GENETICALLY RELATED ROSE CULTIVARS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 574d-574
Author(s):  
Sriyani Rajapakse ◽  
Albert Abbott ◽  
John Kelly ◽  
Robert Ballard
Keyword(s):  
Escherichia Coli ◽  
Restriction Fragment Length Polymorphism ◽  
Dna Fingerprinting ◽  
Genomic Dna ◽  
Genetic Relatedness ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Rflp Markers ◽  
High Degree ◽  
Restriction Fragment Length

The feasibility of using RFLP to distinguish genetically related Hybrid Tea rose cultivars for DNA `fingerprinting' was examined with a group of cultivars related to `Peace'. The following cultivars used in this study, `Chicago Peace', `Flaming Peace', `Climbing Peace' and `Lucky Piece', were derived from bud mutations (sports) of `Peace'. We also investigated two additional cultivars, `Perfume Delight' and `Garden Party', in which one of the parents for each was `Peace'. Genomic rose DNA probes, cloned in pUC8 plasmid of Escherichia coli, were hybridized with genomic DNA of these cultivars digested with different restriction enzymes. Although polymorphisms were observed among these related cultivars, only a few probe/enzyme combinations screened produced RFLPs due to the high degree of genetic relatedness of these cultivars. We have identified probes that can distinguish all of these related rose cultivars. This study demonstrates that RFLP markers can be used effectively in DNA `fingerprinting' of genetically related rose cultivars, eventhough the level of detectable polymorphism is quite low.

scholarly journals DNA FINGERPRINTING TECHNOLOGY FOR PLANT BREEDER'S RIGHTS

HortScience ◽  
1996 ◽  
Vol 31 (3) ◽  
pp. 324d-324
Author(s):  
Victor Luk ◽  
John Carlson
Keyword(s):  
Dna Fingerprinting ◽  
Ethidium Bromide ◽  
Genomic Dna ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Genome Structure ◽  
Cesium Chloride ◽  
Botanical Garden ◽  
Juvenile Form ◽  
Rapd Primer

DNA fingerprinting is a potentially powerful molecular genetic technique that can be used to distinguish subtle differences in genome structure among closely related genotypes, such as many horticultural varieties. A DNA fingerprinting project is currently in progress at the Univ. of British Columbia (UBC) Biotechnology Laboratory to produce a set of DNA markers and an easy, reliable, and legally recognized analysis protocol that will enable the UBC Botanical Garden Plant Introduction Scheme (PISBG) to unambiguously identify any of their released varieties, even in dormant or juvenile form, wherever it is being propagated or sold. High-quality genomic DNA was isolated from the leaf samples of six PISBG species (Anagallis monellii, Artemesia stelleriana, Clematis, Genista pilosa, Microbiota decussata, and Penstemon fruticosa) using a modified CTAB DNA isolation protocol, and further purified by cesium chloride/ethidium bromide gradient. Samples of these genomic DNA preparations (10 ng) were then amplified by a 45-cycle polymerase chain reaction (PCR) protocol using 1.5-μm 10-nucleotide primers of arbitrary nucleotide sequence that amplify a variety of sites distributed across the genome. Following the amplification, PCR products [random amplified polymorphic DNA (RAPD) markers] were separated by agarose gel electrophoresis and visualized by ethidium bromide staining. More than 70% of the 51 primers tested so far generated distinctive banding patterns (2–11 bands) with DNA samples from each species. Subtle changes in the genome or differences between genotypes can be detected by screening a series of such primers against DNA samples from the genotypes in question. Once a RAPD primer has been identified that consistently generates a different banding pattern between genotypes, it can be used as an identification tool for discriminating between those genotypes at any time in the future.

scholarly journals Comment on "AFLP data and the origins of domesticated crops"

Genome ◽  
2004 ◽  
Vol 47 (3) ◽  
pp. 615-620 ◽  
Author(s):  
F Salamini ◽  
M Heun ◽  
A Brandolini ◽  
H Özkan ◽  
J Wunder
Keyword(s):  
Phylogenetic Tree ◽  
Dna Fingerprinting ◽  
Phylogenetic Analyses ◽  
Tetraploid Wheat ◽  
Aflp Markers ◽  
Crop Domestication ◽  
Einkorn Wheat ◽  
Aflp Data ◽  
Fertile Crescent ◽  
Tree Topologies

