scholarly journals The digestibility in piglets of faba bean (Vicia faba L.) as affected by breeding towards the absence of condensed tannins

1992 ◽  
Vol 68 (3) ◽  
pp. 793-800 ◽  
Author(s):  
A. F. B. Van Der Poel ◽  
L. M. W. Dellaert ◽  
A. Van Norel ◽  
J. P. F. G. Helsper
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Trypsin Inhibitor Activity ◽  
Positive Effects ◽  
Faba Beans

Seed samples from two near-isogenic faba bean (Vicia faba L.) lines were examined for the levels of so-called anti-nutritional factors (ANF). From the ANF known to be present in faba beans, trypsin inhibitor activity, functional lectins, condensed tannins and pyrimidine glycosides were analysed. It was concluded that the lines differed only in the content of condensed tannins being < 0.5 g/kg and 5.2 (sd 0.2) g/kg for the low (LT)- and high (HT)-tannin lines respectively. In addition, the level of pyrimidine glycosides in the LT line was slightly higher than that in the HT line. The LT line showed a reduced proportion of the seed coat (105 v. 119 g/kg) and a lower seed weight (0.85 v. 1.01 g). The apparent ileal and faecal digestibility values of dry matter and nitrogen from the HT and LT line were determined for piglets which were fed on diets containing chromium oxide as a marker. The mean apparent ileal and faecal digestibility values for dry matter for the LT line were 0.694 and 0.889 and for N 0.828 and 0.879 respectively. For the HT line, these values were approximately 0.05 and 0.10 lower (P > 0.05). A multi-enzyme technique was used to predict the in vitro protein digestibility (IVPD) of the two lines. The IVPD of the LT line was 0.965 and about 0.05 higher than the HT line, confirming the difference in digestibility as measured in vivo. The present study shows the positive effects on digestibility of removal of condensed tannins in faba beans which was achieved by plant breeding.

FACTORS AFFECTING IN VITRO RUMEN DIGESTION OF FABA BEAN CULTIVARS (Vicia faba L.)

1983 ◽  
Vol 63 (1) ◽  
pp. 89-96 ◽  
Author(s):  
K. E. BUCKLEY ◽  
T. J. DEVLIN ◽  
R. R. MARQUARDT
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Protein Digestibility ◽  
In Vitro Digestibility ◽  
Protein Concentrate ◽  
Dry Matter Digestibility ◽  
Vicia Faba L

Experiments were conducted investigating the in vitro dry matter digestibility of eight faba bean (Vicia faba L. var. Major and Vicia faba L. var. Minor) cultivars. The testa of the different cultivars had a tannin content of 0−6.4%. The effect of adding condensed tannins on in vitro dry matter digestibility and in vitro protein digestibility of faba bean protein concentrate was also examined. Whole bean in vitro dry matter digestibility was greater (P < 0.05) in tannin-free faba bean cultivars than in those containing tannin. When the cotyledons and testa of some of the cultivars were examined separately, this difference was shown to be largely due to the higher digestibility of testa from the tannin-free cultivar. Regression analysis indicated that the in vitro digestibility of the whole bean was related to both the tannin and lignin contents of the testa. Autoclaving various faba bean fractions decreased (P < 0.05) the in vitro dry matter digestibility of the protein concentrate, whole bean and cotyledons, but had no effect on the digestibility of testa and starch. Adding condensed tannins, isolated from faba bean testa (cv. Diana), to the in vitro fermentation media decreased (P < 0.05) the in vitro dry matter and protein digestibility of the faba bean concentrate. The added tannins may have exerted their effect by binding the substrate protein. Key words: In vitro digestion, faba bean cultivars

scholarly journals Are Faba Bean and Pea Proteins Potential Whey Protein Substitutes in Infant Formulas? An In Vitro Dynamic Digestion Approach

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 362
Author(s):  
Linda Le Roux ◽  
Olivia Ménard ◽  
Raphaël Chacon ◽  
Didier Dupont ◽  
Romain Jeantet ◽  
...  
Keyword(s):  
Faba Bean ◽  
Whey Proteins ◽  
Milk Proteins ◽  
Protein Digestibility ◽  
Cow Milk ◽  
Partial Substitution ◽  
Infant Formulas ◽  
Pea Proteins

Infant formulas (IFs) are used as substitutes for human milk and are mostly based on cow milk proteins. For sustainability reasons, animal protein alternatives in food are increasingly being considered, as plant proteins offer interesting nutritional and functional benefits for the development of innovative IFs. This study aimed to assess how a partial substitution (50%) of dairy proteins with faba bean and pea proteins influenced the digestibility of IFs under simulated dynamic in vitro digestion, which were set up to mimic infant digestion. Pea- and faba bean-based IFs (PIF and FIF, respectively) have led to a faster aggregation than the reference milk-based IF (RIF) in the gastric compartment; that did not affect the digesta microstructure at the end of digestion. The extent of proteolysis was estimated via the hydrolysis degree, which was the highest for FIF (73%) and the lowest for RIF (50%). Finally, it was apparent that in vitro protein digestibility and protein digestibility-corrected amino acid score (PDCAAS)-like scores were similar for RIF and FIF (90% digestibility; 75% PDCAAS), but lower for PIF (75%; 67%). Therefore, this study confirms that faba bean proteins could be a good candidate for partial substitution of whey proteins in IFs from a nutritional point of view, provided that these in vitro results are confirmed in vivo.

