scholarly journals Nutrition and cellular immunity in hospital patients

1986 ◽  
Vol 55 (3) ◽  
pp. 515-527 ◽  
Author(s):  
Pauline S. Dowd ◽  
J. Kelleher ◽  
B. E. Walker ◽  
P. J. Guillou
Keyword(s):  
Vitamin C ◽  
Immune Function ◽  
Lymphocyte Proliferation ◽  
Nk Cell ◽  
Cell Activity ◽  
Autologous Serum ◽  
Pokeweed Mitogen ◽  
Neoplastic Disease ◽  
Nk Cell Activity ◽  
Con A

1. The interrelations between nutritional and ccllular immune function measurements were studied in seventy patients suffering from various degrees of malnutrition. They included patients with liver disease, inflammatory bowel disease, neoplastic disease, neurological patients, post-operative surgical patients and patients with respiratory problems.2. Nutritional measurements included: anthropometry, serum proteins, various vitamins and trace elements, and a prognostic nutritional index (PNI) was calculated.3. Immunological measurements included: (1) natural killer (NK) cell activity, (2) antibody-dependent cellular cytotoxicity (ADCC), (3) lymphocyte proliferation in response to the mitogens concanavalin A (Con A), phytohaemagglutinin (PHA) and pokeweed mitogen (PWM) in both AB and autologous serum.4. There was no association between anthropometric measurements and tests of immune function.5. The lymphocyte proliferation in response to mitogenic stimulation in the malnourished patients was depressed in autologous serum compared with the response of the same lymphocytes in pooled AB serum. The lymphocyte proliferation in response to Con A correlated with transferrin in autologous serum (r 0.46, n 49, P < 0.0 I ) and to a lesser extent in AB serum (r 0.33, n 51, P < 0.05). There was a difference in the Con A-stimulated tritiated-thymidine uptake between patients with low and normal serum zinc levels (P < 0.05) for cultures performed in autologous serum, but not AB serum.6. There was a significant correlation between NK cell activity and vitamin C (r 0.43, n 60, P < 0.01). There was no relation between nutritional measurements and ADCC or the lymphocyte response to stimulation with PHA or PWM.7. The results suggest that the severity of overall malnutrition does not influence several different aspects of the cellular immune response. However, the results do suggest that certain individual nutrients, particularly vitamin C and Zn, do influence the immunoreactivity of different lymphocyte subpopulations.

N-acetylcysteine does not affect the lymphocyte proliferation and natural killer cell activity responses to exercise

1998 ◽  
Vol 275 (4) ◽  
pp. R1227-R1231
Author(s):  
H. B. Nielsen ◽  
N. H. Secher ◽  
M. Kappel ◽  
B. K. Pedersen
Keyword(s):  
Natural Killer ◽  
Lymphocyte Proliferation ◽  
Nk Cell ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Nk Cell Activity ◽  
Double Blind ◽  
Significant Difference ◽  
Ergometer Rowing

This study evaluated whether N-acetylcysteine (NAC) attenuates the reduced lymphocyte proliferation and natural killer (NK) cell activity responses to exercise in humans. Fourteen oarsmen were double-blind randomized to either NAC (6 g daily for 3 days) or placebo groups. During 6-min “all-out” ergometer rowing, the concentration of lymphocytes in the peripheral blood increased, with no significant difference between NAC and placebo as reflected in lymphocyte subsets: CD4+, CD8+, CD16+, and CD19+ cells. The phytohemagglutinin-stimulated lymphocyte proliferation decreased from 9,112 ± 2,865 to 5,851 ± 1,588 cpm ( P < 0.05), but it was not affected by NAC. During exercise, the NK cell activity was elevated from 17 ± 3 to 38 ± 4% and it decreased to 7 ± 1% below the resting value 2 h into recovery. Yet, when evaluated as lytic units per CD16+ cell, the NK cell activity decreased during and after exercise without a significant effect of NAC. We conclude that NAC does not attenuate the reduction in lymphocyte proliferation and NK cell activity associated with intense exercise.

