scholarly journals Effects of arginine and lysine addition to casein and soya-bean protein on serum lipids, apolipoproteins, insulin and glucagon in rats

1982 ◽  
Vol 48 (2) ◽  
pp. 211-221 ◽  
Author(s):  
M. Sugano ◽  
N. Ishiwaki ◽  
Y. Nagata ◽  
K. Imaizumi
Keyword(s):  
Serum Cholesterol ◽  
Serum Lipids ◽  
Serum Insulin ◽  
Serum Triglyceride ◽  
Protein Isolate ◽  
Soya Bean ◽  
Male Rats ◽  
Apo E ◽  
Arginine Supplementation ◽  
Bean Protein

1. The effect of variation in arginine: lysine on the relative cholesterolaemic effects of dietary soya-bean protein and casein was studied. Male rats received semi-purified diets containing soya-bean protein isolate or casein supplemented respectively with varingamounts of lysine or arginine for 40 d and blood samples were taken after a 5 h fast.2. Neither the addition of arginine to casein nor lysine to soya-bean protein modifiedthe intrinsic effect of these proteins on serum cholesterol.3. Serum triglyceride levels tended to rise with increasing amounts of lysine supplementation. The opposite trend was obtained with arginine supplementation.4. Casein caused an increase in the concentration of serum insulin, but not glucagon. The glucagon level was increased proportionately with increasing amounts of arginine, while the addition of lysine showed no effect. The effects of added amino acids on serum insulin were inconclusive.5. There was a parallel increase in serum apo E and glucagon in response to arginine supplementation, while lysine supplementation increased serum apo E.6. Thus, arginine:lysine was more effective in regulating serum triglyceride than serum cholesterol. Insulin was associated with different effects of these proteins on serum lipids.

scholarly journals Effects of soya-bean protein and casein on serum cholesterol levels in rats

1980 ◽  
Vol 44 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Y. Nagata ◽  
K. Imaizumi ◽  
M. Sugano
Keyword(s):  
Serum Cholesterol ◽  
Dietary Protein ◽  
Protein Isolate ◽  
Soya Bean ◽  
Male Rats ◽  
Cholesterol Levels ◽  
Definite Pattern ◽  
Neutral Sterols ◽  
The Difference ◽  
Bean Protein

1. The effect of the soya-bean protein isolate and casein, both given 200 g/kg diet for 3–4 weeks, on serum cholesterol was compared in male rats.2. Soya-bean protein exerted a hypocholesteraemic effect only in a cholesterol-free low-fat (10 g maize oil/kg) diet, when the lowering action appeared independent of the strain of the rat or the feeding pattern. The results obtained with diets containing cholesterol or higher levels of fats or both showed no definite pattern of response.3. Although the decrease in serum cholesterol appeared greater in α-lipoprotiens than in β-lipoproteins, the proportion of the former to total cholesterol remained almost unchanged. The concentration of serum apo A-I was significantly lower in rats given the vegetable protein.4. Rats given soya-bean protein excreted significantly more neutral sterols.5. The serum amino acid pattern did not reflect the difference in dietary protein. Addition of cholesterol to the diets modified the serum aminogarm, the decrease in threonine being most marked in both protein groups.6. This study shows that the hypocholesteraemic action of soya-bean protein is easily modified by the type of diet.

scholarly journals Digestibility of casein, formaldehyde-treated casein and soya-bean protein in relation to their effects on serum cholesterol in rabbits

1989 ◽  
Vol 62 (2) ◽  
pp. 331-342 ◽  
Author(s):  
Marja W. Kuyvenhoven ◽  
Wojciech F. Roszkowski ◽  
Clive E. West ◽  
Ron L. A. P. Hoogenboom ◽  
Ria M. E. Vos ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Serum Cholesterol ◽  
Important Determinant ◽  
Protein Digestibility ◽  
Protein Isolate ◽  
Soya Bean ◽  
Water Soluble ◽  
Casein Diet ◽  
Insoluble Protein ◽  
Bean Protein

Adult male rabbits were fed on semi-purified diets containing soya-bean protein isolate, casein or formaldehyde-treated casein as the protein source and 1 g cholesterol and 5 g of the non-absorbable marker chromic oxide/kg diet. The concentration of cholesterol in serum and in liver was increased on both the casein and formaldehyde-treated-casein diets. Excretion of bile acids and their concentration in faeces were lower in rabbits fed on casein or formaldehyde-treated casein when compared with rabbits fed on soya-bean protein. Apparent digestibility of nitrogen was lowest when formaldehyde-treated casein was fed, and highest on the casein diet. In rabbits fed on casein treated with formaldehyde, higher proportions of N were found in the water-soluble and trichloroacetic acid-insoluble protein fractions of the gastrointestinal tract contents compared with rabbits on the other two diets. Absorption of phosphate from the gastrointestinal tract was higher in rabbits fed on casein than in rabbits fed on soya-bean protein or formaldehyde-treated casein. The results indicate that, in rabbits, protein digestibility may not be an important determinant of serum cholesterol.