Quantitative genetic studies of yield, yield components, and phenological and agronomic characters in spring faba bean

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 169-173 ◽  
Author(s):  
H. M. Kao ◽  
P. B. E. McVetty
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Yield Components ◽  
Harvest Index ◽  
Dry Matter ◽  
Diallel Analysis ◽  
Diallel Cross ◽  
Agronomic Characters ◽  
Vicia Faba L ◽  
Faba Beans

Hayman's diallel cross analysis was employed to investigate the nature of the genetic control and heritability of yield, yield components, and phenological and agronomic characters in F1 and F2 generations of spring faba beans (Vicia faba L.). High-yielding S4 inbred lines from five open-pollinated faba bean cultivars were used as parents to generate complete F1 and F2 diallels. The S5 inbred line parents and the 20 cross combinations were planted in randomized complete block experiments with six replications. All characters in the F1 diallel and in the F2 diallel with the exception of days from planting to maturity met all of the assumptions required for Hayman's diallel analysis. Yield, total dry matter, harvest index, and pods per plant exhibited significant apparent overdominance in both the F1 and F2 diallels. It is concluded that substantial immediate increases in yield and total dry matter could be expected from exploiting the apparent overdominant gene action found for these characters in these crosses via F1 hybrids or synthetics. Key words: total dry matter, harvest index, diallel crosses, inheritance, Vicia faba L.

Effect of condensed tannins in Lotus corniculatus on the nutritive value of pasture for sheep

1997 ◽  
Vol 128 (3) ◽  
pp. 365-372 ◽  
Author(s):  
G. C. WAGHORN ◽  
I. D. SHELTON
Keyword(s):  
Soluble Protein ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Nutritive Value ◽  
Experimental Period ◽  
Lotus Corniculatus ◽  
Feeding Trial ◽  
Wool Growth

The extent to which condensed tannins (CT) in Lotus corniculatus were able to affect protein degradation and the nutritive value of pasture were evaluated at Palmerston North, New Zealand in 1992 in an indoor feeding trial with sheep and using in vitro incubations. The feeding trial involved three groups of seven young wether sheep held indoors in metabolism crates for the 32-day experimental period with one group fed freshly cut ryegrass/clover pasture (Pasture group) whilst the other two groups received a mixture of c. 37% freshly cut Lotus corniculatus and 63% pasture (dry matter (DM) basis). One of the latter groups was given twice daily oral drenches of polyethylene glycol (PEG) to bind with and remove the effects of the CT (PEG group) whilst the remaining sheep (Tannin group) were not drenched. Intakes of sheep given the mixed diets were constrained to that of the pasture group. The CT concentration in the lotus was 26·1 g/kg DM so that the mixed diets contained c. 10g CT/kg DM. Effects of CT on digestion and sheep performance were minimal. Apparent digestibility of N was lower in the Tannin group (76·4%) than PEG (79·8%) or Pasture (79·4%) sheep (P<0·001), but there were no differences in DM digestibility (75%) or wool growth from mid-side patches. Rumen ammonia and soluble protein concentrations appeared to be similar in PEG and Tannin sheep and volatile fatty acid (VFA) concentrations were similar in all groups (100 mmol/l).For incubations in vitro, several ratios of Lotus corniculatus: pasture were used to determine the effects of CT on precipitation of soluble proteins and the net yield of ammonia and gas with and without PEG. Homogenates of Lotus corniculatus leaves and pasture showed that 50% of soluble protein was precipitated by 1·3% CT in homogenate DM and there was a substantial decrease in in vitro degradation to ammonia when lotus leaf accounted for 33% or more of DM with pasture. This work emphasises the need for caution when extrapolating from in vitro to the in vivo situation and suggests that 1% CT from Lotus corniculatus may be insufficient to affect the nutritive value of fresh forages for sheep.