Clinical response augments NK cell activity independent of treatment modality: a randomized double-blind placebo controlled antidepressant trial

2004 ◽  
Vol 34 (3) ◽  
pp. 491-498 ◽  
Author(s):  
M. G. FRANK ◽  
S. E. HENDRICKS ◽  
W. J. BURKE ◽  
D. R. JOHNSON
Keyword(s):  
Immune Function ◽  
Clinical Response ◽  
Rating Scale ◽  
Cell Function ◽  
Nk Cell ◽  
Cell Activity ◽  
Therapeutic Modality ◽  
Nk Cell Activity ◽  
Double Blind ◽  
Double Blind Placebo

Background. Major depressive disorder (MDD) has been associated with alterations in immune function. Suppression of natural killer (NK) cell activity (NKCA) reliably characterizes immunological alterations observed in MDD. Antidepressant pharmacotherapy has been associated with modulation of NKCA. Previous investigations into antidepressant modulation of NKCA have not employed randomized double-blind placebo controlled designs. Thus, it is unknown whether treatment-associated changes in immune function are due to drug, placebo, or spontaneous remission effects. The present investigation examined the effect of antidepressant treatment on NKCA utilizing a randomized double-blind placebo controlled experimental design.Method. Patients (N=16) met DSM-IV criteria for MDD and were randomly assigned to drug (N=8; citalopram, 20 mg/day) or placebo (N=8) under double-blind conditions. Severity and pattern of depressive symptoms were assessed by the Hamilton Depression Rating Scale (HDRS). NK cell function was measured using a standard chromium-release assay and NK cell number assessed by flow cytometry. HDRS scores, NK cell function, and NK cell numbers were collected at 0, 1, 2 and 4 weeks of treatment.Results. Clinical response was associated with augmented NKCA independent of treatment condition. Failure to respond to treatment resulted in significantly reduced NKCA over treatment interval.Conclusions. The present results suggest that alterations in the depressive syndrome, regardless of therapeutic modality, may be sufficient to modulate NKCA during antidepressant trials and thus may significantly impact on co-morbid health outcomes in MDD.

NK Cell Activity and T-Lymphocyte Proliferation in Healthy Centenarians

1992 ◽  
Vol 663 (1 Aging and Cel) ◽  
pp. 505-507 ◽  
Author(s):  
P. SANSONI ◽  
V. BRIANTI ◽  
F. FAGNONI ◽  
G. SNELLI ◽  
A. MARCATO ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity ◽  
T Lymphocyte Proliferation

Effect of Acute Pain on Splenic NK Cell Activity, Lymphocyte Proliferation and Cytokine Production Activities

2007 ◽  
Vol 29 (3-4) ◽  
pp. 465-476 ◽  
Author(s):  
Ali Sharify ◽  
Mahmoud Mahmoudi ◽  
Maryam Hosseinali Izad ◽  
Mir-Jamal Hosseini ◽  
Mohammad Sharify
Keyword(s):  
Acute Pain ◽  
Lymphocyte Proliferation ◽  
Cytokine Production ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity

Effect of Jin Jiang Granules on Immune Function in Normal Mice

2013 ◽  
Vol 850-851 ◽  
pp. 1259-1262
Author(s):  
Ming San Miao ◽  
Rui Qi Li ◽  
Xue Xia Zhang
Keyword(s):  
Immune Function ◽  
Peripheral Blood ◽  
Blood Lymphocyte ◽  
Nk Cell ◽  
Early Stage ◽  
Cell Activity ◽  
Lymphocyte Transformation ◽  
Transformation Rate ◽  
Nk Cell Activity ◽  
Macrophage Phagocytosis

Objective: Explore the effect of functional food Jin Jiang granules on immune function in normal mice, to provide basis for Jin Jiang granules which are applied in early stage of cold. Methods: After normal mice were given to different doses of Jin Jiang granules, levels of peritoneal macrophage phagocytosis rate and indexes, thymus and spleen indexes, haemolysin, hemolytic plaque, peripheral blood lymphocyte transformation rate, NK cell activity were determined. Results: Compared with blank group, Jin Jiang granules can improve levels of peritoneal macrophage phagocytosis, organ indexes, haemolysin, hemolytic plaque, peripheral blood lymphocyte transformation rate, NK cell activity. Conclusion: Jin Jiang granules can improve the organisms immunity, relieve symptoms and promote recovery when applied in early stage of cold.

