Dietary intake and micronutrient status of adolescents: effect of vitamin and trace element supplementation on indices of status and performance in tests of verbal and non-verbal intelligence

1994 ◽  
Vol 71 (6) ◽  
pp. 897-918 ◽  
Author(s):  
Susan Southon ◽  
A. J. A. Wright ◽  
P. M. Finglas ◽  
Angela L. Bailey ◽  
Julie M. Loughridge ◽  
...  
Keyword(s):  
Verbal Intelligence ◽  
Trace Mineral ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Food Composition Tables ◽  
Micronutrient Status ◽  
Dietary Record ◽  
And Performance ◽  
Biochemical Indices ◽  
Duplicate Diets

Relationships between micronutrient intake and status, and micronutrient status and performance in tests of intelligence were investigated in a group of adolescents (13–14 years old). Dietary intakes were assessed using a 7 d weighed dietary record method, coupled with the collection of duplicate diets. Vitamin and trace mineral intakes calculated using food composition tables were compared with those obtained by direct analysis of duplicate diets. Micronutrient status was judged via a range of biochemical indices measured in blood samples taken after a 12–15 h fast. Blood samples were taken both before and after a 16-week period of vitamin and trace mineral supplementation. Individual tests of verbal and nonverbal intelligence were also performed pre- and post-supplementation. The results of this study indicate that the use of food table data may lead to substantial over- or underestimation of the intake of several micronutrients. In general, the total calculated or analysed amount of a specific micronutrient consumed did not adequately predict status, as judged by a range of biochemical indices. There were significant changes in status measurements over the 16-week study period, irrespective of supplementation, and these changes were markedly influenced by the initial status of the subject. There was no effect of supplementation on performance in tests of intelligence. However, there was a significant association between plasma ascorbic acid and initial non-verbal intelligence quotient (IQ) in the boys, and between whole blood glutathione peroxidase (EC1.11.1.9) activity and non-verbal and verbal IQ in both sexes. These findings are discussed in relation to other recent studies of the influence of micronutrient supplementation on the psychological performance of children.

scholarly journals Vitamin C intake and plasma ascorbic acid concentration in adolescents

1993 ◽  
Vol 69 (2) ◽  
pp. 563-576 ◽  
Author(s):  
P. M. Finglas ◽  
Angela Bailey ◽  
A. Walker ◽  
Julie M. Loughridge ◽  
A. J. A. Wright ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Direct Analysis ◽  
Food Group ◽  
Dietary Intakes ◽  
Plasma Ascorbic Acid ◽  
Food Composition Tables ◽  
Dietary Record ◽  
The Relationship ◽  
Duplicate Diets

The relationship between vitamin C intake and status was investigated in a group of adolescents (13–14 years old). Dietary intakes were assessed using a 7 d weighed dietary record method, coupled with the collection of duplicate diets. Vitamin C intakes calculated using food composition tables were compared with values obtained by direct analysis of duplicate diets. Vitamin C status was judged via measurement of plasma ascorbic acid (AA) concentration in blood samples taken after a 12–15 h fast. The relationship between calculated and analysed vitamin C intake and plasma AA concentration was examined. Average daily calculated vitamin C intakes, for the group (n 54) as a whole over a 7 d period, gave a good estimate of intake, as judged by prompt analysis of duplicate diets. However, analysed v. calculated intakes were significantly different for approximately one-third of subjects when data were examined on an individual basis. Large discrepancies between analysed and calculated values could not be accounted for on a food group basis. In all but two individuals, calculated vitamin C intake was in excess of the new reference nutrient intake (RNI, part of the new daily reference values (Department of Health and Social Security, 1991)) of 40 mg and all plasma AA concentrations were well above those used to indicate even a moderate risk of deficiency. A relationship between vitamin C intake and plasma AA was observed for both males (n 19) and females (n 35). However, the relationship was much stronger for males who showed a wider range of both intake and plasma AA values.

Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months

2018 ◽  
Vol 88 (3-4) ◽  
pp. 151-157 ◽  
Author(s):  
Scott W. Leonard ◽  
Gerd Bobe ◽  
Maret G. Traber
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Frozen Storage ◽  
Blood Collection ◽  
Plasma Samples ◽  
Optimal Conditions ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Sample Handling

Abstract. To determine optimal conditions for blood collection during clinical trials, where sample handling logistics might preclude prompt separation of erythrocytes from plasma, healthy subjects (n=8, 6 M/2F) were recruited and non-fasting blood samples were collected into tubes containing different anticoagulants (ethylenediaminetetra-acetic acid (EDTA), Li-heparin or Na-heparin). We hypothesized that heparin, but not EDTA, would effectively protect plasma tocopherols, ascorbic acid, and vitamin E catabolites (α- and γ-CEHC) from oxidative damage. To test this hypothesis, one set of tubes was processed immediately and plasma samples were stored at −80°C, while the other set was stored at 4°C and processed the following morning (~30 hours) and analyzed, or the samples were analyzed after 6 months of storage. Plasma ascorbic acid, as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used. Neither time prior to processing, nor anticoagulant, had any significant effects upon plasma α- or γ-tocopherols or α- or γ-CEHC concentrations. α- and γ-tocopherol concentrations remained unchanged after 6 months of storage at −80°C, when measured using either LC-ECD or LC/mass spectrometry. Thus, refrigeration of whole blood at 4°C overnight does not change plasma α- or γ-tocopherol concentrations or their catabolites. Ascorbic acid is unstable in whole blood when EDTA is used as an anticoagulant, but when whole blood is collected with heparin, it can be stored overnight and subsequently processed.

Effect of trace mineral source on mineral status and performance of beef steers fed low- or high-sulfur diets

2017 ◽  
Vol 95 (9) ◽  
pp. 4139
Author(s):  
S. J. Hartman ◽  
O. N. Genther-Schroeder ◽  
S. L. Hansen
Keyword(s):  
Beef Steers ◽  
Trace Mineral ◽  
Mineral Status ◽  
And Performance

scholarly journals Influence of Pre-Sampling Handling Duration on Selected Biochemical Indices in the Common Pheasant (Phasianus colchicus)

2009 ◽  
Vol 78 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Petr Chloupek ◽  
Eva Voslářová ◽  
Pavel Suchý ◽  
Iveta Bedáňová ◽  
Vladimíra Pištěková ◽  
...  
Keyword(s):  
Czech Republic ◽  
Glucose Concentration ◽  
Plasma Concentrations ◽  
Plasma Corticosterone ◽  
Phasianus Colchicus ◽  
Blood Samples ◽  
Common Pheasant ◽  
The Common ◽  
Glucose Plasma ◽  
Biochemical Indices

The effects of varying periods of pre-sampling handling (1.5 min, 3 min, 4.5 min, 6 min) on selected biochemical indices were monitored in a group of 8–9-month-old common pheasants (Phasianus colchicus) kept at a pheasantry in Jinačovice, Czech Republic. The duration of pheasant handling (capture, restraint, and blood sampling) was positively correlated with plasma corticosterone (p < 0.001) and lactate (p < 0.05) levels. Negative correlations were found between the handling duration and glucose concentration (p < 0.01), and aspartate aminotransferase level (p < 0.05) in the blood plasma. A significant increase in corticosterone plasma concentrations and a decrease in glucose plasma concentrations were already found in blood samples taken after 3 min of capture in comparison to blood samples of pheasants taken within 1.5 min.

Abstract WP240: Ultra-acute Diagnostics for Stroke: Large-scale Implementation of Prehospital Biomarker Sampling

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Olli S Mattila ◽  
Heini Harve ◽  
Saana Pihlasviita ◽  
Juhani Ritvonen ◽  
Gerli Sibolt ◽  
...  
Keyword(s):  
Acute Stroke ◽  
Large Scale ◽  
Biomarker Discovery ◽  
Cost Effective ◽  
Blood Sampling ◽  
Blood Samples ◽  
And Performance ◽  
Comprehensive Stroke Center ◽  
Stroke Center ◽  
Acute Diagnostics