scholarly journals Dietary animal proteins and cholesterol metabolism in rabbits

1990 ◽  
Vol 64 (2) ◽  
pp. 473-485 ◽  
Author(s):  
Maria R. Lovati ◽  
Clive E. West ◽  
Cesare R. Sirtori ◽  
Anton C. Beynen
Keyword(s):  
Bile Acid ◽  
Whey Protein ◽  
Serum Cholesterol ◽  
Cholesterol Metabolism ◽  
Soya Bean ◽  
Liver Cholesterol ◽  
Fish Protein ◽  
High Background ◽  
Neutral Steroid ◽  
Bean Protein

The effect in rabbits of giving isonitrogenous purified diets containing casein, ovalbumin, fish protein, milk-whey protein and soya-bean protein were compared. The diets were balanced for cholesterol and for the amount and type of fat. When incorporated into low-cholesterol diets (0.8 g cholesterol/kg), casein, ovalbumin and soya-bean protein produced similar levels of serum cholesterol. With a high background of dietary cholesterol (1.5 g/kg), serum cholesterol concentrations increased with soya-bean protein, whey protein, casein and fish protein, in that order. Thus, the hypercholesterolaemic effect of casein in carefully balanced diets was only seen against a high-cholesterol background. The development of hypercholesterolaemia produced by giving fish protein was different from that produced by casein. First, less cholesterol accumulated in the very-low-density-lipoprotein fractions and more in the lipoproteins of higher density with fish protein than with casein. Second, fish protein, unlike casein, did not increase liver cholesterol. Third, transfer of rabbits from a diet containing soya-bean protein to one containing casein resulted in an immediate marked depression in neutral steroid and bile acid excretion in faeces. However, when rabbits were fed on the diet with fish protein after the diet with soya-bean protein, there was no significant depression in neutral steroid output and the depression in bile acid output was delayed. The present study suggests that different animal proteins cause hypercholesterolaemia by different mechanisms.

scholarly journals The effect of different feeds, including those containing soya-bean products, on the passage of digesta from the abomasum of the preruminant calf

1975 ◽  
Vol 33 (3) ◽  
pp. 329-349 ◽  
Author(s):  
R. H. Smith ◽  
J. W. Sissons
Keyword(s):  
Milk Protein ◽  
Protein Isolate ◽  
Soya Bean ◽  
Cow’S Milk ◽  
Serum Antibodies ◽  
Cow's Milk ◽  
Dietary Nitrogen ◽  
Bean Flour ◽  
Whole Milk ◽  
Bean Protein

1. The effects of various factors on rates of flow and composition of digesta leaving the abomasum of preruminant calves were studied. The possible relation of some of these effects to the development of serum antibodies to certain dietary constituents has also been examined. Two situations were distinguished: (a) unsensitized responses, shown by calves receiving milk protein or soya-bean products for the first one or two occasions; (b) sensitized responses, shown by calves receiving certain soya-bean products, after a number of these feeds had been given.2. For unsensitized calves, the rate of flow of total digesta from the abomasum was greater in the first few hours after a feed consisting of a mineral solution was given, than after cow's milk was given. This difference was apparently due to differences in the composition of digesta entering the duodenum. Total digesta flows after giving synthetic milk feeds, prepared from different protein sources, were similar to those after cow's milk was given.3. For sensitized calves, rates of flow of total digesta from the abomasum were greatly affected by the nature of the protein source used in the diet. Soya-bean flour (heated or unheated) generally caused inhibition of flow for some hours after feeding; a soya-bean protein isolate (isoelectric) had a similar but smaller effect, but a soya-bean concentrate (prepared by alcohol extraction of a soya-bean flour) and milk protein had little or no effect. The inhibition, believed to be a sign of more general disorders, appeared to be caused by a factor entering the duodenum which induced a change in the way in which the calf responded, probably as the result of a gastrointestinal allergy.4. Calves given soya-bean flour or a soya-bean protein isolate (isoelectric) in their diets for several weeks, showed respectively high and low titres of serum antibodies to an antigen prepared from soya-bean flour. Calves given alcohol-extracted soya-bean concentrate had no similar antibodies.5. In addition to variations in total digesta flow, dietary nitrogen compounds were held up in the abomasum to different extents after different feeds. After a whole-milk feed or a synthetic feed prepared from casein, a slow, steady release of N occurred over at least 9 h. N hold-up after giving soya-bean-containing feeds was slight for the soya-bean flour, but extremely marked for the soya-bean protein isolate (isoelectric). The latter hold-up was followed after several hours by a rapid outflow of N from the abomasum.

