Erythrocytes, erythrocyte membranes, neutrophils and platelets as biopsy materials for the assessment of zinc status in humans

Manuel Ruz; Kelley R. Cavan; William J. Bettger; Rosalind S. Gibson

doi:10.1079/bjn19920109

Erythrocytes, erythrocyte membranes, neutrophils and platelets as biopsy materials for the assessment of zinc status in humans

British Journal Of Nutrition ◽

10.1079/bjn19920109 ◽

1992 ◽

Vol 68 (2) ◽

pp. 515-527 ◽

Cited By ~ 45

Author(s):

Manuel Ruz ◽

Kelley R. Cavan ◽

William J. Bettger ◽

Rosalind S. Gibson

Keyword(s):

Alkaline Phosphatase ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Erythrocyte Membranes ◽

Zn Deficiency ◽

Molar Ratios ◽

Potential Index ◽

Zinc Depletion ◽

Zn Status ◽

Cellular Immune

During a controlled zinc depletion-repletion study, fifteen men aged 25.3 (sd 3.3) years were fed on a low-Zn diet with high phytate:Zn and phytate × calcium: Zn molar ratios for 7 weeks, followed by a 2 week repletion period when 30 mg supplemental Zn/d was given. Changes in plasma, urine, and hair Zn concentrations, taste acuity, and cellular immune response confirmed the development of mild Zn deficiency. Zn concentrations in neutrophils, platelets, erythrocytes and erythrocyte membranes, mean platelet volume, and activities of alkaline phosphatse (EC3.1.3.1) and α-d-mannosidase (EC3.2.1.24) in neutrophils did not respond to changes in Zn status. In contrast, alkaline phosphatase activity in erythrocyte membranes showed a significant decline which was consistent in all subjects (nmol product formed/min per mg protein; baselinev.7-week Zn depletion, 0.656 (sd 0.279)v.0.506 (sd 0.230), at 7 weeks;P< 0.05); neutral phosphatase activity remained unchanged. Alkaline phosphatase activity in erythrocyte membranes may be a potential index of Zn status in humans

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Lipid deposition in intestine as a possible cause of malabsorption of nutrients in zinc-deficient common carp (Cyprinus carpio)

British Journal Of Nutrition ◽

10.1079/bjn19940077 ◽

1994 ◽

Vol 72 (5) ◽

pp. 753-762 ◽

Cited By ~ 1

Author(s):

S.K. Taneja ◽

P. Arya

Keyword(s):

Alkaline Phosphatase ◽

Glucose Uptake ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

The Other ◽

Zn Deficiency ◽

Lipid Deposition ◽

High Lipid ◽

Triacylglycerol Concentration ◽

High Lipid Diet

An experiment was performed to examine the interaction between Zn deficiency and lipid intake in carp. The carp were given a high-lipid diet that was either Zn-deficient (ZD) or Zn-supplemented (ZS), or were pair-fed (PF) the ZS diet to the intake of the ZD group. After 8 weeks the carp were killed and measurements were made of intestinal glucose uptake, levels of DNA, RNA and triacylglycerol, and alkaline phosphatase (EC3.1.3.1) activity in liver and intestine samples. A further group of similar carp were given the same diets but at week 8 were transferred to low-lipid diets, with the exception of half the ZD group. After a further 8 weeks of treatment, carps were killed for biochemical studies. Intestinal [14C]glucose uptake, levels of DNA, RNA and alkaline phosphatase activity in intestine and liver were significantly (P< 0·05) lower in the high-lipid ZD group than in the high-lipid ZS and PF diet groups. The triacylglycerol concentration in the intestine was higher in the high-lipid ZD group than in the other two groups. When the carp were given the corresponding low-lipid diets, the variables measured in intestine and liver of the ZD group were close to those of the other groups. The results of this study demonstrate that lipid, when present in excess in the diet, accumulates in the intestine under Zn-deficient conditions and may reduce the absorption of glucose in carp. The reduced RNA and DNA levels and alkaline phosphatase activity in liver and intestine of ZD fish compared with those of ZS fish given high- lipid diets is proposed to be due to the malabsorption of nutrients linked with lipid deposition in the intestine, rather than their dependence on the level of Zn in the diet.

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LEUCOCYTE METABOLISM IN PREGNANCY

Acta Endocrinologica ◽

10.1530/acta.0.xxxv0575 ◽

1960 ◽

Vol XXXV (IV) ◽

pp. 575-584 ◽

Cited By ~ 2

Author(s):

C. Borel ◽

J. Frei ◽

A. Vannotti

Keyword(s):

Alkaline Phosphatase ◽

Oxygen Consumption ◽

Pregnant Women ◽

Phosphatase Activity ◽

Alkaline Phosphatase Activity ◽

Lactate Production ◽

Glucose Consumption ◽

In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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