We review some concepts and methods of handling and using DNA fingerprinting in phylogenetic analyses related to crop domestication. Particular reference is made to AFLP markers and mode and place of einkorn, barley, and tetraploid wheat domestication in the Neolithic by human communities in the Fertile Crescent. The reconsideration of AFLP databases of domesticated and wild lines demonstrates that phylogenetic tree topologies, originally described for the three species, match closely the new results obtained by principle coordinate analyse.Key words: AFLPs, discontinuous markers, crop domestication, einkorn wheat, barley, tetraploid wheat.

DNA fingerprinting analysis ofPetromyces alliaceus(AspergillussectionFlavi)

2005 ◽  
Vol 51 (12) ◽  
pp. 1039-1044 ◽  
Author(s):  
Cesaria E McAlpin ◽  
Donald T Wicklow
Keyword(s):  
Dna Fingerprinting ◽  
Aspergillus Flavus ◽  
Genomic Dna ◽  
Genotypic Diversity ◽  
Dna Probe ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Close Relationship ◽  
Aspergillus Alliaceus ◽  
Aspergillus Section

The objective of this study was to evaluate the ability of the Aspergillus flavus pAF28 DNA probe to produce DNA fingerprints for distinguishing among genotypes of Petromyces alliaceus (Aspergillus section Flavi), a fungus considered responsible for the ochratoxin A contamination that is occasionally observed in California fig orchards. P. alliaceus (14 isolates), Petromyces albertensis (one isolate), and seven species of Aspergillus section Circumdati (14 isolates) were analyzed by DNA fingerprinting using a repetitive sequence DNA probe pAF28 derived from A. flavus. The presence of hybridization bands with the DNA probe and with the P. alliaceus or P. albertensis genomic DNA indicates a close relationship between A. flavus and P. alliaceus. Twelve distinct DNA fingerprint groups or genotypes were identified among the 15 isolates of Petromyces. Conspecificity of P. alliaceus and P. albertensis is suggested based on DNA fingerprints. Species belonging to Aspergillus section Circumdati hybridized only slightly at the 7.0-kb region with the repetitive DNA probe, unlike the highly polymorphic hybridization patterns obtained from P. alliaceus and A. flavus, suggesting very little homology of the probe to Aspergillus section Circum dati genomic DNA. The pAF28 DNA probe offers a tool for typing and monitoring specific P. alliaceus clonal populations and for estimating the genotypic diversity of P. alliaceus in orchards, vineyards, or crop fields.Key words: Aspergillus alliaceus, Circumdati, DNA probe, genotypic diversity, hybridization patterns, ochratoxin, Southern blot.

scholarly journals Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum

1970 ◽  
Vol 7 ◽  
pp. 27-36 ◽  
Author(s):  
BK Joshi ◽  
K Okuno ◽  
R Ohsawa ◽  
T Hara
Keyword(s):  
Dna Fingerprinting ◽  
Genetic Relationship ◽  
Related Species ◽  
High Intensity ◽  
Genomic Dna ◽  
Single Band ◽  
Common Buckwheat ◽  
Research Journal ◽  
The Cost

If the EST primers designed for one species can be used in related species, then the cost involvedin developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. forother species is significantly reduced. We tested the applicability of 17 EST primers developedfrom common buckwheat in other wild and cultivated Fagopyrum species. A total of 18accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA cloneswere used to design primers using Primer3. Amplification products were different in bandintensity. In most of the cases, the bands of F. homotropicum were with high intensity. Allprimers showed single band except in Accession C9022. Three primers 23, 31 and 69 producedvery clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eightprimers amplified the DNA of all accessions. Results indicated that the transferability of ESTmarkers developed for common buckwheat decreased with an increase in genetic distancebetween them.Key words: Common buckwheat; EST markers; Fagopyrum species; transferabilityDOI: 10.3126/narj.v7i0.1863Nepal Agriculture Research Journal Vol.7 2006 pp.27-36

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