EFFECT OF PIPERAZINE OESTRONE SULPHATE ON SERUM LIPIDS AND LIPOPROTEINS IN MENOPAUSAL WOMEN

1977 ◽  
Vol 85 (1) ◽  
pp. 143-150 ◽  
Author(s):  
A. J. Isaacs ◽  
C. W. H. Havard
Keyword(s):  
Fatty Acid ◽  
Serum Cholesterol ◽  
Gonadal Dysgenesis ◽  
Serum Lipids ◽  
Total Phospholipid ◽  
Serum Triglyceride ◽  
Fasting Serum ◽  
Oestrone Sulphate ◽  
Menopausal Women ◽  
Lipids And Lipoproteins

ABSTRACT Twenty menopausal women and 2 women with gonadal dysgenesis were treated with piperazine oestrone sulphate 1.5–3 mg daily on a cyclical basis for a period of 6 months. Fasting serum lipids and lipoprotein esterified fatty acid indices (EFI) were estimated before starting treatment and after 3 and 6 months. There were small falls in serum cholesterol (significant at 3 months) and beta-lipoprotein EFI (significant at 6 months). Serum triglyceride and pre-beta-lipoprotein EFI rose significantly at both 3 and 6 months. Serum total phospholipid levels were reduced (significant at 6 months) with most marked changes in the sphingomyelin fraction. Other parameters were not significantly altered.

scholarly journals Digestibilities of casein and soya-bean protein in relation to their effects on serum cholesterol in rabbits

1985 ◽  
Vol 54 (2) ◽  
pp. 355-366 ◽  
Author(s):  
Christopher J. H. Woodward ◽  
Kenneth K. Carroll
Keyword(s):  
Heat Treatment ◽  
Serum Cholesterol ◽  
Soya Bean ◽  
Pancreatic Enzymes ◽  
Alkaline Ph ◽  
Acid Ph ◽  
Ph Dependent ◽  
Low Solubility ◽  
Bean Protein

1. Diets containing isolated soya-bean protein induce lower levels of serum cholesterol in animals than diets containing casein. Experiments were conducted to investigate whether differences in digestibility of the proteins might explain this effect.2. At pH 8 with pancreatic enzymes or intestinal peptidase, soya-bean protein was hydrolysed in vitro much less rapidly than casein. However, with pepsin (EC3. 4. 23. 1) at acid pH, soya-bean protein was hydrolysed more rapidly than casein.3. These differences in digestibility may be due to pH-dependent changes in solubility of the proteins. Casein and soya-bean protein were most soluble at alkaline and acid pH respectively.4. Heat treatment of the proteins resulted in lower solubilities and digestibilities. Sonication of soya-bean protein at pH 7.8 increased solubility but only slightly raised digestibility.5. When fed to rabbits, enzymically hydrolysed soya-bean protein induced a 2.3-fold higher concentration of serum cholesterol than did intact soya-bean protein. The hypocholesterolaemic effect of soya-bean protein may be partly attributable to its low solubility and digestibility at alkaline pH.

scholarly journals The effect of dietary protein source and saponins on serum lipids and the excretion of bile acids and neutral sterols in rabbits

1981 ◽  
Vol 46 (3) ◽  
pp. 421-430 ◽  
Author(s):  
Chitra Pathirana ◽  
M. J. Gibney ◽  
T. G. Taylor
Keyword(s):  
Dietary Protein ◽  
Serum Lipids ◽  
Milk Protein ◽  
Soya Bean ◽  
Protein Source ◽  
Plant Proteins ◽  
Lower Serum ◽  
Neutral Sterols ◽  
Dietary Protein Source ◽  
Bean Protein

1. Four groups of six rabbits were given purified diets in which the dietary variables were protein source (isolated soya-bean protein or cow's-milk protein) with or without saponin supplementation (10 g/kg).2. Rabbits given soya-based diets showed significantly lower serum concentrations of triglyceride and cholesterol (P < 0·01). The latter changes were confined to the cholesterol fractions of β-lipoproteins.3. These changes in serum cholesterol were associated with a significantly increased excretion of acid and neutral sterols in animals given soya-bean protein.4. There was no evidence to suggest that the effect of dietary protein source on serum lipids and the excretion of total and individual sterols was influenced by saponin supplementation.5. These results do not support the hypothesis that the hypocholesterolaemia associated with plant proteins is due to the presence of saponins.

