The effect of dietary bagasse on the activities of some key enzymes of carbohydrate and lipid metabolism in mouse liver
1. The effects of a 100 g/kg diet substitution of bagasse on the body-weight gain, food consumption and faecal dry weight of mice given a high-sucrose diet and on the activities of hepatic glucose-6-phosphate dehydrogenase (EC 1. 1. 1. 49), 6-phosphogluconate dehydrogenase (EC I. I. I. 44), malate dehydrogenase (oxaloacetatedecarboxylating) (NADP+) (EC I. I. I. 40), ATP-citrate (pro-3S) lyase (EC 4. 1. 3.8), 6-phosphofructokinase (EC 2. 7. 1. II), pyruvate kinase (EC 2.7. 1. 40) and fructose-1,6-bisphosphatase (EC 3. 1. 3. II) were studied.2. Bagasse had no effect on body-weight gain, food consumption or faecal dry weight.3. Bagasse decreased the activities of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and phosphofructokinase expressed on a wet weight basis and on a protein basis.4. Bagasse decreased the activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydro-genase expressed on a body-weight basis.5. These results suggest that bagasse decreases the flux through some pathways of hepatic lipogenesis when mice are given high-sucrose diets.