scholarly journals Oral immunoadjuvant activity ofLactobacillus caseisubsp.caseiin dextran-fed layer chickens

2006 ◽  
Vol 95 (2) ◽  
pp. 430-434 ◽  
Author(s):  
Tomohiko Ogawa ◽  
Yasuyuki Asai ◽  
Hiromi Sakamoto ◽  
Kenji Yasuda
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Specific Substrate ◽  
Infectious Bronchitis ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Actual Application ◽  
Colony Forming Units ◽  
Inactivated Vaccines ◽  
Layer Chickens

We recently reported that synbioticLactobacillus caseisubsp.caseitogether with specific substrate dextran elicited an enhancement in humoral immune response against bovine serum albumin (BSA) as a model antigen in BALB/c mice. The present study was designed to evaluate the oral immunoadjuvant effects of the synbiotic in layer chickens. Using a PCR assay,L. caseisubsp.caseiwas detected specifically in the intestinal chyme of chickens (10d of age, Julia strain) fedad libitumon a diet supplemented with 75mg dextran/kg (dextran-supplemented diet, DSD) and administered orally with 107colony-forming units (CFU)L. caseisubsp.caseiin 0·1ml PBS with the aid of an intubation needle at 1, 2 and 3d of age. Furthermore, oral administration of 107CFUL. caseisubsp.caseiat 1–3d of age significantly enhanced the production of anti-BSA antibody in DSD-fed chickens (60d of age) administered orally with 1mg BSA at 32 and 33d of age and subcutaneously with 5μg BSA at 33d of age. In addition, among bacterial numbers tested, 106CFUL. caseisubsp.caseitogether with dextran induced an effective increase in humoral immune response to mixed inactivated vaccines against Newcastle disease and avian infectious bronchitis, and the treatment may be advantageous in protecting against these infectious diseases in chickens in actual application. These results suggest that dietary supplementation ofL. caseisubsp.caseiwith dextran leads to immunomodulation of humoral immune responses.

scholarly journals Differences in systemic humoral immune response among Balb/c mice administered with probiotic, LPS Escherichia coli, and probiotic-LPS E. coli

2019 ◽  
Author(s):  
Kurniawan Taufiq Kadafi ◽  
Satrio Wibowo
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Humoral Immune Response ◽  
Control Group ◽  
E Coli ◽  
Humoral Immune ◽  
Treatment Regimens ◽  
Humoral Immune Responses ◽  
Group 4 ◽  
Group 2

Background and Objectives: The aim of this study was to compare the systemic humoral immune responses, including IgE, IgA, IgG and IgM levels in Balb/c mice administered a probiotic, LPS derived from Escherichia coli (E.coli), and probiot- ic-LPS derived from E. coli. Materials and Methods: Thirty-two male Balb/c mice, 10-12 weeks of age with body weight ranging from 30-40 g were randomly divided into four experimental groups (n=8). The treatment regimens were as follows: Group 1, mice did not receive LPS or probiotic (control group); Group 2, mice received only LPS on the first day; Group 3, mice received probi- otic for 7 days; Group 4, mice received LPS on the first day, and then continued, with probiotic for 7 days. The mice were observed for 8 days, and then, euthanized the next day (day 9). The serum was collected, and the levels of IgE, IgA, IgG and IgM were measured using ELISA. Results: The humoral immune response was higher in the presence of a probiotic compared to that in the control; IgE (9.02 ± 0.58 units/ml, p=0.000), IgA (3.26 ± 0.99 units/ml, p=0.316), IgG (7.29 ± 0.24 units/ml, p=0.000), and IgM (4.01 ± 2.98 units/ml, p=0.505). When administered with LPS E. coli along with probiotic, the humoral immune response was the highest; IgE (10.68 ± 1.63 units/ml, p=0.000), IgA (8.34 ± 1.47 units/ml, p=0.000), IgG (9.96 ± 0.98 units/ml, p=0.000), and IgM (4.31 ± 1.05 units/ml, p=0.319) compared to the control group. Conclusion: Probiotic-LPS derived from E. coli treatment induced a higher humoral immune response (highest IgE, IgA, IgG and IgM levels) compared to treatment with probiotic only.

