scholarly journals Effect of 3 months vitamin E supplementation on indices of the cellular and humoral immune response in elderly subjects

1997 ◽  
Vol 78 (5) ◽  
pp. 761-774 ◽  
Author(s):  
Frouwkje G. De Waart ◽  
Lützen Portengen ◽  
Gert Doekes ◽  
Corné J. Verwaal ◽  
Frans J. Kok
Keyword(s):  
Vitamin E ◽  
Mononuclear Cells ◽  
Tocopheryl Acetate ◽  
Elderly Subjects ◽  
Control Group ◽  
Antioxidant Vitamin ◽  
Immune Responsiveness ◽  
Humoral Immune ◽  
Cellular Immune Responsiveness ◽  
Cellular Immune

It has been suggested that decreased immune responsiveness in the elderly may be counteracted by the antioxidant vitamin E. In a 3-month double-blind placebo-controlled intervention trial among elderly subjects aged 65 years and over we studied the effects of a daily dose of 100 mg dl-α-tocopheryl acetate on the cellular immune responsiveness (n 52) measured by the in vitro response of peripheral blood mononuclear cells (PBMC) to the mitogens concanavalin A (ConA) and phytohaemagglutinin (PHA). Also effects on the humoral immune responsiveness (n 74) were investigated by measuring immunoglobulin (Ig)G, IgG4 and IgA antibody concentrations against various common antigens. In the vitamin E group plasma α-tocopherol increased by 51 % (P = 0.0001) during intervention whereas no significant changes were observed in the control group. Initial proliferative PBMC responses differed between the vitamin E group and the control group whereas all other baseline characteristics were comparable. No significant changes were observed in cellular immune responsiveness when adjusted for initial values in either the control group or the vitamin E group and, after the trial period, responses in the two groups were not significantly different. Similarly, in the vitamin E group no significant changes were found in levels of IgG and IgA raised against Penicillium or IgG4 raised against egg, milk, or wheat proteins. In the control group small but significant increases in IgG anti-Penicillium (P <0.05) and decreases in IgG4 against milk proteins (P < 0.05) were observed. Thus, the results of this study performed with the relatively low dose of 100 mg dl-α-tocopheryl acetate do not support the claims of a beneficial effect of vitamin E intake on the overall immune responsiveness of elderly subjects.

scholarly journals Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Reza Taherkhani ◽  
Fatemeh Farshadpour ◽  
Manoochehr Makvandi ◽  
Hamid Rajabi Memari ◽  
Ali Reza Samarbafzadeh ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Immune Responses ◽  
Mononuclear Cells ◽  
Hepatitis E ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Orf2 Protein ◽  
Cellular Immune ◽  
Iranian Patients

Background.The aim of this study was to evaluatehepatitis E virus(HEV) specific cellular immune responses to truncated ORF2 protein in Iranian patients recovered from HEV infection. Information about HEV-specific immune responses could be useful in finding an effective way for development of HEV vaccine.Methods.A truncated form of HEV ORF2 protein containing amino acids 112-608 was used to stimulate peripheral blood mononuclear cells (PBMCs) separated from HEV-recovered and control groups. Finally, the levels of four cytokines, IFN-γELISPOT, and cell proliferative responses following stimulation with the truncated ORF2 protein were assessed in the both groups.Results.The truncated ORF2 protein was able to induce IFN-γELISPOT and cell proliferation responses and to produce significant amounts of IFN-γand IL-12 cytokines, but low amounts of IL-10 and IL-4 cytokinesin vitro. These responses were significantly higher in the recovered group compared to the control group. These results indicate the antigenic nature of the truncated ORF2 protein and production of T helper type 1 cytokines.Conclusion.The truncated ORF2 protein can effectively induce significant cellular immune responsesand can be introduced as a potential vaccine candidate. However, further studies are required to evaluate this proteinin vivo.

Cellular Immune Responsiveness of Uveitis Patients to Retinal S-Antigen

1980 ◽  
Vol 89 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Robert B. Nussenblatt ◽  
Igal Gery ◽  
Elmer J. Ballintine ◽  
Waldon B. Wacker
Keyword(s):  
Immune Responsiveness ◽  
Cellular Immune Responsiveness ◽  
Cellular Immune

Vitamin E Does Not Reduce Cardiomyopathy In Doxorubicin treated Rats

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4944-4944
Author(s):  
Jhon A Guerra ◽  
Maribel Torres-Serrant ◽  
Pedro J Santiago
Keyword(s):  
Vitamin E ◽  
Conflicts Of Interest ◽  
Cardiac Injury ◽  
Free Radical Scavenger ◽  
Alpha Tocopherol ◽  
Control Group ◽  
Antioxidant Vitamin ◽  
Radical Scavenger ◽  
Doxorubicin Treatment ◽  
Doxorubicin Group