scholarly journals The retention of 59Fe and 65Zn by preruminant lambs given milk-substitutes based on either casein or soya-bean-protein isolate

1985 ◽  
Vol 53 (3) ◽  
pp. 587-593
Author(s):  
R. Hill ◽  
D. M. Walker
Keyword(s):  
Zinc Chloride ◽  
Dietary Treatment ◽  
Protein Isolate ◽  
Soya Bean ◽  
Casein Diet ◽  
Milk Substitutes ◽  
Collection Period ◽  
The Difference ◽  
Protein Diets ◽  
Bean Protein

1. Eight newborn lambs were given milk-substitutes based on casein (n 4) or a soya-bean-protein isolate (n 4). On the morning before a 10 d collection period, the milk-substitutes were supplemented with 59Fe as ferric chloride and 5Zn as zinc chloride. Faeces and urine were collected daily and determinations of 59Fe and 65Zn were made on these separately. During a second 10 d period the dosing procedure and collection were repeated but the diet of each lamb was reversed.2. Mean retention of 59Fe in lambs given the casein diet was 0.50 (SE 0.12) and in those given the soya-bean-protein diet 0.06 (SE 0.05). There were large variations among animals but the treatment effect was significant at P < 0.05. Retention of 65Zn was significantly greater than that of 59Fe for all lambs but the effect of dietary treatment was similar to that for Fe. 65Zn retention values for casein and soya-bean-protein diets were 0.84 (E 0.06) and 0.52 (SE 0.03) respectively, and the difference was significant at P < 0.01.3. There were only very small amounts of 59Fe and 65Zn in urine, representing 0.002–0.003 of the dose.

scholarly journals Effect of Ashwagandharishta on the lipid profile of male and female rats

2021 ◽  
Vol 9 (1-2) ◽  
pp. 59-68
Author(s):  
Tasmina Rahman ◽  
Mohammad Salahuddin Bhuiya ◽  
Rakib Hasan ◽  
MSK Choudhuri
Keyword(s):  
Lipid Profile ◽  
Serum Cholesterol ◽  
Density Lipoprotein ◽  
Serum Triglyceride ◽  
Female Rats ◽  
Male Rats ◽  
Albino Rats ◽  
High Dose ◽  
Male And Female ◽  
Male And Female Rats

The effect of Ashwagandharishta on the lipid profile and chronic toxicity of both male and female Albino rats were observed for 51 days. Our results demonstrate that the treatment of the plant extract failed to exhibit any statistically significant change (increase/ decrease) in the serum cholesterol (Total), high density lipoprotein (HDL), low density lipoprotein (LDL) and triglyceride of the male rats. But the female rats showed highly (p<0.01) and very highly (p<0.001) significant decrease in the serum cholesterol (Total) levels of the medium and high dose treated groups respectively. Regarding HDL, ASG showed statistically significant decrease at low (p <0.05), medium (p<0.01) and high (p<0.001) dose treated female rats. The LDL level of the medium dose treated female rats were statistically significant (p <0.05). The serum Triglyceride decreased significantly at low (p<0.01) and high (p <0.05) dose treated female rats. Jahangirnagar University J. Biol. Sci. 9(1 & 2): 59-68, 2020 (June & December)

Food restriction as a modulator of age-related changes in serum lipids

1980 ◽  
Vol 238 (3) ◽  
pp. E253-E257 ◽  
Author(s):  
G. U. Liepa ◽  
E. J. Masoro ◽  
H. A. Bertrand ◽  
B. P. Yu
Keyword(s):  
Serum Cholesterol ◽  
Food Restriction ◽  
Serum Triglyceride ◽  
Serum Levels ◽  
Male Rats ◽  
Serum Free Fatty Acid ◽  
Young Rats ◽  
Age Related ◽  
Ad Libitum ◽  
Age Related Changes

Fischer 344 male rats were either fed ad libitum or 60% of the ad libitum intake. The restriction of food intake markedly increased the median length of life. Postabsorptive serum cholesterol and phospholipid concentrations increase in the ad libitum-fed rats with increasing age. Life-prolonging food restriction does not influence the serum levels of these lipids in young rats but delays the age-related increase in concentrations. Postabsorptive serum free fatty acid (FFA) concentrations decrease with advancing age in ad libitum-fed rats. Life-prolonging food restriction, while not affecting the serum FFA levels in young rats, delays and possibly partially prevents the age-related decrease in concentration. Food restriction lowers postabsorptive serum triglyceride levels at all ages studied. The data on serum cholesterol, phospholipids, and FFA provide further evidence that food restriction delays age-related changes in the physiological systems of rats. This delay of physiological decline may well retard the occurrence of age-related disease processes, thus prolonging life.

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