scholarly journals Immunomodulatory Polysaccharide fromChlorophytum borivilianumRoots

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Mayank Thakur ◽  
Paul Connellan ◽  
Myrna A. Deseo ◽  
Carol Morris ◽  
Vinod K. Dixit
Keyword(s):  
Immune Function ◽  
Mononuclear Cells ◽  
Nk Cell ◽  
Cell Activity ◽  
Water Soluble ◽  
Hot Water ◽  
Enzyme Linked Immunosorbent Assay ◽  
Albino Rats ◽  
Immunoglobulin Level ◽  
Nk Cell Activity

Chlorophytum borivilianumSantapau & Fernandes (Liliaceae) is an ayurvedicRasayanaherb with immunostimulating properties. The polysaccharide fraction (CBP) derived from hot water extraction ofC. borivilianum(CB), comprising of~31% inulin-type fructans and~25% acetylated mannans (of hot water-soluble extract), was evaluated for its effect on natural killer (NK) cell activity (in vitro). Human peripheral blood mononuclear cells (PBMCs), isolated from whole blood on a Ficoll-Hypaque density gradient, were tested in the presence or absence of varying concentrations of eachC. borivilianumfraction for modulation of NK cell cytotoxic activity toward K562 cells. Preliminary cytotoxicity evaluation against P388 cells was performed to establish non-cytotoxic concentrations of the different fractions.Testing showed the observed significant stimulation of NK cell activity to be due to the CBP ofC. borivilianum. Furthermore,in vivoevaluation carried out on Wistar strain albino rats for humoral response to sheep red blood cells (SRBCs) and immunoglobulin-level determination using enzyme-linked immunosorbent assay (ELISA), exhibited an effectiveness ofC. borivilianumaqueous extract in improving immune function. Present results provide useful information for understanding the role of CBP in modulating immune function.

Melatonin Fails to Modulate Immune Parameters Influenced by Calorie Restriction in Aging Fischer 344 Rats

2002 ◽  
Vol 227 (3) ◽  
pp. 201-207 ◽  
Author(s):  
Mohammad A. Pahlavani ◽  
Daniel A. Vargas ◽  
Ted R. Evans ◽  
Jian-hua Shu ◽  
James F. Nelson
Keyword(s):  
T Cell ◽  
Proliferative Response ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity ◽  
Con A ◽  
Fischer 344 ◽  
Immune Parameters ◽  
Old Rats ◽  
Ifn Γ

The aim of this study was to determine if long-term treatment with melatonin (MEL), a purported anti-aging agent, was as effective as calorie restriction (CR) in modulating immune parameters in aging Fischer 344 male rats. Splenic lymphocytes were isolated from 17-month-old rats that, beginning at 6 weeks of age, were treated with MEL (4 or 16 μg/ml in drinking water) and from 17-month-old rats fed ad libitum (AL) or rats fed a CR diet (55% of AL intake). The number of splenic T cell populations and T cell subsets was measured by flow cytometry, the proliferative response of splenocytes to Concanavalin A (Con A) and lipopolysaccharide (LPS) was measured by [3H]thymidine incorporation, and the induction of cytokine production (IL-2 and IFN-γ) was measured by ELISA assay. In addition, the level of the natural killer (NK) cell activity was assessed by fluorimetric assay. CR rats had a higher number of lymphocytes expressing the naïve T cell marker (CD3 OX22) than AL rats (P < 0.05). CR rats also showed greater induction of proliferative response, IL-2 and IFN-γ levels following Con A simulation, and NK cell activity than AL rats (P < 0.05). MEL-treated rats did not differ from AL rats in any of these parameters or in any other measurement. These results indicate that MEL treatment is unable to modulate immune function in a manner comparable with that of CR.

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