scholarly journals Effects of photoperiod and feeding level on adipose tissue and muscle lipoprotein lipase activity and mRNA level in dry non-pregnant sheep

2001 ◽  
Vol 85 (3) ◽  
pp. 299-306 ◽  
Author(s):  
Y. Faulconnier ◽  
M. Bonnet ◽  
F. Bocquier ◽  
C. Leroux ◽  
Y. Chilliard
Keyword(s):  
Cardiac Muscle ◽  
Lipoprotein Lipase ◽  
Fatty Acid Synthase ◽  
Mrna Level ◽  
Energy Requirements ◽  
Mrna Levels ◽  
Feeding Level ◽  
Pregnant Sheep ◽  
Cardiac Muscles ◽  
Glycerol 3 Phosphate Dehydrogenase

The aim of the present study was to investigate the effects of photoperiod and feeding level on lipid metabolism in ovine perirenal and subcutaneous adipose tissues (AT) and in skeletal and cardiac muscles. Twenty dry non-pregnant ovariectomised ewes were divided into two groups and subjected to either 8 h or 16 h light/d, and underfed at 22 % energy requirements for 7 d. Half of the ewes in each group were slaughtered and the remaining ewes were refed at 190 % energy requirements for 14 d, until slaughtering. Refeeding increased (2.6–4.3-fold) malic enzyme (ME), fatty acid synthase (FAS), glucose-6-phosphate dehydrogenase (G6PDH) and glycerol-3-phosphate dehydrogenase (G3PDH) activities in subcutaneous AT as well as lipoprotein lipase (LPL) activity in perirenal (3.5-fold) and subcutaneous (10-fold) AT and to a lesser extent (1.4-fold) in the skeletal longissimus thoracis and cardiac muscles. Moreover, variations of LPL mRNA level followed variations of LPL activity: refeeding increased perirenal AT- and cardiac muscle-mRNA levels (7.4- and 2-fold respectively). The main finding of this study is that, for a given level of food intake, long days (compared with short days) increased the LPL activity in the longissimus thoracis muscle and, in refed ewes, the activities of LPL and ME in subcutaneous AT. Furthermore, long days increased LPL mRNA level in cardiac muscle and perirenal AT. Thus, our results show that there are direct effects of photoperiod on sheep AT lipogenic potential, as well as on muscle LPL activity, which are not caused by changes in nutrient availability.

scholarly journals Nutritional status induces divergent variations of GLUT4 protein content, but not lipoprotein lipase activity, between adipose tissues and muscles in adult cattle

2004 ◽  
Vol 92 (4) ◽  
pp. 617-625 ◽  
Author(s):  
Muriel Bonnet ◽  
Yannick Faulconnier ◽  
Jean-François Hocquette ◽  
François Bocquier ◽  
Christine Leroux ◽  
...  
Keyword(s):  
Cardiac Muscle ◽  
Lipoprotein Lipase ◽  
Fatty Acid Synthase ◽  
De Novo ◽  
Mrna Levels ◽  
Adult Cattle ◽  
Nutritional Conditions ◽  
Ruminant Animals ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Post Transcriptional Regulation

Metabolic adaptations to variations in food supply are incompletely understood in ruminant animal adipose tissue (AT) and muscle. To explore this, we studied lipid metabolism and glucose transport potential in one internal and one external AT, as well as in one oxidative and one glycolytic muscle from control, 7 d underfed and 21 d refed adult cows. Refeeding increased (+79 to +307 %) the activities of enzymes involved in de novo lipogenesis (fatty acid synthase, malic enzyme, glucose-6-phosphate dehydrogenase) in perirenal and subcutaneous AT; underfeeding did not modify these variables. Underfeeding decreased the activities of lipoprotein lipase (LPL) in perirenal AT (−70 %) and cardiac muscle (−67 %), but did not modify the activities in subcutaneous AT and longissimus thoracis. Refeeding increased LPL activities in all tissues (+40 to +553 %) to levels comparable with (cardiac muscle) or greater than (AT, longissimus thoracis) those observed in control cows. Such variations in perirenal and cardiac muscle LPL activities did not result from variations in LPL mRNA levels, but suggest a post-transcriptional regulation of LPL in these nutritional conditions. Underfeeding did not modify GLUT4 contents in perirenal AT and muscles, while refeeding increased it only in perirenal AT (+250 %). Our present results contrast with previous results in rats, where LPL is regulated in opposite directions in AT and muscles, and GLUT4 is generally increased by fasting and decreased by refeeding in skeletal muscles. The present results highlight the bovine specificity of the response, which probably arises in part from peculiarities of ruminant animals for nutrient digestion and absorption.