Abstract Abstract 4944 Introduction: The clinical efficacy of doxorubicin is severely limited by its cardiotoxicity. Antioxidants represent the largest class of chemicals examined as potential protective agents and for which there is continuing interest. Dexrazoxane is the current agent used for the control of doxorubicin related cardiotoxicity. However, in large trials the incidence was reduced only by 50% (1). Vitamin E, a known antioxidant and free radical scavenger agent, has been evaluated in the past as a cardio protective drug in animal models receiving doxorubicin but contradictory conclusions regarding this effect have been reported (2, 3). We hypothesized whether Vitamin E has an effect in cardioprotection in rats receiving doxorubicin. Design and Methods: Three groups of 6 Rats each were used for the experiment. Group 1 received doxorubicin 1.5mg/kg intraperitoneally (IP) weekly for 9 weeks for a total cumulative dose of 13.5mg/kg. Group 2 received Vitamin E 100 IU/kg/weekly by IP injection, 48 hours prior to the doxorubicin. Group 3 received 0.5 cc of saline solution 0.9% IP weekly for 9 weeks as control group. Functional parameters including plasmatic nitric oxide (NO) levels and cardiac ejection fraction (EF) were determined on each group at the end of the experiment. Results: Doxorubicin treatment significantly increased plasmatic NO concentration when compared with controls (35.30 ± 5.63 mM vs. 14.72 ± 2.66 mM, n=6, P=0.016); however, in the group of rats receiving Vitamin E prior to doxorubicin, NO did not have a significant decrease (from 35.30 ± 5.63 mM to 31.77± 8.91 mM n=6, P=0.75). Regarding EF, doxorubicin treatment decreased EF significantly when compared with saline controls rats (59 ± 5.61% vs. 77 ± 3.89 %, n=6, P<0.05); however, in the group of rats receiving Vitamin E prior to doxorubicin, EF did not have a significant improvement (from 59 ± 5.61% to 69.17 ± 4.4, n=6, P=0.24). Conclusion: These results suggest that Vitamin E does not prevent or reduce cardiac injury in rats treated with doxorubicin. Different alternatives including other antioxidants could be explored in an attempt to decrease cardiotoxicity produced by doxorubicin and to improve the effect of the standard cardioprotective agent used Dexrazoxane. Further studies are necessary. References: 1. Swain SM. Adult multicenter trials using dexrazoxane to protect against cardiac toxicity. Semin Onco 1998; 25 (4 Suppl 10):43-7 2. Breed JG, Zimmerman AN, Dormans JA, Pinedo HM. Failure of the antioxidant vitamin E to protect against adriamycin-induced cardiotoxicity in the rabbit. Cancer Res 1980; 40:2033-8 3. Myers CE, McGuire W, Young R. Adriamycin: amelioration of toxicity by alpha-tocopherol. Cancer Treat Rep 1976; 60:961-2 Disclosures: No relevant conflicts of interest to declare.

Using phytohaemagglutinin to determine immune responsiveness in saltwater crocodiles (Crocodylus porosus)

2013 ◽  
Vol 61 (4) ◽  
pp. 301 ◽  
Author(s):  
John W. Finger Jr ◽  
Amanda L. Adams ◽  
Peter C. Thomson ◽  
Cathy M. Shilton ◽  
Greg P. Brown ◽  
...  
Keyword(s):  
Agricultural Production ◽  
Immune Status ◽  
Acquired Immunity ◽  
Immune Responsiveness ◽  
Trade Offs ◽  
Crocodylus Porosus ◽  
Cellular Immune Responsiveness ◽  
Antigenic Exposure ◽  
Cellular Immune ◽  
Phosphate Buffered Saline

Immune responsiveness, the ability of an organism to effectively respond immunologically following antigenic exposure, is an essential component of life history, as organisms require effective immune functionality in order to grow, survive and reproduce. However, immune status is also associated with concomitant trade-offs in these physiological functions. Herein we demonstrate the validation of phytohaemagglutinin (PHA) injection in saltwater crocodiles, Crocodylus porosus, to assess cellular immune responsiveness. Following injection of 2 mg mL–1 PHA into the hind toe webbing, we observed a peak swelling response 12 h after injection, with PHA inducing increased thickness compared with webs injected with phosphate-buffered saline (PBS) (F5,518 = 145.13, P < 0.001). Subsequent injections increased responsiveness relative to the primary injection response (F5,290 = 2.92, P = 0.029), suggesting that PHA exposure induced immunological memory, a tenet of acquired immunity. Histological examination revealed that PHA-injected toe webs displayed increased numbers of leukocytes (granulocytes, macrophages, and lymphocytes) relative to PBS-injected webs, with peak leukocytic infiltrate observed 12 h after injection. We suggest the use of PHA injection in crocodilians as a measure of cellular immune responsiveness in agricultural (production and animal welfare), ecological, and toxicological contexts.