Background and purpose: Blood-based biomarkers could enable early and cost-effective diagnostics for acute stroke patients in the prehospital setting to support early initiation of treatments. However, large prehospital sample sets required for biomarker discovery and validation are missing, and the feasibility of large-scale blood sampling by emergency medical services (EMS) has not been determined. We set out to establish extensive prehospital blood sampling of thrombolysis candidates in the catchment area of our comprehensive stroke center, with a 1.5 million population base. Methods: EMS personnel were trained to collect prehospital blood samples using a cannula-adapter technique. Time delays, sample quality and performance bottlenecks were investigated between May 20, 2013 and May 19, 2014. Results: Prehospital blood sampling and study recruitment were successfully performed in 430 thrombolysis candidates, of which 55.3% were admitted outside office hours. The median (interquartile range) emergency call to prehospital sample time was 33 minutes (25-41), and the median time from reported symptom onset or wake-up to prehospital sample was 53 minutes (38-85; n=394). Prehospital sampling was performed 31 minutes (25-42) earlier than admission blood sampling, and 37 minutes (30-47) earlier than admission neuroimaging. Quality control data from 25 participating EMS units indicated a 4-minute increase in median transport time (from arrival on-scene to hospital door) for study patients compared to patients of the preceding year. The hemolysis rate in serum and plasma samples was 6.5% and 9.3% for EMS samples, and 0.7% and 1.6% for admission samples collected with venipuncture. Conclusions: Prehospital biomarker sampling is feasible in standard EMS units and provides a median timesaving of over 30 minutes to obtain first blood samples. Large biobanks of prehospital blood samples will facilitate development of ultra-acute stroke biomarkers.

scholarly journals Effects of maternal supplementation with an injectable trace mineral on subsequent calf performance and inflammatory response1

2019 ◽  
Vol 97 (11) ◽  
pp. 4475-4481 ◽  
Author(s):  
Rebecca S Stokes ◽  
Mareah J Volk ◽  
Frank Ireland ◽  
Daniel W Shike
Keyword(s):  
Body Temperature ◽  
Feed Efficiency ◽  
Average Daily Gain ◽  
Area Under The Curve ◽  
Serum Cortisol ◽  
Trace Mineral ◽  
Blood Samples ◽  
Lps Challenge ◽  
Calf Performance ◽  
Maternal Supplementation

Abstract Newly weaned, commercial Angus steers [body weight (BW) = 204 ± 19 kg; n = 24; 12 steers from dams administered an injectable trace mineral (MM; Mulimin90) and 12 steers from control (CON) dams] were utilized to determine the effects of maternal supplementation with an injectable trace mineral on the inflammatory response of subsequent steers subjected to a lipopolysaccharide (LPS) challenge at the initiation of a 42-d receiving period. On day −2 steers were weaned, and the following day, shipped 354 km to the Beef Cattle and Sheep Field Laboratory in Urbana, IL. On day 0, steers were administered an intravenous LPS challenge. Body temperature and blood samples were collected from steers prior to LPS administration (0 h) and again at 0.5, 1, 2, 3, 4, 5, and 6 h. Blood samples were analyzed for trace mineral and cortisol at 0 and 2 h and glucose, insulin, LPS-binding protein (LBP), interleukin-1β (IL-1β), interleukin-6 (IL-6), haptoglobin, ceruloplasmin, and fibrinogen at 0, 0.5, 1, 2, 3, 4, 5, and 6 h. Calf BW was collected at trial initiation and subsequently every 14 d. Dry matter intake was collected daily and average daily gain (ADG) and feed efficiency were assessed. Initial plasma Zn tended (P = 0.06) to be greater for MM steers. However, there was no difference (P ≥ 0.31) in trace mineral status or serum cortisol at any other time. Total area under the curve (TAUC) for body temperature was lesser (P > 0.01) for MM steers. Basal LBP concentrations and TAUC for LBP tended (P ≤ 0.10) to be greater for MM steers. Peak concentration of IL-6 tended (P = 0.09) to be reached earlier for CON steers. However, there was no difference (P ≥ 0.11) in glucose, insulin, IL-6, ceruloplasmin, haptoglobin, and fibrinogen concentrations between treatments. Calf performance and feed efficiency did not differ (P ≥ 0.17) between treatments except ADG from day 28 to 42, which was greater (P = 0.03) for CON steers. Maternal supplementation with an injectable trace mineral tended to improve steer plasma Zn status at 0 h and tended to increase basal concentrations of LBP and overall LBP production when steers were administered an LPS challenge. Additionally, MM steers exhibited a more favorable change in body temperature following LPS administration. However, injectable trace mineral supplementation of dams during gestation had minimal to no effect on cytokine and acute-phase protein concentrations, as well as overall calf performance and efficiency during a 42-d receiving period.