Fatty-acid synthase activity and mRNA level in hypertrophic type II cells from silica-treated rats

1994 ◽  
Vol 267 (2) ◽  
pp. L128-L136
Author(s):  
J. Rami ◽  
W. Stenzel ◽  
S. M. Sasic ◽  
C. Puel-M'Rini ◽  
J. P. Besombes ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Mrna Level ◽  
Type Ii Cells ◽  
Mrna Levels ◽  
Type Ii ◽  
Maximum Increase

Silica instillation causes a massive increase in lung surfactant. Two populations of type II pneumocytes can be isolated from rats administered silica by intratracheal injection: type IIA cells similar to type II cells from normal rats and type IIB cells, which are larger and contain elevated levels of surfactant protein A and phospholipid. Activities of choline-phosphate cytidylyltransferase, a rate-regulatory enzyme in phosphatidylcholine biosynthesis, and fatty-acid synthase (FAS) are increased in type IIB cells isolated from rats 14 days after silica injection. In the present study, we examined the increase in FAS and cytidylyltransferase activities in type IIB cells as a function of time after silica administration. FAS activity increased rapidly, was approximately threefold elevated 1 day after silica administration and has reached close to the maximum increase by 3 days. Cytidylyltransferase activity was not increased on day 1, was significantly increased on day 3 but was not maximally increased until day 7. Inhibition of de novo fatty-acid biosynthesis, by in vivo injection of hydroxycitric acid and inclusion of agaric acid in the type II cell culture medium, abolished the increase in cytidylyltransferase activity on day 3 but not FAS and had no effect on activities of two other enzymes of phospholipid synthesis. FAS mRNA levels were not increased in type IIB cells isolated 1-14 days after silica injection. These data show that the increase in FAS activity in type IIB cells is an early response to silica, that it mediates the increase in cytidylyltransferase activity, and that it is not due to enhanced FAS gene expression.

scholarly journals Adipose cell differentiation: evidence for a two-step process in the polyamine-dependent Ob1754 clonal line

1986 ◽  
Vol 238 (1) ◽  
pp. 115-122 ◽  
Author(s):  
E Z Amri ◽  
C Dani ◽  
A Doglio ◽  
J Etienne ◽  
P Grimaldi ◽  
...  
Keyword(s):  
Lipoprotein Lipase ◽  
Mrna Level ◽  
Growth Arrest ◽  
Clonal Line ◽  
Adipose Cell ◽  
Step Process ◽  
Late Emergence ◽  
Adipose Conversion ◽  
Early Expression ◽  
Glycerol 3 Phosphate Dehydrogenase

A subclone of preadipocyte Ob17 cells has been isolated (Ob1754 clonal line). Confluent Ob1754 cells treated with an inhibitor of spermidine and spermine synthesis, methylglyoxal bis(guanylhydrazone), were totally dependent upon putrescine addition for the expression of glycerol-3-phosphate dehydrogenase which behaved as a late marker of adipose conversion. Under these conditions, the early expression of lipoprotein lipase during growth arrest remained unchanged. Studies at the mRNA level showed that the expression of unidentified pOb24 and pGH3 mRNAs, which was parallel to that of lipoprotein lipase, is independent of polyamine addition whereas the late emergence of glycerol-3-phosphate dehydrogenase mRNA was putrescine-dependent and co-ordinated with the expression of pAL422 mRNA encoding for a myelin-P2 homologue [Bernlohr, Angus, Lane, Bolanowski & Kelly (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 5468-5472]. The appearance of lipoprotein lipase preceded DNA synthesis and post-confluent mitoses which were both putrescine-dependent and which took place before the appearance of glycerol-3-phosphate dehydrogenase. Thus the adipose conversion of Ob1754 cells involves the expression of at least two separate sets of markers which are differently regulated.