Effect of sainfoin on in vitro digestion of fresh alfalfa and bloat in steers

1999 ◽  
Vol 79 (2) ◽  
pp. 203-212 ◽  
Author(s):  
L. R. McMahon ◽  
W. Majak ◽  
T. A. McAllister ◽  
J. W. Hall ◽  
G. A. Jones ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Condensed Tannins ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Dry Matter Intake ◽  
Full Bloom ◽  
Onobrychis Viciifolia ◽  
Ad Libitum

The effects of sainfoin (Onobrychis viciifolia) on digestion of alfalfa (Medicago sativa) were investigated in vitro and in vivo. Fresh alfalfa and sainfoin were incubated in an artificial rumen (Rusitec) in ratios of 100:0, 75:25, 50:50, 25:75 and 0:100 (as-fed). Disappearances of dry matter and N from sainfoin were 77 and 65% of those from alfalfa, respectively. Protease and endoglucanase activities, NH3-N and methane production declined (P < 0.05) as sainfoin increased. Bacterial numbers and microbial outputs were unchanged (P > 0.05), but cells incorporated more 15NH3N as sainfoin in the diet increased. Chopped leaves (100:0, 95:5 and 90:10 alfalfa:sainfoin) were incubated for 48 h with diluted ruminal fluid containing 0 or 50 mg polyethylene glycol, which binds tannins. Gas and volatile fatty acid productions were similar (P > 0.05) across treatments, but including 10% sainfoin (without polyethylene glycol) reduced (P < 0.05) NH3 concentrations between 8 and 24 h. Sainfoin tannins reduced degradation of forage protein without affecting the digestibility of the nonprotein fraction. Alfalfa herbage was fed alone or with early- to full-bloom sainfoin herbage (at 10 or 20% of ad libitum alfalfa dry matter intake) or with sainfoin hay or pellets, to eight Jersey steers in crossover trials conducted over 4 yr. Including sainfoin in the diet reduced (P < 0.001) the incidence of bloat by 45 to 93% in 3 of 4 yr, irrespective of the form in which it was supplied. Co-feeding sainfoin can markedly reduce the incidence of bloat in ruminants consuming fresh alfalfa. Key words: Alfalfa, sainfoin, bloat, condensed tannins

scholarly journals Chemical Profile and In Vitro Bioactivity of Vicia faba Beans and Pods

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 45
Author(s):  
Monica Rosa Loizzo ◽  
Marco Bonesi ◽  
Mariarosaria Leporini ◽  
Tiziana Falco ◽  
Vincenzo Sicari ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Radical Scavenging ◽  
Human Consumption ◽  
Potential Health ◽  
Antioxidant Power ◽  
Ic50 Value ◽  
Faba Beans ◽  
Alpha Glucosidase

Vicia faba L. (faba bean) (Fabaceae) is cultivated worldwide as a crop for human consumption. In this study, beans and pods were investigated for their phytochemical content and their potential nutraceutical properties as a strategy to counteract metabolic syndrome (MetS). Pods represent a faba bean industrial processing by-product. Pod ethanol extract showed the highest total phenol and flavonoid content. High Performance Liquid Chromatography (HPLC) analysis revealed that in both pods and bean, (+)-catechin and (−)-epicatechin were the two most abundant compounds. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), Ferric Reducing Antioxidant Power (FRAP), β-carotene bleaching were used to test V. faba antioxidant activity. The inhibition of alpha-amylase, alpha-glucosidase, and lipase was studied. Pod extract showed an ABTS radical scavenging ability (IC50 value of 1.5 mg/mL) comparable to ascorbic acid (IC50 value of 1.7 mg/mL) used as a positive control, whereas bean extract was the most active in protecting lipid peroxidation. A promising alpha-glucosidase inhibitory activity was also observed with the edible portion of faba beans (IC50 value of 38.31 mg/mL). Collectively, our results demonstrated the potential health properties of V. faba edible and inedible portions.

scholarly journals Methane production from in vitro incubation of kikuyu grass, lucerne and forages containing condensed tannins

2005 ◽  
pp. 147-153 ◽  
Author(s):  
M.H. Tavendale ◽  
L.P. Meagher ◽  
Z.A. Park-Ng ◽  
G.C. Waghorn ◽  
G.T. Attwood
Keyword(s):  
Fatty Acid ◽  
New Zealand ◽  
Methane Production ◽  
Condensed Tannins ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Methane Emissions ◽  
Kikuyu Grass

A series of in vitro incubations with kikuyu grass (Pennisetum clandestinum), lucerne and six legumes containing condensed tannins (CT) were undertaken to evaluate this technique against in vivo trials in New Zealand, measuring methane emissions. Published trials have demonstrated a reduction in methane emissions associated with CT and in one instance from kikuyu. The incubations used fresh minced forage (equivalent to 0.5 g dry matter (DM)) and were carried out in 50 ml sealed bottles containing buffer and rumen inoculum. Gas was sampled through a septum to monitor volume and composition throughout the 24h incubation. Incubation for 24 h resulted in 2.4-6.6 % conversion of DM to methane, and suggested CT concentrations below about 8% of the DM can reduce methane production without inhibiting fermentation rate. Higher concentrations of CT (> 8%) were associated with a lower rate of digestion. In common with in vivo trials, CT concentration in forage DM was inversely related to methane (adjusted R2 = 0.49; P = 0.01) and volatile fatty acid (adjusted R2=0.86; P

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