Effects of 4- and 8-h preexercise feedings on substrate use and performance

1989 ◽  
Vol 67 (5) ◽  
pp. 2066-2071 ◽  
Author(s):  
M. G. Flynn ◽  
T. J. Michaud ◽  
J. Rodriguez-Zayas ◽  
C. P. Lambert ◽  
J. B. Boone ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Maximal Oxygen Uptake ◽  
Work Output ◽  
Total Work ◽  
Cycling Exercise ◽  
Blood Samples ◽  
Substrate Use ◽  
Feeding Trials ◽  
The Subject ◽  
And Performance

Seven well-trained male cyclists were studied during 105 min of cycling (65% of maximal oxygen uptake) and a 15-min “performance ride” to compare the effects of 4- and 8-h preexercise carbohydrate (CHO) feedings on substrate use and performance. A high CHO meal was given 1) 4-h preexercise (M-4), 2) 8-h preexercise (M-8), 3) 4-h preexercise with CHO feedings during exercise (M-4CHO), and 4) 8-h preexercise with CHO feedings during exercise (M-8CHO). Blood samples were obtained at 0, 15, 60, 105, and 120 min and analyzed for lactate, glucose, insulin, and glycerol. Total work output during the performance ride was similar for the M-4 (217,893 +/- 13,348 N/m) and M-8 trials (216,542 +/- 13,905) and was somewhat higher for the M-4CHO (223,994 +/- 14,387) and M-8CHO (224,702 +/- 15,709) trials (P = 0.059, NS). Glucose was significantly elevated throughout exercise, and insulin levels were significantly elevated at 15 and 60 min during M-4CHO and M-8CHO compared with M-4 and M-8 trials. Glycerol levels were significantly lower during the CHO feeding trials compared with placebo and were not significantly different during exercise when the subject had fasted an additional 4 h. The results of this study suggest that when preexercise meals are ingested 4 or 8 h before submaximal cycling exercise, substrate use and performance are similar.

Effect of Active and Passive Recovery on Blood Lactate and Performance During Simulated Competition in High Level Gymnasts

2003 ◽  
Vol 28 (2) ◽  
pp. 240-256 ◽  
Author(s):  
Monèm Jemni ◽  
William A. Sands ◽  
Françoise Friemel ◽  
Paul Delamarche
Keyword(s):  
Blood Lactate ◽  
Sitting Position ◽  
Active Recovery ◽  
Blood Samples ◽  
Passive Recovery ◽  
Recovery Strategies ◽  
Lactate Removal ◽  
And Performance ◽  
Recovery Protocols ◽  
High Level

The purpose of this study was to investigate the effect of two recovery strategies between men's gymnastics events on blood lactate removal (BL) and performance as rated by expert "blind" judges. Twelve male gymnasts (21.8 ± 2.4 years) participated. The sessions were composed of routine performances in the six Olympic events, which were separated by 10 min of recovery. All gymnasts performed two recovery protocols between events on separate days: Rest protocol, 10 min rest in a sitting position; combined protocol, 5 min rest and 5 min self-selected active recovery. Three blood samples were taken at 2, 5, and 10 min following each event. Gymnasts produced moderate values of BL following each of the six events (2.2 to 11.6 mmolúL−1). There was moderate variability in BL values between events that could not be accounted for by the athlete's event performance. Gymnasts showed higher BL concentration (p > .05) and significantly (p < .05) higher scoring performances (as rated by a panel of certified judges) when they used a combined recovery between gymnastics events rather than a passive recovery (ΔBL = 40.51% vs. 28.76% of maximal BL, p < .05, and total score = 47.28 ± 6.82 vs. 38.39 ± 7.55, p < .05, respectively). Key words: oxidation, removal, heart rate

scholarly journals Perinatal viral hepatitis C and performance capabilities for its therapy