Effect of insulin on transcription of lipogenic and lipolytic-related genes and lipid metabolism in primary porcine adipocytes

2006 ◽  
Vol 3 (2) ◽  
pp. 135-140
Author(s):  
Lu Jian-Xiong ◽  
Chen Fen-Fen ◽  
Yang Gong-She
Keyword(s):  
Fatty Acid Synthase ◽  
Lipolytic Activity ◽  
Binding Protein ◽  
Regulatory Element ◽  
Mrna Level ◽  
Hormone Sensitive Lipase ◽  
Mrna Levels ◽  
Tissue Samples ◽  
Element Binding Protein ◽  
High Insulin

AbstractPrimary adipocytes from subcutaneous adipose tissue samples obtained from 7-day-old Yorkshire×Landrace crossbreed piglets were exposed to 0–400 nmol/l of insulin for 48 h. The accumulated triglyceride was measured through Oil Red O staining and the cumulative glycerol released was determined to assess lipolytic activity in adipocytes. Transcription levels of sterol regulatory element binding protein (SREBP)-1c, carbohydrate response element binding protein (ChREBP), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), and hormone-sensitive lipase (HSL) were assessed using reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that ChREBP and ACC1 mRNA levels were not influenced by insulin alone under low glucose (5 nmol/l). FAS mRNA level was markedly stimulated by all doses of insulin except 200 nmol/l, and SREBP-1c mRNA level increased with 100–300 nmol/l insulin. High insulin doses (300 and 400 nmol/l) increased the HSL mRNA level as well as lipolytic activity.

In vivo regulation of SREBP-1c in skeletal muscle: effects of nutritional status, glucose, insulin, and leptin

2004 ◽  
Vol 287 (1) ◽  
pp. R218-R227 ◽  
Author(s):  
S. Renee Commerford ◽  
Li Peng ◽  
John J. Dubé ◽  
Robert M. O'Doherty
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Nutritional Status ◽  
Fatty Acid Synthase ◽  
Gastrocnemius Muscle ◽  
Uncoupling Protein ◽  
Regulatory Element ◽  
Mrna Level ◽  
Mrna Levels ◽  
Fas Mrna

Sterol regulatory element binding protein-1c (SREBP-1c), a transcription factor that is important for mediating insulin effects on metabolic gene expression in liver during the fasted-to-fed transition, is also expressed in skeletal muscle. However, the regulation and role of SREBP-1c in skeletal muscle are poorly understood. The present study compared the effects of nutritional status, physiological hyperinsulinemic clamps, and adenovirus-mediated hyperleptinemia (HLEP) in rats on expression of SREBP-1c and other metabolic genes in skeletal muscle. Three- and 6-h refeeding of 18-h-fasted animals increased levels of SREBP-1c mRNA and the SREBP-1 protein (full length and mature) in gastrocnemius muscle ( P < 0.05). Fatty acid synthase (FAS) and hexokinase II (HKII) mRNA levels were also increased by refeeding, and uncoupling protein 3 (UCP3) mRNA level was decreased (all P < 0.05). Surprisingly, 3-h hyperinsulinemic clamps did not increase gastrocnemius muscle SREBP-1c and FAS mRNA levels or SREBP-1 protein levels but did increase HKII mRNA levels and decrease UCP3 mRNA levels ( P < 0.05). HLEP reduced refeeding-induced increases of SREBP-1c and FAS mRNA levels but did not reduce the level of SREBP-1 protein. We conclude that 1) skeletal muscle SREBP-1c gene expression is regulated by nutritional status in a fashion similar to that observed in liver and adipose tissue, 2) physiological hyperinsulinemia is not sufficient to imitate the effects of refeeding on SREBP-1c gene expression, and 3) leptin suppresses refeeding effects on SREBP-1c mRNA levels.