Humoral immune responses induced by Gymnorhynchus gigas extracts in BALB/c mice

1999 ◽  
Vol 73 (3) ◽  
pp. 239-243 ◽  
Author(s):  
M. Rodero ◽  
C. Cuéllar
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Humoral Immune Response ◽  
Type I ◽  
Igg Antibody ◽  
Th2 Response ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Antibody Class ◽  
Allergic Disorders

The aim of this study was to determine if the plerocercoid larvae of Gymnorhynchus gigas, a common cestode of the ray’s bream (Brama raii), possess antigenic compounds potentially capable of provoking anaphylactic episodes. A murine experimental model, using BALB/c mice, was developed to study the humoral immune response induced by G. gigas extracts. A highly specific humoral immune response was detected and cross-reactions were not observed between parasite and host antigens. The presence of IgM and IgG3 levels suggest the presence of thymus-independent antigens in the parasitic extract. The IgG antibody class showed the highest levels, with the IgG1 the predominant subclass. These IgG1 levels are in accordance with the supposed presence of a type I allergic reaction after the ingestion of G. gigas plerocercoids parasitizing fish, as well as inducing anaphylaxia in fish. These results indicate that somatic products released from ingested larvae of G. gigas could induce the development of a Th2 response capable of causing allergic disorders.

scholarly journals Novel protein targets of the humoral immune response to Listeria monocytogenes infection in rabbits

2007 ◽  
Vol 56 (7) ◽  
pp. 888-895 ◽  
Author(s):  
Wei Ling Yu ◽  
Hanhong Dan ◽  
Min Lin
Keyword(s):  
Immune Response ◽  
Listeria Monocytogenes ◽  
Immune Responses ◽  
Humoral Immune Response ◽  
Protective Immunity ◽  
Rabbit Model ◽  
Protein Targets ◽  
Humoral Immune ◽  
Humoral Immune Responses

The role of the humoral immune response in protective immunity against listerial infection has been overlooked and is essentially unknown. This study aimed to discover the protein targets of Listeria monocytogenes that elicit an antibody response following infection in a rabbit model. A genomic expression library for L. monocytogenes was constructed and differentially screened to identify genes encoding proteins that reacted with antiserum from rabbits infected with live L. monocytogenes serotype 4b (RαL), but not with that from animals immunized with heat-killed bacteria (RαK). Thirty-one clones expressing proteins that reacted exclusively with RαL were identified and sequenced. Sequence analysis, together with Western blot analysis of the proteins expressed from positive clones, led to the identification of eight L. monocytogenes proteins as targets of humoral immune responses during listerial infection: three internalin members (InlA, InlD and InlC2) and five novel proteins of unknown function (designated IspA, IspB, IspC, IspD and IspE, respectively). Exhibition of humoral immune responses to these proteins in actively infected rabbits but not in animals receiving heat-killed L. monocytogenes suggested that they were induced or significantly upregulated in vivo during infection and thus are important in Listeria pathogenesis. With the exception of antibodies to InlA, this is the first demonstration of antibodies to the other seven proteins in infected hosts. These immunogenic proteins may be useful candidates for elucidation of the role of antibodies in protective immunity in the context of listerial infection, as well as potential targets for serodiagnostic reagents and vaccine and drug development.

scholarly journals Humoral Immune Response after Primary Rubella Virus Infection and after Vaccination

2007 ◽  
Vol 14 (5) ◽  
pp. 644-647 ◽  
Author(s):  
C. Vauloup-Fellous ◽  
L. Grangeot-Keros
Keyword(s):  
Immune Response ◽  
Virus Infection ◽  
Immune Responses ◽  
Humoral Immune Response ◽  
Rubella Virus ◽  
Primary Infection ◽  
Serum Samples ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Rubella Virus Infection

ABSTRACT We measured rubella virus immunoglobulin G (IgG) and IgM levels, as well as IgG avidity indexes, in serum samples taken before or after 6 months either after infection or after vaccination. The results obtained indicate that humoral immune responses are different after primary infection and after vaccination. This may have important consequences on the serological diagnosis of rubella virus infection.

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