2014 ◽  
Vol 19 (3) ◽  
pp. 4-8
Author(s):  
L. G Goryacheva ◽  
N. V Rogozina ◽  
V. A Greshnyakova ◽  
V. V Ivanova
Keyword(s):  
Hepatitis C ◽  
Clinical Symptoms ◽  
Antiviral Drug ◽  
Sustained Viral Response ◽  
Hcv Infection ◽  
Control Group ◽  
Viral Hepatitis C ◽  
Significant Difference ◽  
And Performance ◽  
Biochemical Indices

The aim of the study: to establish the rate of the vertical transmission of HCV infection and justify the usefulness of the drug a-IFN2b (Viferon®) in children with perinatal infection with hepatitis C virus (HCV) at the early stages of the disease. There were examined 465 children aged 2.5 months to 3 years old (at the time of the enrollment in the study), born to mothers with HCV infection and the frequency of transmission from mother to child was established to be is 7.1% (33 cases). In 54.5% the genotype 1b was recorded, in 3% - 1a, and in 6.0 % - 2 genotype. Viferon® drug in doses of 3х10 6IU/m 2 was introduced to 17 infants aged from 3 to 12 months (duration of the course: 6 months in cases with 3, 2 genotype and 12 months - in patients with genotype 1). The efficacy of therapy was 58.8 % (RNA HCV (-) 10 cases. In prescription the drug to children under 6 months the efficacy was 62.5%, under 6-12 months - 44.4%. In the control group (16patients) spontaneous viral clearance was established to be in 18.7% of cases. The medication did not cause adverse effects and was well tolerated by children. In none out of the 17 children treated with the drug Viferon®, we have not reported adverse events (clinical symptoms, abnormalities in the hemogram and biochemical indices). The obtained data confirm the expediency of the early use of antiviral drug Viferon® in the treatment of children with perinatal HCV. Nо significant difference in the efficacy of therapy in different genotypes of the virus was identified. Virtually in all children (9 out of 10 children) responded to Viferon® therapy, there was noted Sustained Viral Response

scholarly journals 92 Health and Performance of Ewes in Woodland and Silvopasture Systems

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 16-16
Author(s):  
Nevershi Ellis ◽  
Uma Karki ◽  
Bidur Paneru ◽  
Lila B Karki
Keyword(s):  
Body Condition Score ◽  
Small Ruminants ◽  
Live Weight ◽  
Pinus Palustris ◽  
Animal Performance ◽  
Blood Samples ◽  
Ewe Lambs ◽  
Fecal Egg Count ◽  
And Performance ◽  
Grazing Lands

Abstract The health and performance of small ruminants can be influenced by the type of vegetation available in the grazing lands, which are evolving from conventional sole pastures to other systems containing browse species, such as pastures incorporated with browse, silvopastures, and woodlands. However, how the type of grazing lands would impact animal performance and health is not documented well. The objective of this study was to determine the health and performance of ewe lambs in southern-pine silvopastures and woodlands. The study site consisted of pine silvopastures (3 plots, 0.4-ha each) planted with different cool- and warm-season forages, and woodlands (3 plots, 0.4-ha each) having numerous understory shrubs and herbaceous species. Katahdin-St. Croix-cross ewe lambs (n = 20; live weight – 29.4±1.03 kg., 5–6 months old) were rotationally stocked in study plots (10 animals/system) from May to August 2019. Both silvopasture and woodland systems consisted of 14-year-old longleaf (Pinus palustris Mill.) and loblolly pine (Pinus taeda L.) trees. Animal performance data [live weight (LW), body condition score (BCS), FAMACHA score, and fecal egg count] were collected every 14 days and blood samples collected at the beginning and on day 80 after they were stocked in the study plots. Blood samples were analyzed for PVC using centrifuge machine and other parameters in IDEXX. Data were analyzed using the multivariate analysis of variance in SAS 9.4. The silvopasture ewes gained more weight versus the woodland ewes (P &lt; 0.0001). Fecal egg count was correlated negatively with live weight and BCS and positively with FAMACHA score in both groups (P &lt; 0.05). Woodland ewes showed greater levels of RBC, hemoglobin, and lymphocyte, but less blood urea nitrogen (BUN), monocyte, and basophil than silvopasture ewes (P &lt; 0.05). Results indicate that woodland ewes may need some supplement to improve their weight gain and BUN.

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