scholarly journals Dietary acetic acid reduces serum cholesterol and triacylglycerols in rats fed a cholesterol-rich diet

2006 ◽  
Vol 95 (5) ◽  
pp. 916-924 ◽  
Author(s):  
Takashi Fushimi ◽  
Kazuhito Suruga ◽  
Yoshifumi Oshima ◽  
Momoko Fukiharu ◽  
Yoshinori Tsukamoto ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Bile Acid ◽  
Total Cholesterol ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Mrna Level ◽  
Serum Total Cholesterol ◽  
Free Access ◽  
Mrna Levels ◽  
Atp Citrate Lyase

To investigate the efficacy of the intake of vinegar for prevention of hyperlipidaemia, we examined the effect of dietary acetic acid, the main component of vinegar, on serum lipid values in rats fed a diet containing 1% (w/w) cholesterol. Animals were allowed free access to a diet containing no cholesterol, a diet containing 1% cholesterol without acetic acid, or a diet containing 1% cholesterol with 0·3% (w/w) acetic acid for 19 d. Then, they were killed after food deprivation for 7 h. Cholesterol feeding increased serum total cholesterol and triacylglycerol levels. Compared with the cholesterol-fed group, the cholesterol and acetic acid-fed group had significantly lower values for serum total cholesterol and triacylglycerols, liver ATP citrate lyase (ATP-CL) activity, and liver 3-hydroxy-3-methylglutaryl-CoA content as well as liver mRNA levels of sterol regulatory element binding protein-1, ATP-CL and fatty acid synthase (P<,0·05). Further, the serum secretin level, liver acyl-CoA oxidase expression, and faecal bile acid content were significantly higher in the cholesterol and acetic acid-fed group than in the cholesterol-fed group (P<0·05). However, acetic acid feeding affected neither the mRNA level nor activity of cholesterol 7a-hydroxylase. In conclusion, dietary acetic acid reduced serum total cholesterol and triacylglycerol: first due to the inhibition of lipogenesis in liver; second due to the increment in faecal bile acid excretion in rats fed a diet containing cholesterol.

scholarly journals (-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

2017 ◽  
Vol 43 (2) ◽  
pp. 812-831 ◽  
Author(s):  
Longlong Li ◽  
Mengling Peng ◽  
Chongyang Ge ◽  
Lei Yu ◽  
Haitian Ma
Keyword(s):  
Energy Metabolism ◽  
Lipid Droplets ◽  
Fatty Acid Synthase ◽  
Mrna Level ◽  
Mrna Levels ◽  
Biochemical Mechanism ◽  
Atp Citrate Lyase ◽  
Acetyl Coa ◽  
Hydroxycitric Acid ◽  
Citrate Lyase

Background/Aims: (-)-Hydroxycitric acid (HCA) had been shown to suppress fat accumulation in animals and humans, while the underlying biochemical mechanism is not fully understood, especially little information is available on whether (-)-HCA regulates energy metabolism and consequently affects fat deposition. Methods: Hepatocytes were cultured for 24 h and then exposed to (-)-HCA (0, 1, 10, 50 µM), enzyme protein content was determined by ELISA; lipid metabolism gene mRNA levels were detected by RT-PCR. Results: (-)-HCA significantly decreased the number and total area of lipid droplets. ATP-citrate lyase, fatty acid synthase and sterol regulatory element binding protein-1c mRNA level were significantly decreased after (-)-HCA treatment, whereas peroxisome proliferator-activated receptor α mRNA level was significantly increased. (-)-HCA significantly decreased ATP-citrate lyase activity and acetyl-CoA content in cytosol, but significantly increased glucose consumption and mitochondrial oxygen consumption rate. (-)-HCA promoted the activity/content of glucokinase, phosphofructokinase-1, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, aconitase, succinate dehydrogenase, malate dehydrogenase, NADH dehydrogenase and ATP synthase remarkably. Conclusions: (-)-HCA decreased lipid droplets accumulation by reducing acetyl-CoA supply, which mainly achieved via inhibition of ATP-citrate lyase, and accelerating energy metabolism in chicken hepatocytes. These results proposed a biochemical mechanism of fat reduction by (-)-HCA in broiler chickens in term of energy metabolism.