CELLULAR IMMUNE RESPONSIVENESS IN CIGARETTE SMOKERS

The Lancet ◽  
1974 ◽  
Vol 303 (7868) ◽  
pp. 1232-1233 ◽  
Author(s):  
R.H. Whitehead ◽  
BeverleyE. Hooper ◽  
D.A. Grimshaw ◽  
L.E. Hughes
Keyword(s):  
Immune Responsiveness ◽  
Cigarette Smokers ◽  
Cellular Immune Responsiveness ◽  
Cellular Immune

scholarly journals Vitamin E and Selenium Decreased Cortisol in Cattle

2020 ◽  
Vol 64 (4) ◽  
pp. 49-54
Author(s):  
P. Mudroň
Keyword(s):  
Vitamin E ◽  
Abdominal Surgery ◽  
Dairy Cows ◽  
Serum Vitamin ◽  
Selenium Concentration ◽  
Serum Cortisol ◽  
Average Concentration ◽  
Tocopheryl Acetate ◽  
Control Group ◽  
Stress Changes

AbstractTwenty dairy cows with left abomasal displacement were used to investigate the effects of vitamin E and selenium treatment on blood cortisol in dairy cows stressed by omentopexy. The cows were randomly divided into two groups. Ten hours before surgery 6 g of DL-α-tocopheryl acetate (6 mg.kg−1) and 67 mg of natrium selenite (0.1 mg.kg−1) in a volume of 40 ml were administered subcutaneously to 10 cows; the control animals (n = 10) received an equivalent volume of injectable water (40 ml). The injection of vitamin E and selenium produced a rapid rise (P < 0.05) in blood α-tocopherol and selenium concentrations. The serum vitamin E increased several times ten hours after vitamin E and Se injection and raised continuously to the highest average concentration of 21.6 mg.l−1 at hour 24 after the surgery. The highest selenium concentration was seen ten hours after selenium administration with holding the increased concentrations in comparison to initial ones during the whole study. Serum cortisol increased in both groups after surgery. The highest cortisol concentrations were reached at one hour after surgery in the experimental and control group (56.7 ± 28.8 and 65.3 ± 26.1 μg.l−1, respectively). A return to the levels similar to the initial ones was recognized 24 hours after the surgery. The ANOVA revealed a significant effect of vitamin E and selenium injection on serum cortisol (P < 0.05). In conclusion, we have demonstrated that abdominal surgery resulted in typical stress changes with a weaker cortisol response to the abdominal surgery in animals treated with vitamin E and selenium.

scholarly journals α-Tocopherol in breast milk of women with preterm delivery after a single postpartum oral dose of vitamin E

2016 ◽  
Vol 115 (8) ◽  
pp. 1424-1430 ◽  
Author(s):  
Jeane Franco Pires Medeiros ◽  
Karla Danielly da Silva Ribeiro ◽  
Mayara Santa Rosa Lima ◽  
Renata Alexandra Moreira das Neves ◽  
Amanda Cibely Pinheiro Lima ◽  
...  
Keyword(s):  
Vitamin E ◽  
Breast Milk ◽  
Controlled Trial ◽  
Intervention Group ◽  
Relevant Information ◽  
Maternal Serum ◽  
Tocopheryl Acetate ◽  
Control Group ◽  
Mature Milk ◽  
Tocopherol Concentration

AbstractWe evaluated the effect of maternal vitamin E supplementation on the α-tocopherol concentrations of colostrum, transitional milk and mature milk of women who had given birth prematurely. This longitudinal randomised-controlled trial divided eighty-nine women into two groups: a control group and a supplemented group. Blood and breast milk were collected from all the participants after delivery. Next, each woman in the supplemented group received 400 IU of RRR-α-tocopheryl acetate. Further breast milk samples were collected 24 h after the first collection, as well as 7 and 30 d after delivery. α-Tocopherol concentrations were determined by HPLC. The baseline α-tocopherol concentrations in the maternal serum of the two groups were similar: 1159·8 (sd 292·4) μg/dl (27·0 (SD 6·8) μmol/l) for the control group and 1128·3 (sd 407·2) μg/dl (26·2 (SD 9·5) μmol/l) for the supplemented group. None of the women was vitamin E deficient. Breast milk α-tocopherol concentrations increased by 60 % 24 h after supplementation in the intervention group and did not increase at all in the control group. α-Tocopherol concentration of the transitional milk in the supplemented group was 35 % higher compared with the control group. α-Tocopherol concentrations of the mature milk in both groups were similar. Maternal supplementation with 400 IU of RRR-α-tocopherol increased the vitamin E concentrations of the colostrum and transitional milk, but not of the mature milk. This study presents relevant information for the design of strategies to prevent and combat vitamin E deficiency in the risk group of preterm infants.

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