scholarly journals A Switch in Dehydrogenase to Reductase Activity of 11β-Hydroxysteroid Dehydrogenase Type 1 upon Differentiation of Human Omental Adipose Stromal Cells

2002 ◽  
Vol 87 (3) ◽  
pp. 1205-1210 ◽  
Author(s):  
Iwona J. Bujalska ◽  
Elizabeth A. Walker ◽  
Martin Hewison ◽  
Paul M. Stewart
Keyword(s):  
Dehydrogenase Activity ◽  
Lipoprotein Lipase ◽  
Stromal Cells ◽  
Adipocyte Differentiation ◽  
Reductase Activity ◽  
Hydroxysteroid Dehydrogenase ◽  
Mrna Levels ◽  
Adipose Stromal Cells ◽  
Glycerol 3 Phosphate Dehydrogenase

As exemplified in patients with Cushing’s syndrome, glucocorticoids play an important role in regulating adipose tissue distribution and function, but circulating cortisol concentrations are normal in most patients with obesity. However, human omental adipose stromal cells (ASCs) can generate glucocorticoid locally through the expression of the enzyme 11β-hydroxysteroid dehydrogenase (11β-HSD) type 1 (11β-HSD1), which, in intact cells, has been considered to be an oxoreductase, converting inactive cortisone (E) to cortisol (F). Locally produced F can induce ASC differentiation, but the relationship between 11β-HSD1 expression and adipocyte differentiation is unknown. Primary cultures of paired omental (om) and sc ASC and adipocytes were prepared from 17 patients undergoing elective abdominal surgery and cultured for up to 14 d. Expression and activity of 11β-HSD isozymes were analyzed together with early (lipoprotein lipase) and terminal (glycerol 3 phosphate dehydrogenase) markers of adipocyte differentiation. On d 1 of culture, 11β-HSD1 activity in intact om ASCs exceeded oxoreductase activity in every patient (78.9 ± 24.9 vs. 15.8 ± 3.7 [mean ± se] pmol/mg per hour, P &lt; 0.001), and in sc ASCs, relative activities were similar (40.6 ± 12.2 vs. 36.9 ± 8.8). Conversely, in freshly isolated om adipocytes, reductase activity exceeded dehydrogenase activity (23.6 ± 1.5 vs. 6.2 ± 0.8 pmol/mg per hour, P &lt; 0.01). Following 14 d of culture in serum-free conditions with addition of 10 nm insulin (Ctr) or insulin with 100 nm F (+F), lipoprotein lipase/18S RNA levels increased in both the Ctr- and +F-treated ASCs, but glycerol 3 phosphate dehydrogenase increased only in the +F cultures. In both cases, however, 11β-HSD1 oxoreductase activity exceeded dehydrogenase activity (Ctr: 53.3 ± 9.0 vs. 32.4 ± 10.5, P &lt; 0.05; +F: 65.6 ± 15.6 vs. 37.1 ± 11.5 pmol/mg per hour, P &lt; 0.05), despite no significant changes in 11β-HSD1 mRNA levels. In sc ASCs, dehydrogenase activity was similar to reductase activity in both Ctr- and +F-treated cells. Type 2 11β-HSD expression was undetectable in each case. These data show that in intact, undifferentiated om ASCs, 11β-HSD1 acts primarily as a dehydrogenase, but in mature adipocytes oxoreductase activity predominates. Because glucocorticoids inhibit cell proliferation, we postulate that 11β-HSD1 activity in uncommitted ASCs may facilitate proliferation rather than differentiation. Once early differentiation is initiated, a “switch” to 11β-HSD1 oxoreductase activity generates F, thus promoting adipogenesis. Site-specific regulation of the set-point of 11β-HSD1 activity may be an important mechanism underpinning visceral obesity.

Regulation of expression of the lipoprotein lipase gene in brown adipose tissue

1992 ◽  
Vol 263 (3) ◽  
pp. E500-E506 ◽  
Author(s):  
J. R. Mitchell ◽  
A. Jacobsson ◽  
T. G. Kirchgessner ◽  
M. C. Schotz ◽  
B. Cannon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Mrna Level ◽  
Mrna Levels ◽  
Lipoprotein Lipase Activity ◽  
Lipase Gene ◽  
Lipoprotein Lipase Gene ◽  
Brown Adipose

The regulation of lipoprotein lipase gene expression in brown adipose tissue was studied. Rats were preacclimated to 21 degrees C. Exposure to cold (4 degrees C) resulted in a rapid increase in the level of lipoprotein lipase mRNA in the tissue. The level peaked (expressed per microgram total RNA) after approximately 8 h and then slowly declined. The increased lipoprotein lipase mRNA level was not due to an increased stability of the mRNA, but, in a transition event from a high to a low expression of the lipoprotein lipase gene, a transcription-dependent process was recruited that accelerated the breakdown of lipoprotein lipase mRNA. Norepinephrine injections increased lipoprotein lipase mRNA levels in the tissue; this effect was mediated via a beta-adrenergic receptor. The effect of cold could be mimicked by norepinephrine injections, and these two effects were not additive, indicating that the cold effect was mediated by norepinephrine. The lipoprotein lipase mRNA level was also increased by insulin injections (into fasted animals); thus an increase in lipoprotein lipase gene expression in brown adipose tissue may be induced via two different stimuli, which, intracellularly, would be mediated via different signaling systems. In all investigated conditions, the changes in lipoprotein lipase mRNA levels observed here were parallelled by alterations in lipoprotein lipase activity reported earlier from this laboratory. It was therefore concluded that, under the conditions studied, lipoprotein lipase activity in brown adipose tissue was primarily regulated at the transcriptional level.

Lipid-binding proteins and lipoprotein lipase activity in human skeletal muscle: influence of physical activity and gender

2004 ◽  
Vol 97 (4) ◽  
pp. 1209-1218 ◽  
Author(s):  
Bente Kiens ◽  
Carsten Roepstorff ◽  
Jan F. C. Glatz ◽  
Arend Bonen ◽  
Peter Schjerling ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Lipoprotein Lipase ◽  
Binding Proteins ◽  
Mrna Level ◽  
Lipid Binding ◽  
Mrna Levels ◽  
Training Status ◽  
Fatty Acid Binding ◽  
Muscle Lipid ◽  
Lipid Binding Proteins

The protein and mRNA levels of several muscle lipid-binding proteins and the activity and mRNA level of muscle lipoprotein lipase (mLPL) were investigated in healthy, nonobese, nontrained (NT), moderately trained, and endurance-trained (ET) women and men. FAT/CD36 protein level was 49% higher ( P < 0.05) in women than in men, irrespective of training status, whereas FAT/CD36 mRNA was only higher ( P < 0.05) in women than in men in NT subjects (85%). Plasma membrane-bound fatty acid binding protein (FABPpm) content was higher in ET men compared with all other groups, whereas training status did not affect FABPpm content in women. FABPpm mRNA was higher ( P < 0.05) in NT women than in ET women and NT men. mLPL activity was not different between gender, but mLPL mRNA was 160% higher ( P < 0.001) in women than in men. mLPL activity was 48% higher ( P < 0.05) in ET than in NT subjects, irrespective of gender, in accordance with 49% higher ( P < 0.05) mLPL mRNA in ET than in NT subjects. A 90-min exercise bout induced an increase ( P < 0.05) in FAT/CD36 mRNA (∼25%) and FABPpm mRNA (∼15%) levels in all groups. The present study demonstrated that, in the NT state, women had higher muscle mRNA levels of several proteins related to muscle lipid metabolism compared with men. In the ET state, only the gender difference in mLPL mRNA persisted. FAT/CD36 protein in muscle was higher in women than in men, irrespective of training status. These findings may help explain gender differences in lipid metabolism and, furthermore, suggest that the balance between gene transcription, translation, and possibly breakdown of several proteins in muscle lipid metabolism depend on gender.

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